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Little is known about phylogeography of armadillo species native to southern South America. In this study we describe the phylogeography of the screaming hairy armadillo Chaetophractus vellerosus, discuss previous hypothesis about the origin of its disjunct distribution and propose an alternative one, based on novel information on genetic variability. Variation of partial sequences of mitochondrial DNA Control Region (CR) from 73 individuals from 23 localities were analyzed to carry out a phylogeographic analysis using neutrality tests, mismatch distribution, median-joining (MJ) network and paleontological records. We found 17 polymorphic sites resulting in 15 haplotypes. Two new geographic records that expand known distribution of the species are presented; one of them links the distributions of recently synonimized species C. nationi and C. vellerosus. Screaming hairy armadillo phylogeographic pattern can be addressed as category V of Avise: common widespread linages plus closely related lineages confined to one or a few nearby locales each. The older linages are distributed in the north-central area of the species distribution range in Argentina (i.e. ancestral area of distribution). C. vellerosus seems to be a low vagility species that expanded, and probably is expanding, its distribution range while presents signs of genetic structuring in central areas. To explain the disjunct distribution, a hypothesis of extinction of the species in intermediate areas due to quaternary climatic shift to more humid conditions was proposed. We offer an alternative explanation: long distance colonization, based on null genetic variability, paleontological record and evidence of alternance of cold/arid and temperate/humid climatic periods during the last million years in southern South America. © 2018 Poljak et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Documento: Artículo
Título:Phylogeography of screaming hairy armadillo Chaetophractus vellerosus: Successive disjunctions and extinctions due to cyclical climatic changes in southern South America
Autor:Poljak, S.; Ferreiro, A.M.; Chiappero, M.B.; Sánchez, J.; Gabrielli, M.; Lizarralde, M.S.
Filiación:Laboratorio de Ecología Molecular, Centro Austral de Investigaciones Científicas, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Ushuaia, Tierra del Fuego, Argentina
Instituto de Ciencias Polares Ambiente y Recursos Naturales, Universidad Nacional de Tierra del Fuego, Ushuaia, Tierra del Fuego, Argentina
Cátedra de Genética de Poblaciones y Evolución, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Diversidad y Ecología Animal, Córdoba, Argentina
Laboratorio de Ecología Molecular, Centro Regional de Estudios Genómicos, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina
Palabras clave:Argentina; armadillo; Article; Chaetophractus vellerosus; climate change; controlled study; gene structure; genetic variability; haplotype; nonhuman; phylogeography; species distribution; species extinction; animal; armadillo; climate; genetics; humidity; periodicity; phylogeography; South America; mitochondrial DNA; Animals; Armadillos; Climate; DNA, Mitochondrial; Extinction, Biological; Haplotypes; Humidity; Periodicity; Phylogeography; South America
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
CAS:DNA, Mitochondrial


  • Patterson, B., Pascual, R., The fossil mammal fauna of South America (1968) Q Rev Biol, 43 (4), pp. 409-451
  • Gibb, G.C., Condamine, F.L., Kuch, M., Enk, J., Moraes-Barros, N., Superina, M., Shotgun mitogenomics provides a reference phylogenetic framework and timescale for living xenarthrans (2015) Mol Biol Evol, 33 (3), pp. 621-642. ,, PMID: 26556496
  • Delsuc, F., Vizcaíno, S.F., Douzery, E.J., Influence of Tertiary paleoenvironmental changes on the diversification of South American mammals: A relaxed molecular clock study within xenarthrans (2004) BMC Evol Biol, 4 (1), p. 11
  • Delsuc, F., Superina, M., Tilak, M.-K., Douzery, E.J., Hassanin, A., Molecular phylogenetics unveils the ancient evolutionary origins of the enigmatic fairy armadillos (2012) Mol Phylogenet Evol, 62 (2), pp. 673-680. ,, PMID: 22122941
