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Abstract:

Sensory analysis studies are critical in the development of quality enhanced crops, and may be an important component in the public acceptance of genetically modified foods. It has recently been established that odor preferences are shared between humans and mice, suggesting that odor exploration behavior in mice may be used to predict the effect of odors in humans. We have previously found that mice fed diets supplemented with engineered nonbrowning potatoes (-PPO) consumed more potato than mice fed diets supplemented with wild-type potatoes (WT). This prompted us to explore a possible role of potato odor in mice preference for nonbrowning potatoes. Taking advantage of two well established neuroscience paradigms, the ''open field test'' and the ''nose-poking preference test'', we performed experiments where mice exploration behavior was monitored in preference assays on the basis of olfaction alone. No obvious preference was observed towards -PPO or WT lines when fresh potato samples were tested. However, when oxidized samples were tested, mice consistently investigated -PPO potatoes more times and for longer periods than WT potatoes. Congruently, humans discriminated WT from -PPO samples with a considerably better performance when oxidized samples were tested than when fresh samples were tested in blind olfactory experiments. Notably, even though participants ranked all samples with an intermediate level of pleasantness, there was a general consensus that the -PPO samples had a more intense odor and also evoked the sense-impression of a familiar vegetable more often than the WT samples. Taken together, these findings suggest that our previous observations might be influenced, at least in part, by differential odors that are accentuated among the lines once oxidative deterioration takes place. Additionally, our results suggest that nonbrowning potatoes, in addition to their extended shelf life, maintain their odor quality for longer periods of time than WT potatoes. To our knowledge this is the first report on the use of an animal model applied to the sensory analysis of a transgenic crop. © 2010 Llorente et al.

Registro:

Documento: Artículo
Título:Improvement of aroma in transgenic potato as a consequence of impairing tuber browning
Autor:Llorente, B.; Rodríguez, V.; Alonso, G.D.; Torres, H.N.; Flawiá, M.M.; Bravo-Almonacid, F.F.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Buenos Aires, Argentina
Palabras clave:adult; animal experiment; aroma; article; controlled study; exploratory behavior; female; food preference; food quality; human; human experiment; mouse; nonhuman; normal human; olfactory discrimination; open field test; plant browning; potato; shelf life; smelling; transgenic crop; analysis of variance; animal; Bagg albino mouse; chemistry; color; food analysis; food preference; genetics; metabolism; odor; oxidation reduction reaction; physiology; plant tuber; potato; smelling; transgenic plant; Animalia; Mus; Solanum tuberosum; catechol oxidase; Analysis of Variance; Animals; Catechol Oxidase; Color; Exploratory Behavior; Female; Food Analysis; Food Preferences; Humans; Mice; Mice, Inbred BALB C; Odors; Olfactory Perception; Oxidation-Reduction; Plant Tubers; Plants, Genetically Modified; Smell; Solanum tuberosum
Año:2010
Volumen:5
Número:11
DOI: http://dx.doi.org/10.1371/journal.pone.0014030
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CAS:Catechol Oxidase, 1.10.3.1
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_19326203_v5_n11_p_Llorente.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v5_n11_p_Llorente

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Citas:

---------- APA ----------
Llorente, B., Rodríguez, V., Alonso, G.D., Torres, H.N., Flawiá, M.M. & Bravo-Almonacid, F.F. (2010) . Improvement of aroma in transgenic potato as a consequence of impairing tuber browning. PLoS ONE, 5(11).
http://dx.doi.org/10.1371/journal.pone.0014030
---------- CHICAGO ----------
Llorente, B., Rodríguez, V., Alonso, G.D., Torres, H.N., Flawiá, M.M., Bravo-Almonacid, F.F. "Improvement of aroma in transgenic potato as a consequence of impairing tuber browning" . PLoS ONE 5, no. 11 (2010).
http://dx.doi.org/10.1371/journal.pone.0014030
---------- MLA ----------
Llorente, B., Rodríguez, V., Alonso, G.D., Torres, H.N., Flawiá, M.M., Bravo-Almonacid, F.F. "Improvement of aroma in transgenic potato as a consequence of impairing tuber browning" . PLoS ONE, vol. 5, no. 11, 2010.
http://dx.doi.org/10.1371/journal.pone.0014030
---------- VANCOUVER ----------
Llorente, B., Rodríguez, V., Alonso, G.D., Torres, H.N., Flawiá, M.M., Bravo-Almonacid, F.F. Improvement of aroma in transgenic potato as a consequence of impairing tuber browning. PLoS ONE. 2010;5(11).
http://dx.doi.org/10.1371/journal.pone.0014030