Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Drought greatly affects the growth and development of plants. This stressful condition can trigger an increase in reactive oxygen species (ROS) production that, in turn, can induce cellular, anatomical, and morphological changes that improve drought tolerance. A strain of arbuscular mycorrhizal fungi (AMF) is considered efficient when it colonizes roots quickly and extensively, absorbs and transfers nutrients to the plant host, promotes soil aggregation, and protects the host against disease. We evaluated the effect of inoculation of two strains of the AMF Rhizophagus intraradices (N.C. Schenck & G.S. Smith) C. Walker & A. Schüßler (GA5 and GC2) on pomegranate plants (Punica granatum L.) under two irrigation conditions. The response to oxidative stress depended on many factors, including the organism tissue and the degree of stress. Our study showed that, in most cases, mycorrhizal plants increased antioxidant defenses, such as the ROS-scavenging enzymes superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) in shoots under both irrigation levels, whereas the response for roots was ambiguous. AMF inoculation maintained the levels of malondialdehyde (MDA), probably by rapidly increasing antioxidant defenses and preventing lipid damage. We show that early AMF inoculation (particularly with the GC2 strain) in pomegranate propagation protects plants against abiotic stress.

Registro:

Documento: Artículo
Título:Arbuscular mycorrhizal fungi alleviate oxidative stress in pomegranate plants growing under different irrigation conditions
Autor:Bompadre, M.J.; Silvani, V.A.; Bidondo, L.F.; Ríos de Molina, M.D.C.; Colombo, R.P.; Pardo, A.G.; Godeas, A.M.
Filiación:Departamento de Biodiversidad y Biología Experimental, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, 4to piso, Pabellón 2, C1428EGA, Buenos Aires, Argentina
Departamento de Química Biológica IQUIBICEN, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, 4to piso, Pabellón 2,, C1428EGA, Buenos Aires, Argentina
Laboratorio de Micología Molecular, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD Bernal, Buenos Aires, Argentina
Palabras clave:Arbuscular mycorrhizal fungi; Ascorbate peroxidase; Catalase; Glutathione reductase; Pomegranate; Superoxide dismutase; antioxidant; arbuscular mycorrhiza; drought stress; environmental stress; enzyme activity; fruit; fungus; growth rate; irrigation; oxygen; phytochemistry; root colonization; soil aggregate; tick; woody plant
Año:2014
Volumen:92
Número:3
Página de inicio:187
Página de fin:193
DOI: http://dx.doi.org/10.1139/cjb-2013-0169
Título revista:Botany
Título revista abreviado:Bot.
ISSN:19162804
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_19162804_v92_n3_p187_Bompadre

Referencias:

  • Aebi, H., Catalase in vitro (1984) Methods Enzymol, 105, pp. 121-126. , doi:10.1016/S0076-6879(84)05016-3. PMID:6727660
  • Alguacil, M.M., Hernández, J.A., Caravaca, F., Portillo, B., Roldán, A., Antioxidant enzyme activities in shoots from three mycorrhizal shrub species afforested in a degraded semi-arid soil (2003) Physiol. Plant, 118, pp. 562-570. , doi:10.1034/j.1399-3054.2003.00149.x
  • Beyer Jr., W.F., Fridovich, I., Assaying for superoxide dismutase activity: Some large consequences ofminor changes in conditions (1987) Anal. Biochem, 161, pp. 559-566. , doi:10.1016/0003-2697(87)90489-1. PMID:3034103
  • Bradford, M.M., A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding (1976) Anal. Biochem, 72, pp. 248-254. , doi:10.1016/0003-2697(76)90527-3. PMID: 942051
