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Soares, A.P.G.; Guillin, E.A.; Borges, L.L.; Da Silva, A.C.T.; De Almeida, A.M.R.; Grijalba, P.E.; Gottlieb, A.M.; Bluhm, B.H.; De Oliveira, L.O. "More Cercospora species infect soybeans across the Americas than meets the eye" (2015) PLoS ONE. 10(8)
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Abstract:

Diseases of soybean caused by Cercospora spp. are endemic throughout the world's soybean production regions. Species diversity in the genus Cercospora has been underestimated due to overdependence on morphological characteristics, symptoms, and host associations. Currently, only two species (Cercospora kikuchii and C. sojina) are recognized to infect soybean; C. kikuchii causes Cercospora leaf blight (CLB) and purple seed stain (PSS), whereas C. sojina causes frogeye leaf spot. To assess cryptic speciation among pathogens causing CLB and PSS, phylogenetic and phylogeographic analyses were performed with isolates from the top three soybean producing countries (USA, Brazil, and Argentina; collectively accounting for ∼80% of global production). Eight nuclear genes and one mitochondrial gene were partially sequenced and analyzed. Additionally, amino acid substitutions conferring fungicide resistance were surveyed, and the production of cercosporin (a polyketide toxin produced bymany Cercospora spp.) was assessed. From these analyses, the longheld assumption of C. kikuchii as the single causal agent of CLB and PSS was rejected experimentally. Four cercosporin-producing lineages were uncovered with origins (about 1 Mya) predicted to predate agriculture. Some of the Cercospora spp. newly associated with CLB and PSS appear to represent undescribed species; others were not previously reported to infect soybeans. Lineage 1, which contained the ex-type strain of C. kikuchii, was monophyletic and occurred in Argentina and Brazil. In contrast, lineages 2 and 3 were polyphyletic and contained wide-host range species complexes. Lineage 4 was monophyletic, thrived in Argentina and the USA, and included the generalist Cercospora cf. flagellaris. Interlineage recombination was detected, along with a high frequency of mutations linked to fungicide resistance in lineages 2 and 3. These findings point to cryptic Cercospora species as underappreciated global considerations for soybean production and phytosanitary vigilance, and urge a reassessment of host-specificity as a diagnostic tool for Cercospora. Copyright: © 2015 Soares et al.

Registro:

Documento: Artículo
Título:More Cercospora species infect soybeans across the Americas than meets the eye
Autor:Soares, A.P.G.; Guillin, E.A.; Borges, L.L.; Da Silva, A.C.T.; De Almeida, A.M.R.; Grijalba, P.E.; Gottlieb, A.M.; Bluhm, B.H.; De Oliveira, L.O.
Filiación:Departamento de Bioquímica e Biologia Molecular, Universidade Federal de VIçosa, Viçosa (MG), Brazil
Instituto de Genética Ewald A. Favret, CNIA, INTA Castelar, Buenos Aires, Argentina
Empresa Brasileira de Pesquisa Agropecuária, Embrapa-Soja, Londrina (PR), Brazil
Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
University of Arkansas, Fayetteville, AK, United States
Palabras clave:amino acid substitution; antifungal resistance; Argentina; Article; Brazil; Cercospora; Cercospora kikuchii; Cercospora sojina; cfp gene; fungal gene; fungal plant disease; fungal strain; fungus culture; gene sequence; genetic analysis; genetic recombination; genetic variability; haplotype; mitochondrial gene; monophyly; nonhuman; phylogeny; phylogeography; soybean; tub gene; Ascomycetes; Bayes theorem; gene regulatory network; genetic variation; genetics; isolation and purification; Japan; microbiology; physiology; plant disease; plant leaf; plant seed; soybean; Western Hemisphere; mitochondrial DNA; Americas; Ascomycota; Bayes Theorem; DNA, Mitochondrial; Gene Regulatory Networks; Genes, Fungal; Genetic Variation; Haplotypes; Japan; Phylogeny; Plant Diseases; Plant Leaves; Recombination, Genetic; Seeds; Soybeans
Año:2015
Volumen:10
Número:8
DOI: http://dx.doi.org/10.1371/journal.pone.0133495
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:DNA, Mitochondrial
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n8_p_Soares

Referencias:

