Toscani, A.M.; Sampayo, R.G.; Barabas, F.M.; Fuentes, F.; Simian, M.; Leskow, F.C."Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models" (2017) PLoS ONE. 12(3)
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ERBB2 is a member of the ERBB family of tyrosine kinase receptors that plays a major role in breast cancer progression. Located at the plasma membrane, ERBB2 forms large clusters in spite of the presence of growth factors. Beta1 integrin, membrane receptor of extracellular matrix proteins, regulates adhesion, migration and invasiveness of breast cancer cells. Physical interaction between beta1 integrin and ERBB2 has been suggested although published data are contradictory. The aim of the present work was to study the interaction between ERBB2 and beta1 integrin in different scenarios of expression and activation. We determined that beta1 integrin and ERBB2 colocalization is dependent on the expression level of both receptors exclusively in adherent cells. In suspension cells, lack of focal adhesions leave integrins free to diffuse on the plasma membrane and interact with ERBB2 even at low expression levels of both receptors. In adherent cells, high expression of beta1 integrin leaves unbound receptors outside focal complexes that diffuse within the plasma membrane and interact with ERBB2 membrane domains. Superresolution imaging showed the existence of two distinct populations of ERBB2: a major population located in large clusters and a minor population outside these structures. Upon ERBB2 overexpression, receptors outside large clusters can freely diffuse at the membrane and interact with integrins. These results reveal how expression levels of beta1 integrin and ERBB2 determine their frequency of colocalization and show that extracellular matrix proteins shape membrane clusters distribution, regulating ERBB2 and beta1 integrin activity in breast cancer cells. © 2017 Toscani et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Documento: Artículo
Título:Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models
Autor:Toscani, A.M.; Sampayo, R.G.; Barabas, F.M.; Fuentes, F.; Simian, M.; Leskow, F.C.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina
CONICET, Universidad de Buenos Aires, Instituto de Química Biológica, Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
Universidad de Buenos Aires, Área Investigación, Instituto de Oncología Angel H. Roffo, Buenos Aires, Argentina
Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, Ciudad de Buenos Aires, C1425FQD, Argentina
Departamento de Ciencias Básicas, Universidad Nacional de Luján, Luján, Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de La Plata, La Plata, Buenos Aires, Argentina
Instituto de Nanosistemas and CEDSI, Universidad Nacional de San Martín, Campus Miguelete, San Martin, Buenos Aires, Argentina
Palabras clave:beta1 integrin; epidermal growth factor receptor 2; CD18 antigen; epidermal growth factor receptor 2; ERBB2 protein, human; Article; breast cancer cell line; cell adhesion; cell compartmentalization; cell membrane; confocal microscopy; enzyme localization; extracellular matrix; HeLa cell line; human; human cell; immunofluorescence; MCF-7 cell line; molecular imaging; protein analysis; protein expression; protein function; protein interaction; protein localization; protein structure; sequence analysis; SKBR3 cell line; T47D cell line; Western blotting; breast tumor; metabolism; pathology; tumor cell line; Breast Neoplasms; CD18 Antigens; Cell Line, Tumor; Humans; Receptor, ErbB-2
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
CAS:epidermal growth factor receptor 2, 137632-09-8; CD18 antigen, 172592-43-7; CD18 Antigens; ERBB2 protein, human; Receptor, ErbB-2


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---------- APA ----------
Toscani, A.M., Sampayo, R.G., Barabas, F.M., Fuentes, F., Simian, M. & Leskow, F.C. (2017) . Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models. PLoS ONE, 12(3).
---------- CHICAGO ----------
Toscani, A.M., Sampayo, R.G., Barabas, F.M., Fuentes, F., Simian, M., Leskow, F.C. "Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models" . PLoS ONE 12, no. 3 (2017).
---------- MLA ----------
Toscani, A.M., Sampayo, R.G., Barabas, F.M., Fuentes, F., Simian, M., Leskow, F.C. "Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models" . PLoS ONE, vol. 12, no. 3, 2017.
---------- VANCOUVER ----------
Toscani, A.M., Sampayo, R.G., Barabas, F.M., Fuentes, F., Simian, M., Leskow, F.C. Distinct ErbB2 receptor populations differentially interact with beta1 integrin in breast cancer cell models. PLoS ONE. 2017;12(3).