Artículo

Cerezo, A.S.; Giudicessi, S.L.; Erra-Balsells, R.; Sato, Y.; Nonami, H.; Marquinez, A.C.; Wolfenstein-Todel, C.; De Scacciati Cerezo, J.M. "Direct UV-MALDI-TOF MS analysis of (Glyco)proteins of fractions of bovine seminal plasma" (2007) Environmental Control in Biology. 45(4):267-290
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Abstract:

Bovine seminal plasma was submitted to chromatography on Con A-Sepharose. The "noninteracting", "weakly-interacting" and "strongly-interacting" fractions were analyzed through UV-MALDI-TOF MS together with a subfraction of the "non-interacting" material, using sinapinic acid (SA) as matrix. The spectra were obtained in linear positive mode in the 4.0-90.0 kDa mass/charge range showing peaks in well defined zones, namely: 5.5-8.0 kDa, 10.0-12.0 kDa, 12.5-14.0 kDa (major), 23.2-23.7 kDa, 26.1-27.5 kDa and 38.0-40.0 kDa. High sensitivity spectra showed some very small peaks until 90 kDa. Bovine seminal protein (BSP-A3), acidic seminal fluid protein (aSFP) and PDC-109 glycoproteins (BSP-A1 and BSP-A2) were identified. Caltrin, the human epididymis-specific glycoprotein (HE4), the calcium transport inhibitor protein, the inhibitor of metalloprotease 2 (TIMP-2), osteopontin (OPN) and the prostatic acid protease (PAP) were tentatively identified. The molecular weight of some peaks can be arranged in a sequence from that of BSP-A3 going through the molecular weights of glycoforms (including the known BSP-A1 and BSP-A2) which differ in the amounts of neutral hexoses and sialic acids, composing a BSP-family more extended than previously reported. Another two families could be builded up from proteins of molecular weight of about 12730 and 12750 Da and glycoforms which differ from them also by hexoses and sialic acids. The structures of the deduced O-linked oligosaccharides of the glycoforms are in complete agreement to that determined for the BSP-A1 Oligosaccharide. Small differences in the m. w. of some (glyco)proteins were attributed to genetic polymorphysm. The identification of proteins and O-linked glycoproteins in the "interacting" fractions of the chromatography suggests that the fractionation was not due to specific affinity interactions but to non-specific hydrophobic interactions of the proteins with the hydrophobic pocked of the Con A.

Registro:

Documento: Artículo
Título:Direct UV-MALDI-TOF MS analysis of (Glyco)proteins of fractions of bovine seminal plasma
Autor:Cerezo, A.S.; Giudicessi, S.L.; Erra-Balsells, R.; Sato, Y.; Nonami, H.; Marquinez, A.C.; Wolfenstein-Todel, C.; De Scacciati Cerezo, J.M.
Filiación:CIHIDECAR (CONICET) - Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Buenos Aires 1428, Argentina
United Graduate School of Agricultural Sciences, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan
Plant Biophysics/Biochemistry Research Laboratory, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan
Centro de Investigaciones en Reproduccion (CIR), Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires 1114, Argentina
Instituto de Química y Fisicoquimica Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires 1114, Argentina
Palabras clave:Bovine seminal plasma; Glyco)proteins; Nor-harmane; Sinapinic acid; UV-MALDITOF MS
Año:2007
Volumen:45
Número:4
Página de inicio:267
Página de fin:290
DOI: http://dx.doi.org/10.2525/ecb.45.267
Título revista:Environmental Control in Biology
Título revista abreviado:Enviro. Cont. Biol.
ISSN:1880554X
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_1880554X_v45_n4_p267_Cerezo.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1880554X_v45_n4_p267_Cerezo

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Citas:

---------- APA ----------
Cerezo, A.S., Giudicessi, S.L., Erra-Balsells, R., Sato, Y., Nonami, H., Marquinez, A.C., Wolfenstein-Todel, C.,..., De Scacciati Cerezo, J.M. (2007) . Direct UV-MALDI-TOF MS analysis of (Glyco)proteins of fractions of bovine seminal plasma. Environmental Control in Biology, 45(4), 267-290.
http://dx.doi.org/10.2525/ecb.45.267
---------- CHICAGO ----------
Cerezo, A.S., Giudicessi, S.L., Erra-Balsells, R., Sato, Y., Nonami, H., Marquinez, A.C., et al. "Direct UV-MALDI-TOF MS analysis of (Glyco)proteins of fractions of bovine seminal plasma" . Environmental Control in Biology 45, no. 4 (2007) : 267-290.
http://dx.doi.org/10.2525/ecb.45.267
---------- MLA ----------
Cerezo, A.S., Giudicessi, S.L., Erra-Balsells, R., Sato, Y., Nonami, H., Marquinez, A.C., et al. "Direct UV-MALDI-TOF MS analysis of (Glyco)proteins of fractions of bovine seminal plasma" . Environmental Control in Biology, vol. 45, no. 4, 2007, pp. 267-290.
http://dx.doi.org/10.2525/ecb.45.267
---------- VANCOUVER ----------
Cerezo, A.S., Giudicessi, S.L., Erra-Balsells, R., Sato, Y., Nonami, H., Marquinez, A.C., et al. Direct UV-MALDI-TOF MS analysis of (Glyco)proteins of fractions of bovine seminal plasma. Enviro. Cont. Biol. 2007;45(4):267-290.
http://dx.doi.org/10.2525/ecb.45.267