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Abstract:

Naupactus cervinus (Boheman) (Curculionidae, Naupactini) is a parthenogenetic weevil native to the Paranaense Forest which displays high levels of genetic variation. Two divergent clades were identified, one ranging in forest areas (Forest clade), and the other in open vegetation areas (Grassland clade). Both of them have individuals with high levels of heterozygosity in ribosomal sequences. Investigation of intraindividual variation in ITS1 sequences through cloning and posterior sequencing suggested that mating between both groups most likely occurred in the Paranaense Forest after a secondary contact, which led to fixed heterozygotes as a consequence of parthenogenesis. Otherwise, sexual segregation would have disrupted multilocus genotypes. Only a small number of heterozygous genotypes of all the possible combinations are found in nature. We propose the occurrence of a hybrid zone in the Paranaense Forest. The fact that it is one of the most important biodiversity hotspots of the world, together with its key role for investigating evolutionary processes, makes it worthy of conservation. This is the first genetic evidence of bisexuality in N. cervinus. © 2018 Rodriguero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:A tale of swinger insects: Signatures of past sexuality between divergent lineages of a parthenogenetic weevil revealed by ribosomal intraindividual variation
Autor:Rodriguero, M.S.; Wirth, S.A.; Alberghina, J.S.; Lanteri, A.A.; Confalonieri, V.A.
Filiación:Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires—IEGEBA (CONICET-UBA), Buenos Aires, Argentina
Laboratorio de Agrobiotecnología, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires e, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA—CONICET/UBA), Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
División Entomología, Museo de La Plata, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata—CONICET, La Plata, Buenos Aires, Argentina
Palabras clave:adult; article; biodiversity; bisexuality; cladistics; controlled study; Curculionidae; forest; genetic association; genetic variation; genotype; grassland; heterozygote; human; hybrid zone; mating; nonhuman; parthenogenesis; vegetation; animal; genetic variation; genetics; molecular evolution; multigene family; phylogeny; physiology; reproduction; ribosome; sexual behavior; weevil; ribosomal spacer DNA; Animals; DNA, Ribosomal Spacer; Evolution, Molecular; Genetic Variation; Genotype; Heterozygote; Multigene Family; Phylogeny; Reproduction; Ribosomes; Sexual Behavior, Animal; Weevils
Año:2018
Volumen:13
Número:5
DOI: http://dx.doi.org/10.1371/journal.pone.0195551
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:DNA, Ribosomal Spacer
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v13_n5_p_Rodriguero

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Citas:

---------- APA ----------
Rodriguero, M.S., Wirth, S.A., Alberghina, J.S., Lanteri, A.A. & Confalonieri, V.A. (2018) . A tale of swinger insects: Signatures of past sexuality between divergent lineages of a parthenogenetic weevil revealed by ribosomal intraindividual variation. PLoS ONE, 13(5).
http://dx.doi.org/10.1371/journal.pone.0195551
---------- CHICAGO ----------
Rodriguero, M.S., Wirth, S.A., Alberghina, J.S., Lanteri, A.A., Confalonieri, V.A. "A tale of swinger insects: Signatures of past sexuality between divergent lineages of a parthenogenetic weevil revealed by ribosomal intraindividual variation" . PLoS ONE 13, no. 5 (2018).
http://dx.doi.org/10.1371/journal.pone.0195551
---------- MLA ----------
Rodriguero, M.S., Wirth, S.A., Alberghina, J.S., Lanteri, A.A., Confalonieri, V.A. "A tale of swinger insects: Signatures of past sexuality between divergent lineages of a parthenogenetic weevil revealed by ribosomal intraindividual variation" . PLoS ONE, vol. 13, no. 5, 2018.
http://dx.doi.org/10.1371/journal.pone.0195551
---------- VANCOUVER ----------
Rodriguero, M.S., Wirth, S.A., Alberghina, J.S., Lanteri, A.A., Confalonieri, V.A. A tale of swinger insects: Signatures of past sexuality between divergent lineages of a parthenogenetic weevil revealed by ribosomal intraindividual variation. PLoS ONE. 2018;13(5).
http://dx.doi.org/10.1371/journal.pone.0195551