  • Pollack, F.G., An annotated compilation of Cercospora names (1987) Mycol Mem, 12, pp. 1-212
  • Crous, P.W., Braun, U., Mycosphaerella and its anamorphs. 1. Names published in Cercospora and Passalora (2003) CBS Biodiversity Series, 1, pp. 1-571
  • Fuckel, K.W.G.L., Fungi Rhenani exsiccati, Fasc. I-IV (1863) Hedwigia, 2, pp. 132-136
  • Goodwin, S.B., Dunkle, L.D., Zismann, V.L., Phylogenetic analysis of Cercospora and Mycosphaerella based on the internal transcribed spacer region of ribosomal DNA (2001) Phytopathology, 91, pp. 648-658. , 18942994
  • Chupp, C., (1954) A Monograph of the Fungus Genus Cercospora, , New York: The Ronald Press
  • Groenewald, J.Z., Nakashima, C., Nishikawa, J., Shin, H.D., Park, J.H., Jama, A.N., Species concepts in Cercospora: Spotting the weeds among the roses (2012) Stud Mycol., 75, pp. 115-170
  • Crous, P.W., Groenewald, J.Z., Groenewald, M., Caldwell, P., Braun, U., Harrington, T.C., Species of Cercospora associated with grey leaf spot of maize (2006) Stud Mycol., 55, pp. 189-197. , 18490979
  • Groenewald, M., Groenewald, J.Z., Crous, P.W., Distinct species exist within the Cercospora apii morphotype (2005) Phytopathology, 95, pp. 951-959. , 18944418
  • Hartman, G.L., Sinclair, J.B., Rupe, J.C., (1999) Compendium of Soybean Diseases, , Saint Paul: American Phytopathological Society APS Press
  • Ehrenshaft, M., Upchurch, R.G., Host protein (s) induces accumulation of the toxin cercosporin and mRNA in a phytopahtogenic strain of Cercospora kikuchii (1993) Physiol Mol Plant Pathol., 43, pp. 95-107
  • Matsumoto, T., Tomoyasu, R., Studies on the purple speck of soybean seed (1925) Ann Phytopathol Soc Jpn, 1, pp. 1-14
  • Walters, H.J., Soybean leaf blight caused by Cercospora kikuchii (1980) Plant Dis., 64, pp. 961-962
  • Daub, M.E., Ehrenshaft, M., The photoactivated Cercospora toxin cercosporin: Contributions to plant disease and fundamental biology (2000) Annu Rev Phytopathol., 38, pp. 461-490. , 11701851
  • Cai, G., Schneider, R.W., Vegetative compatibility groups in Cercospora kikuchii, the causal agent of Cercospora leaf blight and purple seed stain in soybean (2005) Phytopathology, 95, pp. 257-261. , 18943118
  • Cai, G., Schneider, R.W., Padgett, G.B., Assessment of lineages of Cercospora kikuchii in Louisiana for aggressiveness and screening soybean cultivars for resistance to Cercospora leaf blight (2009) Plant Dis., 93, pp. 868-874
  • Wrather, J.A., Anderson, T.R., Arsyad, D.M., Gai, J., Ploper, L.D., Porta-Puglia, A., Soybean disease loss estimates for the top 10 soybean producing countries in 1994 (1997) Plant Dis., 81, pp. 107-110
  • FAOSTAT, Statistics division (2014) Food and Agriculture Organization of the United Nations, , http://faostat3.fao.org/browse/Q/QC/E, Rome
  • Almeida, A.M.R., Piuga, F.F., Marin, S.R.R., Binneck, E., Sartori, F., Costamilan, L.M., Pathogenicity, molecular characterization, and cercosporin content of Brazilian isolates of Cercospora kikuchii (2005) Fitopatol Bras, 30, pp. 594-602
  • Lura, M.C., Latorre, M.G., Vaccari, R.M.C., Maumary, R., Soldano, A., Mattio, M., Genetic diversity of cercospora kikuchii isolates from soybean cultured in Argentina as revealed by molecular markers and cercosporin production (2011) Mycopathologia, 171, pp. 361-371. , 20835913
  • Imazaki, I., Homma, Y., Kato, M., Vallone, S., Yorinori, J.T., Henning, A.A., Genetic relationships between Cercospora kikuchii populations from South America and Japan (2006) Phytopathology, 96, pp. 1000-1008. , 18944056
  • Boyette, C.D., Walker, H.L., Production and storage of inoculum of Cercospora kikuchii for field studies (1985) Phytopathology, 75, pp. 183-185
  • Cai, G., Schneider, R.W., Population structure of Cercospora kikuchii, the causal agent of cercospora leaf blight and purple seed stain in soybean (2008) Phytopathology, 98, pp. 823-829. , 18943259
  • Dorrance, A.E., Draper, M.A., Hershman, D.E., (2007) Using Foliar Fungicides to Manage Soybean Rust, , United States: USDA
  • Ma, Z., Michailides, T.J., Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopahogentic fungi (2005) Crop Prot, 24, pp. 853-863
  • Davidson, R.M., Hanson, L.E., Franc, G.D., Panella, L., Analysis of β-tubulin Gene Fragments from Benzimidazolesensitive and.tolerant Cercospora beticola (2006) J Phytopathol., 154, pp. 321-328
  • Bolton, M.D., Rivera, V., Secor, G., Identification of the G143A mutation associated with QoI resistance in Cercospora beticola field isolates from Michigan, United States (2013) Pest Manag Sci., 69, pp. 35-39. , 22761173