  • Bressano, M., Curetti, M., Giachero, L., Vargas Gil, S., Cabello, M., March, G., Ducasse, D.A., Luna, C.M., Mycorrhizal fungi symbiosis as a strategy against oxidative stress in soybean plants (2010) Plant Physiol, 167, pp. 1622-1626. , doi:10.1016/j.jplph.2010.06.024. PMID:20801548
  • Carlberg, I., Mannervik, B., Glutathione reductase (1985) Methods Enzymol, 113, pp. 484-490. , doi:10.1016/S0076-6879(85)13062-4. PMID:3003504
  • Causin, H.F., Roberts, I.N., Criado, M.V., Gallego, S.M., Pena, L.B., Ríos, M.D.C., Barneix, A.J., Changes in hydrogen peroxide homeostasis and cyto-kinin levels contribute to the regulation of shade-induced senescence in wheat leaves (2009) J. Plant Sci, 177, pp. 698-704. , doi:10.1016/j.plantsci.2009.08.014
  • Chopade, S.O., Gorantiwar, S.D., Pampattiwar, P.S., Supe, V.S., Response of pomegranate to drip, bubbler and surface irrigation methods (2001) Advances In Horticulture and Forestry. Sci. Pub. India Jodhpur, 8, pp. 53-59
  • Clewer, A.G., Scarisbrick, D.H., Factorial experiments (2001) Practical Statistics and Experimental Design For Plant and Crop Science, pp. 159-181. , Edited by John Wiley and Sons Ltd. The Atrium, Southern Gate, Chicheste, West Sussex, England
  • Davies Jr., F.T., Olade-Portugal, V., Aguilera-Gomez, L., Alvarado, M.J., Ferrera-Cerrato, R.C., Boutton, T.W., Alleviationof drought stress of Chile ancho pepper (Capsicum annuum L. cv. San Luis) with arbuscular mycorrhiza indigenous to Mexico (2002) Sci. Hortic, 92, pp. 347-359. , doi:10.1016/ S0304-4238(01)00293-X
  • Dumas-Gaudot, E., Gollote, A., Cordier, C., Gianinazzi, S., Gianinazzi-Pearson, V., Modulation of host defence systems (2000) Arbuscular Mycorrhizas: Physiology and Function, pp. 173-199. , Edited by Y. Kapulnik and D.D. Douds. Kluwer Academic Publishers, Dordrecht
  • Franck, N., Producción y manejo de plantaciones de granado en Chile, Israel y Argentina (2009) Granados, Perspectivas Y Oportunidades De Un Negocio Emergente, pp. 28-35. , Edited by C. Castillo. Fundación Chile, Chile
  • Franco, J.A., Bañón, S., Vicente, M.J., Miralles, J., Martínez-Sánchez, J.J., Root development in horticultural plants grown under abiotic stress conditions - a review (2011) J. Hortic. Sci. Biotechnol, 86 (6), pp. 543-556
  • Giovanetti, M., Mosse, B., An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infectioninroots (1980) New Phytol, 84, pp. 489-500. , doi:10.1111/j.1469-8137.1980.tb04556.x
  • Gogorcena, Y., Iturbe-Ormaetxe, I., Escuredo, P.R., Becana, M., Antiox-idant defense against activated oxygen in pea nodules subjected to water stress (1995) Plant Physiol, 108, pp. 753-759. , PMID:12228507
  • Hewitt, E.J., Sand and water culture methodsin the studyof plant nutrition (1952) Tech. Com. Agric. Bur, p. 22
  • Hodges, D.M., Delong, J.M., Forney, C.F., Prange, R.K., Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxida-tion in plant tissues containing anthocyanin and other interfering compounds (1999) Planta, 207 (4), pp. 604-611. , doi:10.1007/s004250050524
  • Hossain, M.A., Asada, K., Inactivation of ascorbate peroxidase in spinach chloroplasts on dark addition of hydrogen peroxide: Its protection by ascorbate (1984) Plant Cell Physiol, 25 (7), pp. 1285-1295
  • Huang, L.L., Yang, C., Zhao, Y., Xu, X., Xu, Q., Li, G.Z., Cao, J., Hao, L., Antioxidant defenses of mycorrhizal fungus infection against SO2-induced oxidative stress in Avena nuda seedlings (2008) Bull. Environ. Cont. Toxicol, 81, pp. 440-444. , doi:10.1007/s00128-008-9521-7
