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Abstract:

In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural areas where soybean is the predominant crop. In such areas the pesticide Roundup Full II® (RU) is widely applied. The genotoxic effect of its active ingredient glyphosate (RU is 66.2% glyphosate) on the peripheral blood lymphocytes of C. villosus was tested over a range of concentrations (280, 420, 560, 1120 μmol/L). Culture medium without glyphosate served as negative control, while medium containing mitomycin C served as positive control. Genetic damage was characterized in terms of the percentage of cells with chromosome aberrations (CA), the mean number of sister chromatid exchanges (SCE) per cell, and the modification of cell proliferation kinetics via the calculation of the replication index. Significant increases (p < 0.0001) were seen in the CA frequency and the mean number of SCEs per cell compared to negative controls at all the RU concentrations tested. Chromatid breaks, the only form of CA observed, under the 560 μmol/L RU conditions and in presence of mitomycin C were four to five times more common than at lower concentrations, while no viable cells were seen in the 1120 μmol/L treatment. The mean number of SCEs per cell was significantly higher under the 280 μmol/L RU conditions than the 420 or 560 μmol/L RU conditions; cells cultivated in the presence of MMC also showed significantly more SCEs. All the RU concentrations tested (except in the 1120 μmol/L RU treatment [no viable cells]) induced a significant reduction in the replication index (p > 0.0001). The present results confirm the genotoxic effects of RU on C. villosus lymphocytes in vitro, strongly suggesting that exposure to RU could induce DNA damage in C. villosus wildlife. © 2017 Luaces et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies
Autor:Luaces, J.P.; Rossi, L.F.; Chirino, M.G.; Browne, M.; Merani, M.S.; Mudry, M.D.
Filiación:Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay, Ciudad Autónoma de Buenos Aires, Piso 10 Lab. 6, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz, Ciudad Autónoma de Buenos Aires, Argentina
Grupo de Citogenética de Insectos, Depto. Ecología, Genética y Evolución, FCENyN-UBA, Instituto de Ecología, Genética y Evolución de Buenos Aires. IEGEBA-CONICET, Ciudad Universitaria, Intendente Güiraldes, Ciudad Autónoma de Buenos Aires, Lab. 110. Piso 4 –Pabellón II, Argentina
Grupo de Investigación en Biología Evolutiva (GIBE), Depto. Ecología Genética y Evolución, FCEyN-UBA, Instituto de Ecología, Genética y Evolución de Buenos Aires. IEGEBA-CONICET, Ciudad Universitaria, Intendente Güiraldes, Ciudad Autónoma de Buenos Aires, Labs. 43–46. Piso 4—Pabellón II, Argentina
Palabras clave:glycine; glyphosate; pesticide; analogs and derivatives; animal; cell culture; chromosome breakage; DNA damage; DNA replication; drug effects; female; genetics; lymphocyte; male; sister chromatid exchange; Xenarthra; Animals; Cells, Cultured; Chromosome Breakage; DNA Damage; DNA Replication; Female; Glycine; Lymphocytes; Male; Pesticides; Sister Chromatid Exchange; Xenarthra
Año:2017
Volumen:12
Número:8
DOI: http://dx.doi.org/10.1371/journal.pone.0182911
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:glycine, 56-40-6, 6000-43-7, 6000-44-8; glyphosate, 1071-83-6; Glycine; glyphosate; Pesticides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v12_n8_p_Luaces

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Citas:

---------- APA ----------
Luaces, J.P., Rossi, L.F., Chirino, M.G., Browne, M., Merani, M.S. & Mudry, M.D. (2017) . Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies. PLoS ONE, 12(8).
http://dx.doi.org/10.1371/journal.pone.0182911
---------- CHICAGO ----------
Luaces, J.P., Rossi, L.F., Chirino, M.G., Browne, M., Merani, M.S., Mudry, M.D. "Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies" . PLoS ONE 12, no. 8 (2017).
http://dx.doi.org/10.1371/journal.pone.0182911
---------- MLA ----------
Luaces, J.P., Rossi, L.F., Chirino, M.G., Browne, M., Merani, M.S., Mudry, M.D. "Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies" . PLoS ONE, vol. 12, no. 8, 2017.
http://dx.doi.org/10.1371/journal.pone.0182911
---------- VANCOUVER ----------
Luaces, J.P., Rossi, L.F., Chirino, M.G., Browne, M., Merani, M.S., Mudry, M.D. Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies. PLoS ONE. 2017;12(8).
http://dx.doi.org/10.1371/journal.pone.0182911