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Abstract:

Information transmission in cells occurs quite accurately even when concentration changes are "read" by individual binding sites. In this paper we study ligand number and site occupancy fluctuations when ligands diffuse and react going beyond the analyses that focus on their asymptotic decay. In this way we show that, for immobile binding sites, fluctuations in the number of bound molecules decay on a relatively fast scale before the asymptotic behavior kicks in. This result can explain the observed co-existence of highly fluctuating instantaneous transcriptional activities with accumulated mRNA concentrations that have relatively small noise levels. We also show that the initial stages of the decay in the bound molecule number fluctuations have one or two characteristic timescales depending on the concentration of free molecules. This transition can explain the changes in enzyme activity observed at the single molecule level. © 2016 Pérez Ipiña, Ponce Dawson. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Fluctuations, correlations and the estimation of concentrations inside cells
Autor:Ipiña, E.P.; Dawson, S.P.
Filiación:Departamento de Física, FCEN-UBA, IFIBA, CONICET, Ciudad Universitaria, Pabellón I, Buenos Aires, 1428, Argentina
Palabras clave:Article; binding site; cell level; concentration (parameters); controlled study; enzyme activity; ligand binding; mathematical computing; molecular dynamics; process model; stochastic model; animal; biological model; biosynthesis; Drosophila melanogaster; genetic transcription; physiology; messenger RNA; Animals; Drosophila melanogaster; Models, Biological; RNA, Messenger; Transcription, Genetic
Año:2016
Volumen:11
Número:3
DOI: http://dx.doi.org/10.1371/journal.pone.0151132
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n3_p_Ipina

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Citas:

---------- APA ----------
Ipiña, E.P. & Dawson, S.P. (2016) . Fluctuations, correlations and the estimation of concentrations inside cells. PLoS ONE, 11(3).
http://dx.doi.org/10.1371/journal.pone.0151132
---------- CHICAGO ----------
Ipiña, E.P., Dawson, S.P. "Fluctuations, correlations and the estimation of concentrations inside cells" . PLoS ONE 11, no. 3 (2016).
http://dx.doi.org/10.1371/journal.pone.0151132
---------- MLA ----------
Ipiña, E.P., Dawson, S.P. "Fluctuations, correlations and the estimation of concentrations inside cells" . PLoS ONE, vol. 11, no. 3, 2016.
http://dx.doi.org/10.1371/journal.pone.0151132
---------- VANCOUVER ----------
Ipiña, E.P., Dawson, S.P. Fluctuations, correlations and the estimation of concentrations inside cells. PLoS ONE. 2016;11(3).
http://dx.doi.org/10.1371/journal.pone.0151132