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Abstract:

We investigated the effect of long-term starvation and posterior feeding on energetic reserves, oxidative stress, digestive enzymes, and histology of C. quadricarinatus midgut gland. The crayfish (6.27 g) were randomly assigned to one of three feeding protocols: continuous feeding throughout 80 day, continuous starvation until 80 day, and continuous starvation throughout 50 day and then feeding for the following 30 days. Juveniles from each protocol were weighed, and sacrificed at day 15, 30, 50 or 80. The lipids, glycogen, reduced glutathione (GSH), soluble protein, lipid peroxidation (TBARS), protein oxidation (PO), catalase (CAT), lipase and proteinase activities, and histology were measured on midgut gland. Starved crayfish had a lower hepatosomatic index, number ofmolts, specific growth rate, lipids, glycogen, and GSH levels than fed animals at all assay times. The starvation did not affect the soluble protein, TBARS, PO levels and CAT. In starved juveniles the lipase activity decreased as starvation time increased, whereas proteinase activity decreased only at day 80. The histological analysis of the starved animals showed several signs of structural alterations. After 30 days of feeding, the starved-feeding animals exhibited a striking recovery of hepatosomatic index, number of molts, lipids and glycogen, GSH, lipase activity and midgut gland structure. © 2016 Sacristán et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae)
Autor:Sacristán, H.J.; Ansaldo, M.; Franco-Tadic, L.M.; Gimenez, A.V.F.; Greco, L.S.L.
Filiación:Biology of Reproduction and Growth in Crustaceans, Department of Biodiversity and Experimental Biology, Buenos Aires University, Buenos Aires, Argentina
IBBEA, CONICET-UBA, Buenos Aires, Argentina
Ecophysiology and Ecotoxicology Laboratory, Argentinian Antarctic Institute, Buenos Aires, Argentina
Physiology of Aquatic Organisms, Institute of Marine and Coastal Research, CONICET, Mar del Plata University, Mar del Plata, Argentina
Palabras clave:catalase; glutathione; glycogen; lipid; protein; proteinase; thiobarbituric acid reactive substance; triacylglycerol lipase; catalase; glutathione; glycogen; lipid; peptide hydrolase; protein; triacylglycerol lipase; animal structures; Article; biochemistry; body weight; Cherax quadricarinatus; controlled study; crayfish; energy metabolism; enzyme activity; feeding; female; growth rate; hepatosomatic index; histology; juvenile animal; lipid peroxidation; metabolic parameters; midgut gland; molting; nonhuman; oxidation; oxidative stress; physiological process; starvation; animal; animal food; chemistry; enzymology; growth, development and aging; intestine mucosa; metabolism; pathology; physiology; Animal Feed; Animals; Astacoidea; Catalase; Glutathione; Glycogen; Intestinal Mucosa; Lipase; Lipid Peroxidation; Lipids; Oxidative Stress; Peptide Hydrolases; Proteins; Starvation
Año:2016
Volumen:11
Número:3
DOI: http://dx.doi.org/10.1371/journal.pone.0150854
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:catalase, 9001-05-2; glutathione, 70-18-8; glycogen, 9005-79-2; lipid, 66455-18-3; protein, 67254-75-5; proteinase, 9001-92-7; triacylglycerol lipase, 9001-62-1; peptide hydrolase; Catalase; Glutathione; Glycogen; Lipase; Lipids; Peptide Hydrolases; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Sacristan

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Citas:

---------- APA ----------
Sacristán, H.J., Ansaldo, M., Franco-Tadic, L.M., Gimenez, A.V.F. & Greco, L.S.L. (2016) . Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae). PLoS ONE, 11(3).
http://dx.doi.org/10.1371/journal.pone.0150854
---------- CHICAGO ----------
Sacristán, H.J., Ansaldo, M., Franco-Tadic, L.M., Gimenez, A.V.F., Greco, L.S.L. "Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae)" . PLoS ONE 11, no. 3 (2016).
http://dx.doi.org/10.1371/journal.pone.0150854
---------- MLA ----------
Sacristán, H.J., Ansaldo, M., Franco-Tadic, L.M., Gimenez, A.V.F., Greco, L.S.L. "Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae)" . PLoS ONE, vol. 11, no. 3, 2016.
http://dx.doi.org/10.1371/journal.pone.0150854
---------- VANCOUVER ----------
Sacristán, H.J., Ansaldo, M., Franco-Tadic, L.M., Gimenez, A.V.F., Greco, L.S.L. Long-term starvation and posterior feeding effects on biochemical and physiological responses of midgut gland of Cherax quadricarinatus juveniles (Parastacidae). PLoS ONE. 2016;11(3).
http://dx.doi.org/10.1371/journal.pone.0150854