  • Khattab, M.M., Shaban, A.E., El-Shrief, A.H., El-Deen Mohamed, A.S., Growth and productivity of pomegranate trees under different irrigation levels. I: Vegetative growth and fruiting (2011) J. Hortic. Sci. Ornam. Plants, 3 (2), pp. 194-198
  • Menge, J.A., Johnson, E.L.V., Platt, R.G., Mycorrhizal dependency of several citrus cultivars under three nutrient regimes (1978) New Phytol, 81 (3), pp. 553-559. , doi:10.1111/j.1469-8137.1978.tb01628.x
  • Miller, G., Suzuki, N., Ciftci-Yilmaz, S., Mittler, R., Reactive oxygen species homeostasis and signalling during drought and salinity stresses (2010) Plant Cell Environ, 33, pp. 453-467. , doi:10.1111/j.1365-3040.2009.02041.x. PMID: 19712065
  • Moran, J.F., Becana, M., Iturbe-Ormaetxe, I., Frechilla, S., Klucas, R.V., Aparicio-Tejo, P., Drought induces oxidative stress in pea plants (1994) Planta, 194 (3), pp. 346-352. , doi:10.1007/BF00197534
  • Nilsen, E.T., Orcutt, D.M., (1996) The Physiology of Plants Under Stress, 1. , John Wiley & Sons Inc., N.Y
  • Oelmüller, R., Sherameti, I., Tripathi, S., Varma, A., Piriformospora indica, a cultivable root endophyte with multiple biotechnological applications (2009) Symbiosis, 49, pp. 1-17. , doi:10.1007/s13199-009-0009-y
  • Parodi, L.R., (1978) Enciclopedia Argentina De Agricultura Y Jardinería, 2 (1). , Editorial ACME, Buenos Aires
  • Phillips, J.M., Hayman, D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection (1970) Trans. Brit. Mycol, 55, pp. 158-161. , doi:10.1016/S0007-1536(70)80110-3
  • Porcel, R., Ruíz-Lozano, J.M., Arbuscular mycorrhizal influence on leaf water potential, solute accumulation, and oxidative stress in soybean plants subjected to drought stress (2004) J. Exp. Bot, 55 (403), pp. 1743-1750. , doi:10.1093/jxb/ erh188. PMID:15208335
  • Porcel, R., Barea, J.M., Ruíz-Lozano, J.M., Antioxidant activities in my-corrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence (2003) New Phytol, 157, pp. 135-143. , doi:10.1046/j. 1469-8137.2003.00658.x
  • Rahmaty, R., Khara, J., Effects of vesicular arbuscular mycorrhiza Glomus intraradices on photosynthetic pigments, antioxidant enzymes, lipid peroxidation, and chromium accumulation in maize plants treated with chromium (2011) Turk. J. Biol, 35, pp. 51-58
  • Rizhsky, L., Liang, H., Mittler, R., The water-water cycle is essential for chloroplast protection in the absence of stress (2003) J. Biol. Chem, 278 (40), pp. 38921-38925. , doi:10.1074/jbc.M304987200. PMID:12885779
  • Roldán, A., Díaz-Vivancos, P., Hernández, J.A., Carrasco, L., Caravaca, F., Superoxide dismutase and total peroxidase activities in relation to drought recovery performance of mycorrhizal shrub seedlings grown in an amended semiarid soil (2008) J. Plant Physiol, 165, pp. 715-722. , doi:10.1016/j.jplph.2007. 02.007. PMID:17913291
  • Roqueiro, G., Maldonado, S., Ríos, M.D.C., Maroder, H., Fluctuation of oxidative stress indicators in Salix nigra seeds during priming (2012) J. Exp. Bot, 63 (10), pp. 3631-3642. , doi:10.1093/jxb/ers030
  • Ruíz-Lozano, J.M., Porcel, R., Azcón, C., Aroca, R., Regulation by arbus-cular mycorrhizae of the integrated physiological response to salinity in plants: New challenges in physiological and molecular studies (2012) J. Exp. Bot, 63 (11), pp. 4033-4044. , doi:10.1093/jxb/ers126. PMID:22553287
  • Saggin-Júnior, O.J., da Silva Ribeiro, E.M., Production of seedlings inoculated with arbuscular mycorrhizal fungi and their performance after outplanting (2005) Handbook of Microbial Biofertilizers, pp. 353-394. , Edited by M.K. Rai