  • Poljak, S., Confalonieri, V., Fasanella, M., Gabrielli, M., Lizarralde, M.S., Phylogeography of the armadillo Chaetophractus villosus (Dasypodidae Xenarthra): Post-glacial range expansion from Pampas to Patagonia (Argentina) (2010) Mol Phyl Evol, 55 (1), pp. 38-46
  • Loughry, W., McDonough, C.M., (2013) The Nine-Banded Armadillo: A Natural History, , University of Oklahoma Press
  • Abba, A.M., Cassini, G.H., Valverde, G., Tilak, M.-K., Vizcaíno, S.F., Superina, M., Systematics of hairy armadillos and the taxonomic status of the Andean hairy armadillo (Chaetophractus nationi) (2015) J Mammal, , gyv082
  • Moraes-Barros, N., Arteaga, M.C., Genetic diversity in Xenarthra and its relevance to patterns of neotropical biodiversity (2015) J Mammal, 96 (4), pp. 690-702
  • Jr, G.D., Ecology of the little hairy armadillo Chaetophractus vellerosus (1985) The Evolution and Ecology of Armadillos, Sloths and Vermilinguas, pp. 397-405. , Montgomery GG, editor. Smithsonian Institution Press, Washington and London
  • Wetzel, R., Taxonomy and distribution of armadillos, Dasypodidae (1985) The Evolution and Ecology of Armadillos, Sloths, and Vermilinguas, pp. 23-46. , Montgomery GG, editor
  • Jr, G.D.H., Renal capabilities of an Argentine desert armadillo (1975) J Mammal, 56 (3), pp. 626-632
  • McNab, B.K., Energetics and the limits to a temperate distribution in armadillos (1980) J Mammal, 61 (4), pp. 606-627
  • Abba, A.M., Tognelli, M.F., Seitz, V.P., Bender, J.B., Vizcaíno, S.F., Distribution of extant xenarthrans (Mammalia: Xenarthra) in Argentina using species distribution models (2012) Mammalia, 76 (2), pp. 123-136
  • Carlini, A.A., Vizcaino, S.F., A new record of the armadillo Chaetophractus vellerosus (Gray, 1865)(Mam-malia, Dasypodidae) in the Buenos Aires Province of Argentine: Possible causes for the disjunct distribution (1987) Stud Neotrop Fauna E, 22 (1), pp. 53-56
  • Rabassa, J., Coronato, A.M., Salemme, M., Chronology of the Late Cenozoic Patagonian glaciations and their correlation with biostratigraphic units of the Pampean region (Argentina) (2005) J South Am Earth Sci, 20 (1), pp. 81-103
  • Isla, F.I., Holocene sea-level fluctuation in the southern hemisphere (1989) Quat Sci Rev, 8 (4), pp. 359-368
  • Marshall, L., Cifelli, R., Analysis of changing diversity patterns in Cenozoic land mammal age faunas, South America (1990) Palaeovertebrata, 19, pp. 169-210
  • Soibelzon, E., Carlini, A.A., Tonni, E.P., Soibelzon, L.H., Chaetophractus vellerosus (Mammalia: Dasypodidae) in the Ensenadan (Early-Middle Pleistocene) of the southeastern Pampean region (Argentina) (2006) Neues Jahrb Geol Paläontol
  • Cann, R.L., Stoneking, M., Wilson, A.C., Mitochondrial DNA and human evolution (1987) Nature, 325 (3)
  • Templeton, A.R., Routman, E., Phillips, C.A., Separating population structure from population history: A cladistic analysis of the geographical distribution of mitochondrial DNA haplotypes in the tiger salamander, Ambystoma tigrinum (1995) Genetics, 140 (2), pp. 767-782. , PMID: 7498753
  • Neigel, J.E., Avise, J.C., Application of a random walk model to geographic distributions of animal mitochondrial DNA variation (1993) Genetics, 135 (4), pp. 1209-1220. , PMID: 8307331
  • Neigel, J.E., Jr, B.R.M., Avise, J.C., Estimation of single generation migration distances from geographic variation in animal mitochondrial DNA (1991) Evolution, pp. 423-432. ,, PMID: 28567865
  • Nath, H., Griffiths, R., The coalescent in two colonies with symmetric migration (1993) J Math Biol, 31 (8), pp. 841-851. , PMID: 8263428
  • Slatkin, M., Inbreeding coefficients and coalescence times (1991) Genet Res, 58 (2), pp. 167-175
  • Slatkin, M., Isolation by distance in equilibrium and non-equilibrium populations (1993) Evolution, pp. 264-279. ,, PMID: 28568097
  • Avise, J.C., (2000) Phylogeography: The History and Duration of Species, , Harvard University Press, Cambridge
  • Frankham, R., Briscoe, D.A., Ballou, J.D., (2002) Introduction to Conservation Genetics, , Cambridge University Press
