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Abstract:

In an effort to gain an understanding of the processes governing matrix-assisted ultraviolet laser desorption/ionization (UV-MALDI), we made a direct comparison of the mass spectra (MS) of carbohydrates (neutral cyclic and acyclic, sulfated and acid mono- and polysaccharides) by using nor-harmane and several classical matrices (gentisic acid (GA); 2,4,6trihydroxixyacetophenone (THAP); among others) as UV-MALDI matrices in positive and negative ion modes in linear, reflectron and post-source decay (PSD) modes. The peculiarity of norharmane is that behaves better as matrix than GA in negative ion mode. Because the initiation of the UV-MALDI process is a photochemical reaction (matrix +hv (337 or 355 nm)→ matrix*), the acid-base character of nor-harmane in the ground and the lower electronic excited singlet state, together with other photophysical properties such as prompt heat release to the medium are discussed in brief. Excellent results obtained with cyclodextrins with nor-harmane allow us to propose these analyte-matrix systems for UV-MALDI-TOF MS calibration in positive and negative ion modes in linear, reflectron and PSD modes.

Registro:

Documento: Artículo
Título:UV-MALDI-TOF MS analysis of carbohydrates. reviewing comparative studies performed using nor-harmane and classical UV-MALDI matrices
Autor:Erra-Balsells, R.; Hiroshi Nonami
Filiación:CIHIDECAR (CONICET) - Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 30, Buenos Aires 1428, Argentina
Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan
Palabras clave:Carrageenan; Nor-harmane; Pectin; Polysaccharides; Uv-maldi matrix; UVMALDI-TOF MS; Xylan
Año:2008
Volumen:46
Número:2
Página de inicio:65
Página de fin:90
DOI: http://dx.doi.org/10.2525/ecb.46.65
Título revista:Environmental Control in Biology
Título revista abreviado:Enviro. Cont. Biol.
ISSN:1880554X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1880554X_v46_n2_p65_ErraBalsells

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Citas:

---------- APA ----------
Erra-Balsells, R. & Hiroshi Nonami (2008) . UV-MALDI-TOF MS analysis of carbohydrates. reviewing comparative studies performed using nor-harmane and classical UV-MALDI matrices. Environmental Control in Biology, 46(2), 65-90.
http://dx.doi.org/10.2525/ecb.46.65
---------- CHICAGO ----------
Erra-Balsells, R., Hiroshi Nonami "UV-MALDI-TOF MS analysis of carbohydrates. reviewing comparative studies performed using nor-harmane and classical UV-MALDI matrices" . Environmental Control in Biology 46, no. 2 (2008) : 65-90.
http://dx.doi.org/10.2525/ecb.46.65
---------- MLA ----------
Erra-Balsells, R., Hiroshi Nonami "UV-MALDI-TOF MS analysis of carbohydrates. reviewing comparative studies performed using nor-harmane and classical UV-MALDI matrices" . Environmental Control in Biology, vol. 46, no. 2, 2008, pp. 65-90.
http://dx.doi.org/10.2525/ecb.46.65
---------- VANCOUVER ----------
Erra-Balsells, R., Hiroshi Nonami UV-MALDI-TOF MS analysis of carbohydrates. reviewing comparative studies performed using nor-harmane and classical UV-MALDI matrices. Enviro. Cont. Biol. 2008;46(2):65-90.
http://dx.doi.org/10.2525/ecb.46.65