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Abstract:

Culex pipiens is the most cosmopolitanmosquito of the Pipiens Assemblage. By studying the nature of interactions between this species and microorganisms common to its breeding environment we can unravel important pitfalls encountered during development.We tested the survival rate of larval stages, pupae and adults of a Cx. pipiens colony exposed to a variety of microorganisms in laboratory conditions and assessed the transmission to offspring (F1) by those organisms that secured development up to adulthood. Three complementary experiments were designed to: 1) explore the nutritional value of yeasts and other microorganisms during Cx. pipiens development; 2) elucidate the transstadial transmission of yeast to the host offspring; and 3) to examine the relevance of all these microorganisms in female choice for oviposition-substratum. The yeast Saccharomyces cerevisiae proved to be the most nutritional diet, but despite showing the highest survival rates, vertical transmission to F1 was never confirmed. In addition, during the oviposition trials, none of the gravid females was attracted to the yeast substratum. Notably, the two native bacterial strains, Klebsiella sp. and Aeromonas sp., were the preferred oviposition media, the same two bacteria thatmanaged to feed neonates until molting into 2nd instar larvae. Our results not only suggest that Klebsiella sp. or Aeromonas sp. serve as attractants for oviposition habitat selection, but also nurture the most fragile instar, L1, to assure molting into a more resilient stage, L2, while yeast proves to be the most supportive diet for completing development. These experiments unearthed survival traits that might be considered in the future development of strategies of Cx. pipiens control. These studies can be extended to other members of the Pipiens Assemblage. © 2016 Díaz-Nieto et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Culex pipiens development is greatly influenced by native bacteria and exogenous yeast
Autor:Díaz-Nieto, L.M.; D'Alessio, C.; Perotti, M.A.; Berón, C.M.
Filiación:Mar del Plata- Argentina and Fundación para Investigaciones, Instituto de Investigaciones en Biodiversidad Y Biotecnología (INBIOTEC)-CONICET, Mar del Plata, Argentina
Argentina and Facultad de Ciencias Exactas Y Naturales, Fundación Instituto Leloir- Instituto de Investigaciones, UBA, Buenos Aires, Argentina
School of Biological Sciences, Ecology and Evolutionary Biology, University of Reading, Reading, United Kingdom
Palabras clave:adult; adulthood; Aeromonas; bacterial strain; breeding; case report; Culex pipiens; diet; egg laying; exposure; female; habitat selection; human; Klebsiella; larva; larval stage; mate choice; microorganism; molting; nutritional value; progeny; pupa; Saccharomyces cerevisiae; species; survival rate; vertical transmission; animal; bacterium; Culex; growth, development and aging; isolation and purification; male; microbiology; microflora; physiology; Animals; Bacteria; Culex; Female; Larva; Male; Microbiota; Molting; Oviposition; Saccharomyces cerevisiae
Año:2016
Volumen:11
Número:4
DOI: http://dx.doi.org/10.1371/journal.pone.0153133
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n4_p_DiazNieto

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Citas:

---------- APA ----------
Díaz-Nieto, L.M., D'Alessio, C., Perotti, M.A. & Berón, C.M. (2016) . Culex pipiens development is greatly influenced by native bacteria and exogenous yeast. PLoS ONE, 11(4).
http://dx.doi.org/10.1371/journal.pone.0153133
---------- CHICAGO ----------
Díaz-Nieto, L.M., D'Alessio, C., Perotti, M.A., Berón, C.M. "Culex pipiens development is greatly influenced by native bacteria and exogenous yeast" . PLoS ONE 11, no. 4 (2016).
http://dx.doi.org/10.1371/journal.pone.0153133
---------- MLA ----------
Díaz-Nieto, L.M., D'Alessio, C., Perotti, M.A., Berón, C.M. "Culex pipiens development is greatly influenced by native bacteria and exogenous yeast" . PLoS ONE, vol. 11, no. 4, 2016.
http://dx.doi.org/10.1371/journal.pone.0153133
---------- VANCOUVER ----------
Díaz-Nieto, L.M., D'Alessio, C., Perotti, M.A., Berón, C.M. Culex pipiens development is greatly influenced by native bacteria and exogenous yeast. PLoS ONE. 2016;11(4).
http://dx.doi.org/10.1371/journal.pone.0153133