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Abstract:

Solutions exchange systems are responsible for the timing of drug application on patch clamp experiments. There are two basic strategies for generating a solution exchange. When slow exchanges are bearable, it is easier to perform the exchange inside the tubing system upstream of the exit port. On the other hand, fast, reproducible, exchanges are usually performed downstream of the exit port. As both strategies are combinable, increasing the performance of upstream exchanges is desirable. We designed a simple method for manufacturing T-junctions (300 μm I.D.) and we measured the time profile of exchange of two saline solutions using a patch pipette with an open tip. Three factors were found to determine the timing of the solution switching: pressure, travelled distance and off-center distance. A linear relationship between the time delay and the travelled distance was found for each tested pressure, showing its dependence to the fluid velocity, which increased with pressure. The exchange time was found to increase quadratically with the delay, although a sizeable variability remains unexplained by this relationship. The delay and exchange times increased as the recording pipette moved away from the center of the stream. Those increases became dramatic as the pipette was moved close to the stream borders. Mass transport along the travelled distance between the slow fluid at the border and the fast fluid at the center seems to contribute to the time course of the solution exchange. This effect would be present in all tubing based devices. Present results might be of fundamental importance for the adequate design of serial compound exchangers which would be instrumental in the discovery of drugs that modulate the action of the physiological agonists of ion channels with the purpose of fine tuning their physiology. Copyright: © 2015 Auzmendi et al.

Registro:

Documento: Artículo
Título:Dynamics of T-junction solution switching aimed at patch clamp experiments
Autor:Auzmendi, J.A.; Smoler, M.; Moffatt, L.
Filiación:Instituto de Química Física de Los Materiales, Medio Ambiente y Energía, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Canales Iónicos, Cátedra de Biofísica, Facultad de Odontología, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:sodium chloride; solution and solubility; Article; controlled study; equipment design; experimental study; fluid transport; laboratory device; mathematical model; molecular dynamics; molecular interaction; patch clamp technique; pipette; T-junction solution exhanger; tube; devices; pressure; procedures; solution and solubility; time factor; Patch-Clamp Techniques; Pressure; Solutions; Time Factors
Año:2015
Volumen:10
Número:7
DOI: http://dx.doi.org/10.1371/journal.pone.0133187
Título revista:PLoS ONE
Título revista abreviado:PLoS ONE
ISSN:19326203
CODEN:POLNC
CAS:sodium chloride, 7647-14-5; Solutions
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19326203_v10_n7_p_Auzmendi

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Citas:

---------- APA ----------
Auzmendi, J.A., Smoler, M. & Moffatt, L. (2015) . Dynamics of T-junction solution switching aimed at patch clamp experiments. PLoS ONE, 10(7).
http://dx.doi.org/10.1371/journal.pone.0133187
---------- CHICAGO ----------
Auzmendi, J.A., Smoler, M., Moffatt, L. "Dynamics of T-junction solution switching aimed at patch clamp experiments" . PLoS ONE 10, no. 7 (2015).
http://dx.doi.org/10.1371/journal.pone.0133187
---------- MLA ----------
Auzmendi, J.A., Smoler, M., Moffatt, L. "Dynamics of T-junction solution switching aimed at patch clamp experiments" . PLoS ONE, vol. 10, no. 7, 2015.
http://dx.doi.org/10.1371/journal.pone.0133187
---------- VANCOUVER ----------
Auzmendi, J.A., Smoler, M., Moffatt, L. Dynamics of T-junction solution switching aimed at patch clamp experiments. PLoS ONE. 2015;10(7).
http://dx.doi.org/10.1371/journal.pone.0133187