  • Doyle, J.J., Doyle, J.L., Isolation of plant DNA from fresh tissue (1990) Focus, 12, pp. 13-15
  • Imazaki, I., Iizumi, H., Ishikawa, K., Sasahara, M., Yasuda, N., Koizumi, S., Effects of thiophanate-methyl and azoxystrobin on the composition of Cercospora kikuchii populations with thiophanate-methyl-resistant strains (2006) J Gen Plant Pathol., 72, pp. 292-300
  • Koressaar, T., Remm, M., Enhancements and modifications of primer design program primer3 (2007) Bioinformatics, 23, pp. 1289-1291. , 17379693
  • Imazaki, I., Ishikawa, K., Yasuda, N., Miyasaka, A., Kawasaki, S., Koizumi, S., Incidence of thiophanate-methyl resistance in Cercospora kikuchii within a single lineage based on amplified fragment length polymorphisms in Japan (2006) J Gen Plant Pathol., 72, pp. 77-84
  • Heath, L., Van Der Walt, E., Varsani, A., Martin, D.P., Recombination patterns in aphthoviruses mirror those found in other picornaviruses (2006) J Virol., 80, pp. 11827-11832. , 16971423
  • Nylander, J.A.A., MrModeltest v2[Software], , Program distributed by the author. Uppsala: Uppsala University, Evolutionary Biology Centre
  • Akaike, H., Information theory and an extension of maximum likelihood principle (1973) Second International Symposium on Information Theory, pp. 267-281. , Petrov BN, Csaki F, editors, Budapest: Akademiai Kiado
  • Ronquist, F., Huelsenbeck, J.P., MrBayes 3: Bayesian phylogenetic inference under mixed models (2003) Bioinformatics, 19, pp. 1572-1574. , 12912839
  • Drummond, A.J., Suchard, M.A., Xie, D., Rambaut, A., Bayesian phylogenetics with BEAUti and the BEAST 1.7 (2012) Mol Biol Evol., 29, pp. 1969-1973. , 22367748
  • Kasuga, T., White, T.J., Taylor, J.W., Estimation of nucleotide substitution rates in Eurotiomycete fungi (2002) Mol Biol Evol., 19, pp. 2318-2324. , 12446823
  • Bandelt, H.-J., Forster, P., Röhl, A., Median-joining networks for inferring intraspecific phylogenies (1999) Mol Biol Evol., 16, pp. 37-48. , 10331250
  • Librado, P., Rozas, J., DnaSP v5: A software for comprehensive analysis of DNA polymorphism data (2009) Bioinformatics, 25, pp. 1451-1452. , 19346325
  • Tajima, F., Statistical method for testing the neutral mutation hypothesis by DNA polymorphism (1989) Genetics, 123, pp. 585-595. , 2513255
  • Jenns, A.E., Daub, M.E., Upchurch, R.G., Regulation of cercosporin accumulation in culture by medium and temperature manipulation (1989) Phytopathology, 79, pp. 213-219
  • Avise, J.C., (2000) Phylogeography: The History and Formation of Species, , Cambridge, MA: Harvard University Press
  • Groenewald, M., Groenewald, J.Z., Harrington, T.C., Abeln, E.C.A., Crous, P.W., Mating type gene analysis in apparently asexual Cercospora species is suggestive of cryptic sex (2006) Fungal Genet Biol., 43, pp. 813-825. , 16839791
  • Kim, H., Newell, A.D., Cota-Sieckmeyer, R.G., Rupe, J.C., Fakhoury, A.M., Bluhm, B.H., Mating-type distribution and genetic diversity of Cercospora sojina populations on soybean from Arkansas: Evidence for potential sexual reproduction (2013) Phytopathology, 103, pp. 1045-1051. , 23721180
  • Roy, K.W., Abney, T.S., Purple seed stains of soybeans (1976) Phytopathology, 66, pp. 1045-1049
  • Jackson, E.W., Fenn, P., Chen, P., Inheritance of resistance to purple seed stain caused by Cercospora kikuchii in soybean PI 80837 (2006) Crop Sci., 46, pp. 1462-1466
  • Wilcox, J.R., Laviolette, F.A., Martin, R.J., Heritability of purple seed stain resistance in soybean (1975) Crop Sci., 15, pp. 525-526
  • Srisombun, S., Supapornhemin, P., (1993) Inheritance of Soybean Resistance to Purple Seed Stain, Soybean Genet Newsl, 20, pp. 92-93
  • Crous, P.W., Groenewald, J.Z., Hosts, species and genotypes: Opinions versus data (2005) Australas Plant Pathol., 34, pp. 463-470
  • Shivas, R.G., Cai, L., Cryptic fungal species unmasked (2012) Microbiology Australia, , http://journals.cambridgemedia.com.au/UserDir/CambridgeJournal/Articles/13shivas345.pdf, March 36-37
  • Quaedvlieg, W., Verkley, G.J.M., Shin, H.-D., Barreto, R.W., Alfenas, A.C., Swart, W.J., Sizing up Septoria (2013) Stud Mycol., 75, pp. 307-390. , 24014902
  • Rouxel, M., Mestre, P., Comont, G., Lehman, B.L., Schilder, A., Delmotte, F., Phylogenetic and experimental evidence for host-specialized cryptic species in a biotrophic oomycete (2013) New Phytol., 197, pp. 251-263. , 23153246
  • Stewart, J.E., Timmer, L.W., Lawrence, C.B., Pryor, B.M., Peever, T.L., Discord between morphological and phylogenetic species boundaries: Incomplete lineage sorting and recombination results in fuzzy species boundaries in an asexual fungal pathogen (2014) BMC Evol Biol., 14, p. 38. , 24593138

