Artículo

Regueira, E.; Sassone, A.G.; Scaia, M.F.; Volonteri, M.C.; Ceballos, N.R. "Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum" (2013) Journal of Experimental Zoology Part A: Ecological Genetics and Physiology. 319(1):39-52
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Abstract:

Several studies indicate that wild free-living vertebrates seasonally regulate plasma glucocorticoids. However, not only glucocorticoids but also the amount of receptors is important in determining biological responses. In this context, seasonal regulation of glucocorticoid receptor (GR) is crucial to modulate the response to glucocorticoids. Rhinella arenarum is an anuran exhibiting seasonal variations in plasma glucocorticoids and also in the number of binding sites (Bmax) of the testicular cytosolic GR. In this work, we evaluated if the annual pattern of GR protein in the testis varies seasonally and, by an in vitro approach, the role of glucocorticoids, androgens, and melatonin in the regulation of the GR Bmax and protein level. For this purpose, testes were treated with two physiological concentrations of melatonin (40 and 200pg/ml), with or without luzindole (melatonin-receptor antagonist); with testosterone, cyanoketone (inhibitor of steroidogenesis) or casodex (androgen-receptor antagonist); or with dexamethasone or RU486 (GR antagonist). After treatments, Bmax and protein level were determined by the binding of [3H]dexamethasone and Western blot, respectively. Results showed that GR protein decreases in the winter. The in vitro treatment with melatonin produced a biphasic effect on the Bmax with the lowest concentration decreasing this parameter by a receptor-mediated mechanism. However, melatonin had no effect on the GR protein level. Conversely, a high concentration of dexamethasone up-regulated the GR protein and androgens neither changed the Bmax nor the protein level. These findings suggest that seasonal changes in plasma melatonin and glucocorticoids modulate the effect of glucocorticoids in the testis of R. arenarum. © 2012 Wiley Periodicals, Inc.

Registro:

Documento: Artículo
Título:Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum
Autor:Regueira, E.; Sassone, A.G.; Scaia, M.F.; Volonteri, M.C.; Ceballos, N.R.
Filiación:Laboratorio de Endocrinologia Comparada, Departamento de Biodiversidad y Biologia Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Palabras clave:anilide; bicalutamide; cyanoketone; dexamethasone; glucocorticoid; glucocorticoid receptor; luzindole; melatonin; mifepristone; nitrile; testosterone; toluenesulfonic acid derivative; tryptamine derivative; animal; animal disease; article; binding site; blood; Bufo marinus; drug effect; gene expression regulation; genetics; in vitro study; kinetics; male; metabolism; randomization; season; testis; Western blotting; Anilides; Animals; Binding Sites; Blotting, Western; Bufo marinus; Cyanoketone; Dexamethasone; Gene Expression Regulation; Glucocorticoids; Kinetics; Male; Melatonin; Mifepristone; Nitriles; Random Allocation; Receptors, Glucocorticoid; Seasons; Testis; Testosterone; Tosyl Compounds; Tryptamines; Anura; Vertebrata
Año:2013
Volumen:319
Número:1
Página de inicio:39
Página de fin:52
DOI: http://dx.doi.org/10.1002/jez.1772
Título revista:Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
Título revista abreviado:J. Exp. Zool. Part A Ecol. Genet. Physiol.
ISSN:19325223
CAS:bicalutamide, 90357-06-5; cyanoketone, 4248-66-2; dexamethasone, 50-02-2; luzindole, 117946-91-5; melatonin, 73-31-4; mifepristone, 84371-65-3; testosterone, 58-22-0; tryptamine derivative, 1019-45-0; Anilides; Cyanoketone, 4248-66-2; Dexamethasone, 50-02-2; Glucocorticoids; Melatonin, 73-31-4; Mifepristone, 84371-65-3; Nitriles; Receptors, Glucocorticoid; Testosterone, 58-22-0; Tosyl Compounds; Tryptamines; bicalutamide, 90357-06-5; luzindole, 117946-91-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19325223_v319_n1_p39_Regueira

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Citas:

---------- APA ----------
Regueira, E., Sassone, A.G., Scaia, M.F., Volonteri, M.C. & Ceballos, N.R. (2013) . Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum. Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 319(1), 39-52.
http://dx.doi.org/10.1002/jez.1772
---------- CHICAGO ----------
Regueira, E., Sassone, A.G., Scaia, M.F., Volonteri, M.C., Ceballos, N.R. "Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum" . Journal of Experimental Zoology Part A: Ecological Genetics and Physiology 319, no. 1 (2013) : 39-52.
http://dx.doi.org/10.1002/jez.1772
---------- MLA ----------
Regueira, E., Sassone, A.G., Scaia, M.F., Volonteri, M.C., Ceballos, N.R. "Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum" . Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, vol. 319, no. 1, 2013, pp. 39-52.
http://dx.doi.org/10.1002/jez.1772
---------- VANCOUVER ----------
Regueira, E., Sassone, A.G., Scaia, M.F., Volonteri, M.C., Ceballos, N.R. Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum. J. Exp. Zool. Part A Ecol. Genet. Physiol. 2013;319(1):39-52.
http://dx.doi.org/10.1002/jez.1772