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Abstract:

Background Misiones Province in northeastern Argentina is considered to be a region with a high prevalence of HPV infection and a high mortality rate due to cervical cancer. The reasons for this epidemiological trend are not completely understood. To gain insight into this problem, we explored the relationship between mitochondrial DNA (mtDNA) ancestry, HPV infection, and development of cervical lesions/cancer in women from the city of Posadas in Misiones Province. Methods Two hundred and sixty-one women, including 92 cases of patients diagnosed with cervical lesions and 169 controls, were analyzed. mtDNA ancestry was assessed through HVS1 sequencing, while the detection and typing of HPV infection was conducted through nested multiplex PCR analysis. Multivariate logistic regression was conducted with the resulting data to estimate the odds ratios (ORs) adjusted by socio-demographic variables. Results The study participants showed 68.6% Amerindian, 26.1% European and 5.3% African mtDNA ancestry, respectively. Multiple regression analysis showed that women with African mtDNAs were three times more likely to develop a cervical lesion than those with Native American or European mtDNAs [OR of 3.8 (1.2–11.5) for ancestry and OR of 3.5 (1.0–12.0) for L haplogroups], although the associated p values were not significant when tested under more complex multivariate models. HPV infection and the development of cervical lesions/ cancer were significant for all tested models, with the highest OR values for HPV16 [OR of 24.2 (9.3–62.7)] and HPV-58 [OR of 19.0 (2.4–147.7)]. Conclusion HPV infection remains a central risk factor for cervical cancer in the Posadas population. The potential role of African mtDNA ancestry opens a new avenue for future medical association studies in multiethnic populations, and will require further confirmation in large-scale studies. © 2018 Badano et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Mitochondrial DNA ancestry, HPV infection and the risk of cervical cancer in a multiethnic population of northeastern Argentina
Autor:Badano, I.; Sanabria, D.J.; Totaro, M.E.; Rubinstein, S.; Gili, J.A.; Liotta, D.J.; Picconi, M.A.; Campos, R.H.; Schurr, T.G.
Filiación:Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Misiones, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Laboratory of Molecular Anthropology, Department of Anthropology, University of Pennsylvania, Philadelphia, United States
Laboratorio de Epidemiología Genética, Dirección de Investigación CEMIC-CONICET, Buenos Aires, Argentina
Servicio de Virus Oncogénicos, Departamento de Virología, INEI-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires, Argentina
Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:mitochondrial DNA; mitochondrial DNA; adult; African; aged; American Indian; ancestry group; Argentina; Article; cancer risk; controlled study; European; female; genetic risk; human; Human papillomavirus type 16; Human papillomavirus type 58; major clinical study; nonhuman; nucleotide sequence; papillomavirus infection; retrospective study; risk factor; uterine cervix cancer; very elderly; complication; ethnic group; ethnology; genetics; haplotype; multivariate analysis; papillomavirus infection; pathology; uterine cervix tumor; Adult; Argentina; DNA, Mitochondrial; Ethnic Groups; Female; Haplotypes; Humans; Multivariate Analysis; Papillomavirus Infections; Uterine Cervical Neoplasms
Año:2018
Volumen:13
Número:1
DOI: http://dx.doi.org/10.1371/journal.pone.0190966
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:DNA, Mitochondrial
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v13_n1_p_Badano

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Citas:

---------- APA ----------
Badano, I., Sanabria, D.J., Totaro, M.E., Rubinstein, S., Gili, J.A., Liotta, D.J., Picconi, M.A.,..., Schurr, T.G. (2018) . Mitochondrial DNA ancestry, HPV infection and the risk of cervical cancer in a multiethnic population of northeastern Argentina. PLoS ONE, 13(1).
http://dx.doi.org/10.1371/journal.pone.0190966
---------- CHICAGO ----------
Badano, I., Sanabria, D.J., Totaro, M.E., Rubinstein, S., Gili, J.A., Liotta, D.J., et al. "Mitochondrial DNA ancestry, HPV infection and the risk of cervical cancer in a multiethnic population of northeastern Argentina" . PLoS ONE 13, no. 1 (2018).
http://dx.doi.org/10.1371/journal.pone.0190966
---------- MLA ----------
Badano, I., Sanabria, D.J., Totaro, M.E., Rubinstein, S., Gili, J.A., Liotta, D.J., et al. "Mitochondrial DNA ancestry, HPV infection and the risk of cervical cancer in a multiethnic population of northeastern Argentina" . PLoS ONE, vol. 13, no. 1, 2018.
http://dx.doi.org/10.1371/journal.pone.0190966
---------- VANCOUVER ----------
Badano, I., Sanabria, D.J., Totaro, M.E., Rubinstein, S., Gili, J.A., Liotta, D.J., et al. Mitochondrial DNA ancestry, HPV infection and the risk of cervical cancer in a multiethnic population of northeastern Argentina. PLoS ONE. 2018;13(1).
http://dx.doi.org/10.1371/journal.pone.0190966