  • Huchon, D., Delsuc, F., Catzeflis, F.M., Douzery, E.J., Armadillos exhibit less genetic polymorphism in North America than in South America: Nuclear and mitochondrial data confirm a founder effect in Dasypus novemcinctus (Xenarthra) (1999) Mol Ecol, 8 (10), pp. 1743-1748. , PMID: 10583836
  • Glaz, D., Carlini, A., Estimación preliminar del home range y area máxima de Actividad en Chaetophractus vellerosus (Mammalia, Dasypodidae) (1999) XIV Jornadas Argentinas De Mastozoología Resúmenes, p. 18
  • Abba, A., Cassini, G., Cassini, M., Vizcaíno, S., Historia natural del piche llorón Chaetophractus vellerosus (Mammalia: Xenarthra: Dasypodidae) (2011) Rev Chil Hist Nat, 84 (1), pp. 51-64
  • Lyman, R.L., Paleozoology in the service of conservation biology (2006) Evol Anthrop, 15 (1), pp. 11-19
  • Shackleton, N., (1995) New Data on The Evolution of Pliocene Climatic Variability Paleoclimate and Evolution with Emphasis on Human Origins, pp. 242-248
  • Shackleton, N.J., Crowhurst, S., Hagelberg, T., Pisias, N.G., Schneider, D.A., A new late Neogene time scale: Application to Leg 138 sites (1995) Proc ODP, Sci Results
  • Soibelzon, E., Tonni, E.P., Early Pleistocene glaciations in Argentina (South America) and the response of the mammals: The case of the Pampean Region (2009) Curr Res Pleistocene, 26, pp. 175-177
  • Soibelzon, E., Using Paleoclimate and the Fossil Record to Explain Past and Present Distributions of Armadillos (Xenarthra, Dasypodidae) (2017) J Mammal Evol, pp. 1-10
  • Tonni, E., Mamíferos del Holoceno en la provincia de Buenos Aires (1990) Paula-Coutiana, 4, pp. 3-21
  • Tonni, E.P., Cione, A.L., Figini, A.B.J., Predominance of arid climates indicated by mammals in the pampas of Argentina during the Late Pleistocene and Holocene (1999) Palaeog Palaeocl Palaeoec, 147 (3), pp. 257-281
  • Soibelzon, E., Leon, D.C., Effects of climatic oscillations on the faunas. The Holocene Thermal Maximum and the displacement of armadillos in Argentina: Anatomical features and conservation (2017) J Archaeol Sci, 11, pp. 90-98
  • Deschamps, C.M., Late Cenozoic mammal bio-chronostratigraphy in southwestern Buenos Aires province, Argentina (2005) Ameghiniana, 42 (4), pp. 733-750
  • Tonni, E.P., Alberdi, M.T., Prado, J., Bargo, M.S., Cione, A.L., Changes of mammal assemblages in the pam-pean region (Argentina) and their relation with the Plio-Pleistocene boundary (1992) Palaeog Palaeocl Palaeoec, 95 (3-4), pp. 179-194
  • Vizcaíno, S.F., Bargo, M.S., Los armadillos (Mammalia, Dasypodidae) de La Toma (Partido de Coronel Príngles) y otros sitios arqueológicos de la provincia de Buenos Aires. Consideraciones paleoambien-tales (1993) Ameghiniana, 30 (4), pp. 435-443
  • Tonni, E.P., Carlini, A.A., Soibelzon, E., Soibelzon, L.H., Chaetophractus vellerosus (Mammalia: Dasypodidae) in the Ensenadan (Early-Middle Pleistocene) of the southeastern Pampean region (Argentina) (2006) Neues Jahrb Geol Paläontol, 12, pp. 734-748
  • Galíndez, E.J., Estecondo, S., Casanave, E.B., The spleen of a specially adapted mammal, the little hairy armadillo Chaetophractus vellerosus,(Xenarthra, Dasypodidae). A light and electron microscopic study (2006) Int J Morphol, 24 (3), pp. 339-348
  • Carlini, A.A., Krmpotic, C.M., Soibelzon, E., Scillato-Yané, G.J., Tolypeutes matacus, una primitiva especie viviente de reciente ingreso en latitudes medias (2003) Libro De Resúmenes. XVII Jornadas Argentinas De Mastozoología, p. 31. , La Rioja, Argentina: Sociedad Argentina para el Estudio de los Mamíferos (SAREM)
  • Soibelzon, E., Miño-Boilini, Á.R., Zurita, A.E., Krmpotic, C.M., Los Xenarthra (Mammalia) del Ensenadense (Pleistoceno inferior a medio) de la Región Pampeana (Argentina) (2010) Rev Mex Cienc Geol, 27 (3), pp. 449-469
  • Morrone, J.J., Biogeographic areas and transition zones of Latin America and the Caribbean islands based on panbiogeographic and cladistic analyses of the entomofauna (2006) Annu Rev Entomol, 51, pp. 467-494. ,, PMID: 16332220
  • Soibelzon, E., Medina, M., Abba, A.M., Late Holocene armadillos (Mammalia, Dasypodidae) of the Sierras of Córdoba, Argentina: Zooarchaeology, diagnostic characters and their paleozoological relevance (2013) Quaternary International, 299, pp. 72-79
