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Abstract:

Psychrotroph microorganisms have developed cellular mechanisms to cope with cold stress. Cell envelopes are key components for bacterial survival. Outer membrane is a constituent of Gram negative bacterial envelopes, consisting of several components, such as lipopolysaccharides (LPS). In this work we investigated the relevance of envelope characteristics for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis by analyzing a mini Tn5 wapH mutant strain, encoding a core LPS glycosyltransferase. Our results showed that wapH strain is impaired to grow under low temperature but not for cold survival. The mutation in wapH, provoked a strong aggregative phenotype and modifications of envelope nanomechanical properties such as lower flexibility and higher turgor pressure, cell permeability and surface area to volume ratio (S/V). Changes in these characteristics were also observed in the wild type strain grown at different temperatures, showing higher cell flexibility but lower turgor pressure under cold conditions. Cold shock experiments indicated that an acclimation period in the wild type is necessary for cell flexibility and S/V ratio adjustments. Alteration in cell-cell interaction capabilities was observed in wapH strain. Mixed cells of wild type and wapH strains, as well as those of the wild type strain grown at different temperatures, showed a mosaic pattern of aggregation. These results indicate that wapH mutation provoked marked envelope alterations showing that LPS core conservation appears as a novel essential feature for active growth under cold conditions. © 2018 Benforte et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis
Autor:Benforte, F.C.; Colonnella, M.A.; Ricardi, M.M.; Solar Venero, E.C.; Lizarraga, L.; López, N.I.; Tribelli, P.M.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Centro de Investigaciones en Bionanociencias, CONICET, Buenos Aires, Argentina
Instituto de Fisiología, Biología Molecular y Neurociencias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
IQUIBICEN, CONICET, Buenos Aires, Argentina
Palabras clave:glycosyltransferase; lipopolysaccharide; glycosyltransferase; lipopolysaccharide; acclimatization; Article; bacterial growth; bacterial strain; cell flexibility; cell function; cell interaction; cell membrane permeability; cold acclimatization; cold shock response; controlled study; limit of quantitation; low temperature; nonhuman; phenotype; Pseudomonas; Pseudomonas extremaustralis; turgor pressure; adaptation; Antarctica; bacterial gene; cold; enzymology; genetics; metabolism; physiology; Pseudomonas; real time polymerase chain reaction; Adaptation, Physiological; Antarctic Regions; Cold Temperature; Genes, Bacterial; Glycosyltransferases; Lipopolysaccharides; Pseudomonas; Real-Time Polymerase Chain Reaction
Año:2018
Volumen:13
Número:2
DOI: http://dx.doi.org/10.1371/journal.pone.0192559
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:glycosyltransferase, 9033-07-2; Glycosyltransferases; Lipopolysaccharides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v13_n2_p_Benforte

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Citas:

---------- APA ----------
Benforte, F.C., Colonnella, M.A., Ricardi, M.M., Solar Venero, E.C., Lizarraga, L., López, N.I. & Tribelli, P.M. (2018) . Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis. PLoS ONE, 13(2).
http://dx.doi.org/10.1371/journal.pone.0192559
---------- CHICAGO ----------
Benforte, F.C., Colonnella, M.A., Ricardi, M.M., Solar Venero, E.C., Lizarraga, L., López, N.I., et al. "Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis" . PLoS ONE 13, no. 2 (2018).
http://dx.doi.org/10.1371/journal.pone.0192559
---------- MLA ----------
Benforte, F.C., Colonnella, M.A., Ricardi, M.M., Solar Venero, E.C., Lizarraga, L., López, N.I., et al. "Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis" . PLoS ONE, vol. 13, no. 2, 2018.
http://dx.doi.org/10.1371/journal.pone.0192559
---------- VANCOUVER ----------
Benforte, F.C., Colonnella, M.A., Ricardi, M.M., Solar Venero, E.C., Lizarraga, L., López, N.I., et al. Novel role of the LPS core glycosyltransferase WapH for cold adaptation in the Antarctic bacterium Pseudomonas extremaustralis. PLoS ONE. 2018;13(2).
http://dx.doi.org/10.1371/journal.pone.0192559