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Abstract:

Scorpions of the genus Tityus show holokinetic chromosomes, achiasmatic male meiosis and an absence of heteromorphic sex chromosomes, like all Buthidae. In this work, we analysed the meiotic behaviour and chromosome rearrangements of a population of the scorpion Tityus confluens, characterising the cytotypes of males, females and embryos with different cytogenetic techniques. This revealed that all the females were structural homozygotes, while all the males were structural heterozygotes for different chromosome rearrangements. Four different cytotypes were described in males, which differed in chromosome number (2n = 5 and 2n = 6) and meiotic multivalent configurations (chains of four, five and six chromosomes). Based on a detailed mitotic and meiotic analysis, we propose a sequence of chromosome rearrangements that could give rise to each cytotype and in which fusions have played a major role. Based on the comparison of males, females and a brood of embryos, we also propose that the presence of multivalents in males and homologous pairs in females could be associated with the presence of cryptic sex chromosomes, with the male being the heterogametic sex. We propose that the ancestral karyotype of this species could have had homomorphic XY/XX (male/female) sex chromosomes and a fusion could have occurred between the Y chromosome and an autosome. © 2016 Adilardi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Registro:

Documento: Artículo
Título:Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions
Autor:Adilardi, R.S.; Ojanguren-Affilastro, A.A.; Mola, L.M.
Filiación:Laboratorio de Citogenética y Evolución, Departamento de Ecología, Genética y Evolución, IEGEBA (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
División de Aracnología, Museo Argentino de Ciencias Naturales Bernardino Rivadavia, CONICET, Ciudad Autónoma de Buenos Aires, Argentina
INBIOMED, CONICET-UBA, Facultad de Medicina, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:animal cell; animal tissue; Article; autosome; cell fusion; chromosome analysis; chromosome number; chromosome rearrangement; controlled study; cytotype; embryo; female; fluorescence in situ hybridization; heterozygosity; karyotyping; male; meiosis; mitosis; nonhuman; scorpion; sex chromosome; sex linkage; Tityus confluens; X chromosome; Y chromosome; animal; chromosome segregation; genetics; growth, development and aging; heterozygote; metabolism; nonmammalian embryo; scorpion; Animals; Chromosome Segregation; Embryo, Nonmammalian; Female; Heterozygote; In Situ Hybridization, Fluorescence; Karyotyping; Male; Meiosis; Mitosis; Scorpions; Sex Chromosomes
Año:2016
Volumen:11
Número:10
DOI: http://dx.doi.org/10.1371/journal.pone.0164427
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v11_n10_p_Adilardi

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Citas:

---------- APA ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A. & Mola, L.M. (2016) . Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions. PLoS ONE, 11(10).
http://dx.doi.org/10.1371/journal.pone.0164427
---------- CHICAGO ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A., Mola, L.M. "Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions" . PLoS ONE 11, no. 10 (2016).
http://dx.doi.org/10.1371/journal.pone.0164427
---------- MLA ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A., Mola, L.M. "Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions" . PLoS ONE, vol. 11, no. 10, 2016.
http://dx.doi.org/10.1371/journal.pone.0164427
---------- VANCOUVER ----------
Adilardi, R.S., Ojanguren-Affilastro, A.A., Mola, L.M. Sex-linked chromosome heterozygosity in males of Tityus confluens (Buthidae): A clue about the presence of sex chromosomes in scorpions. PLoS ONE. 2016;11(10).
http://dx.doi.org/10.1371/journal.pone.0164427