  • Del Papa, L.M., De Santis, L., Togo, J., The Fossorial Faunal Record at the Beltrán Onofre Banegas-Lami Hernandez Archaeological Site (Santiago del Estero Province, Argentina): A Taphonomic Approach (2017) Zooarchaeology in The Neotropics: Environmental Diversity and Human-Animal Interactions, pp. 137-156. , M. M, editor. Springer
  • Del Papa, L.M., De Santis, L.J.M., Togo, J., Zooarqueología santiagueña. Despertando de la siesta (2011) Temas De Arqueología Estudios Tafonómicos Y Zooar-Queológicos (II), pp. 1-24. , Acosta A, Loponte D, Mucciolo L, editors. Buenos Aires: Asociación Amigos del Instituto Nacional de Antropología
  • Del Papa, L.M., De Santis, L.J.M., Togo, J., Zooarqueología del sitio Beltrán Cementerio, Santiago del Estero (2013) Cuadernos Del Instituto Nacional De Antropología Y Pensamiento Latinoamericano, 1 (1), pp. 168-180
  • Messineo, P.G., Scheifler, N.A., Investigaciones arqueológicas de cazadoresrecolectores en el sitio Laguna Cabeza de Buey 2.(centro de los pastizales pampeanos, Buenos Aires). Cincuenta años después de las Industrias Culturales definidas por Bórmida (2016) Intersecciones En Antropología, 17, pp. 213-226. , centro de los pastizales pampeanos, Buenos Aires
  • Sambrook, J., Fritsch, E., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , New York: Cold Spring Harbor Laboratory Press
  • Moraes-Barros, N., Morgante, J.S., A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections (2007) Genet Mol Biol, 30 (4), pp. 1181-1185
  • Vilà, C., Amorim, I.R., Leonard, J.A., Posada, D., Castroviejo, J., Petrucci-Fonseca, F., Mitochondrial DNA phylogeography and population history of the grey Wolf Canis lupus (1999) Mol Ecol, 8 (12), pp. 2089-2103. , PMID: 10632860
  • Hall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symposium Series, , editor London: Information Retrieval Ltd., c1979-c2000
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice (1994) Nucleic Acids Res, 22, pp. 4673-4680. , PMID: 7984417
  • Excoffier, L., Lischer, H.E., Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows (2010) Mol Ecol Res, 10 (3), pp. 564-567
  • Tajima, F., Measurement of DNA polymorphism (1993) Mechanisms of Molecular Evolution Introduction to Molecular Paleopopulation Biology, pp. 37-59. , Takahata N, Clark AG, editors. Japan Scientific Societies Press, Sinauer Associates, Tokyo/Sunderland, MA
  • Fu, Y.-X., Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection (1997) Genetics, 147 (2), pp. 915-925. , PMID: 9335623
  • Harpending, H., Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution (1994) Hum Biol, pp. 591-600. , PMID: 8088750
  • Bandelt, H.-J., Forster, P., Röhl, A., Median-joining networks for inferring intraspecific phylogenies (1999) Mol Biol Evol, 16 (1), pp. 37-48. , PMID: 10331250
  • Crandall, K.A., Templeton, A.R., Empirical tests of some predictions from coalescent theory with applications to intraspecific phylogeny reconstruction (1993) Genetics, 134 (3), pp. 959-969. , PMID: 8349118


---------- APA ----------
Poljak, S., Ferreiro, A.M., Chiappero, M.B., Sánchez, J., Gabrielli, M. & Lizarralde, M.S. (2018) . Phylogeography of screaming hairy armadillo Chaetophractus vellerosus: Successive disjunctions and extinctions due to cyclical climatic changes in southern South America. PLoS ONE, 13(1).
---------- CHICAGO ----------
Poljak, S., Ferreiro, A.M., Chiappero, M.B., Sánchez, J., Gabrielli, M., Lizarralde, M.S. "Phylogeography of screaming hairy armadillo Chaetophractus vellerosus: Successive disjunctions and extinctions due to cyclical climatic changes in southern South America" . PLoS ONE 13, no. 1 (2018).
---------- MLA ----------
Poljak, S., Ferreiro, A.M., Chiappero, M.B., Sánchez, J., Gabrielli, M., Lizarralde, M.S. "Phylogeography of screaming hairy armadillo Chaetophractus vellerosus: Successive disjunctions and extinctions due to cyclical climatic changes in southern South America" . PLoS ONE, vol. 13, no. 1, 2018.
---------- VANCOUVER ----------
Poljak, S., Ferreiro, A.M., Chiappero, M.B., Sánchez, J., Gabrielli, M., Lizarralde, M.S. Phylogeography of screaming hairy armadillo Chaetophractus vellerosus: Successive disjunctions and extinctions due to cyclical climatic changes in southern South America. PLoS ONE. 2018;13(1).