  • Silvani, V.A., (2011) Aislamiento Y Caracterización In Vitro De Hongos Micorrícicos Arbusculares De Diferentes Sitios En Argentina, , Ph.D. thesis, Universidad de Buenos Aires
  • Stahl, P.D., Schuman, G.E., Frost, S.M., Williams, S.E., Arbuscular my-corrhizae and water stress tolerance of Wyoming big sagebrush seedling Soil Sci (1998) Soc. Am. J, 62, pp. 1309-1313. , doi:10.2136/sssaj1998.03615995006200050023x
  • Trappe, J.M., Phylogenetic and ecologic aspects of mycotrophy in angio-sperms from anevolutionary standpoint (1986) EcophysiologyofVA Mycorrhizal Plants, pp. 5-25. , Edited by G.R. Safir. CRC Press, Boca Raton, Fla
  • Waterer, D.R., Coltman, R.R., Response of mycorrhizal bell peppers to inoculation timing, phosphorus and water stress (1989) HortScience, 24, pp. 688-690
  • Wu, Q.S., Zou, Y.N., Mycorrhiza has a direct effect on reactive oxygen metabolism of drought-stressed citrus (2009) Plant Soil Environ, 55 (10), pp. 436-442
  • Wu, Q.S., Zou, Y.N., Xia, R.X., Effects of water stress and arbuscular mycorrhizal fungi on reactive oxygen metabolism and antioxidant production by citrus (Citrus tangerine) roots (2006) Eur. J. Soil Biol, 42, pp. 166-172. , doi:10.1016/ j.ejsobi.2005.12.006
  • Wu, Q.S., Zou, Y.N., Xia, R.X., Wang, M.Y., Five Glomus species affect water relations of Citrus tangerine during drought stress (2007) Bot. Stud, 48, pp. 147-154
  • Wu, Q.S., Xia, R.X., Zou, Y.N., Improved soil structure and citrus growth after inoculation with three arbuscular mycorrhizal fungi under drought stress (2008) Eur. J. Soil Biol, 44, pp. 122-128. , doi:10.1016/j.ejsobi.2007.10.001
  • Wu, Q.S., Zou, Y.N., Liu, W., Ye, X.F., Zai, H.F., Zao, L.J., Alleviation of salt stress in citrus seedlings inoculated with mycorrhiza: Changes in leaf antioxidant defense systems (2010) Plant Soil Environ, 56, pp. 470-475
  • Zhu, X., Song, F., Liu, S., Arbuscular mycorrhiza impacts on drought stress of maize plants by lipid peroxidation, proline content and activity of antioxidant system (2011) J. Food Agric. Environ, 9 (2), pp. 583-587

Citas:

---------- APA ----------
Bompadre, M.J., Silvani, V.A., Bidondo, L.F., Ríos de Molina, M.D.C., Colombo, R.P., Pardo, A.G. & Godeas, A.M. (2014) . Arbuscular mycorrhizal fungi alleviate oxidative stress in pomegranate plants growing under different irrigation conditions. Botany, 92(3), 187-193.
http://dx.doi.org/10.1139/cjb-2013-0169
---------- CHICAGO ----------
Bompadre, M.J., Silvani, V.A., Bidondo, L.F., Ríos de Molina, M.D.C., Colombo, R.P., Pardo, A.G., et al. "Arbuscular mycorrhizal fungi alleviate oxidative stress in pomegranate plants growing under different irrigation conditions" . Botany 92, no. 3 (2014) : 187-193.
http://dx.doi.org/10.1139/cjb-2013-0169
---------- MLA ----------
Bompadre, M.J., Silvani, V.A., Bidondo, L.F., Ríos de Molina, M.D.C., Colombo, R.P., Pardo, A.G., et al. "Arbuscular mycorrhizal fungi alleviate oxidative stress in pomegranate plants growing under different irrigation conditions" . Botany, vol. 92, no. 3, 2014, pp. 187-193.
http://dx.doi.org/10.1139/cjb-2013-0169
---------- VANCOUVER ----------
Bompadre, M.J., Silvani, V.A., Bidondo, L.F., Ríos de Molina, M.D.C., Colombo, R.P., Pardo, A.G., et al. Arbuscular mycorrhizal fungi alleviate oxidative stress in pomegranate plants growing under different irrigation conditions. Bot. 2014;92(3):187-193.
http://dx.doi.org/10.1139/cjb-2013-0169