Citas:

---------- APA ----------
Soares, A.P.G., Guillin, E.A., Borges, L.L., Da Silva, A.C.T., De Almeida, A.M.R., Grijalba, P.E., Gottlieb, A.M.,..., De Oliveira, L.O. (2015) . More Cercospora species infect soybeans across the Americas than meets the eye. PLoS ONE, 10(8).
http://dx.doi.org/10.1371/journal.pone.0133495
---------- CHICAGO ----------
Soares, A.P.G., Guillin, E.A., Borges, L.L., Da Silva, A.C.T., De Almeida, A.M.R., Grijalba, P.E., et al. "More Cercospora species infect soybeans across the Americas than meets the eye" . PLoS ONE 10, no. 8 (2015).
http://dx.doi.org/10.1371/journal.pone.0133495
---------- MLA ----------
Soares, A.P.G., Guillin, E.A., Borges, L.L., Da Silva, A.C.T., De Almeida, A.M.R., Grijalba, P.E., et al. "More Cercospora species infect soybeans across the Americas than meets the eye" . PLoS ONE, vol. 10, no. 8, 2015.
http://dx.doi.org/10.1371/journal.pone.0133495
---------- VANCOUVER ----------
Soares, A.P.G., Guillin, E.A., Borges, L.L., Da Silva, A.C.T., De Almeida, A.M.R., Grijalba, P.E., et al. More Cercospora species infect soybeans across the Americas than meets the eye. PLoS ONE. 2015;10(8).
http://dx.doi.org/10.1371/journal.pone.0133495