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Abstract:

Protein kinase A (PKA) activity is involved in dimorphism of the basal fungal lineage Mucor. From the recently sequenced genome of Mucor circinelloides we could predict ten catalytic subunits of PKA. From sequence alignment and structural prediction we conclude that the catalytic core of the isoforms is conserved, and the difference between them resides in their amino termini. This high number of isoforms is maintained in the subdivision Mucoromycotina. Each paralogue, when compared to the ones form other fungi is more homologous to one of its orthologs than to its paralogs. All of these fungal isoforms cannot be included in the class I or II in which fungal protein kinases have been classified. mRNA levels for each isoform were measured during aerobic and anaerobic growth. The expression of each isoform is differential and associated to a particular growth stage. We reanalyzed the sequence of PKAC (GI 20218944), the only cloned sequence available until now for a catalytic subunit of M. circinelloides. PKAC cannot be classified as a PKA because of its difference in the conserved C-tail; it shares with PKB a conserved C2 domain in the N-terminus. No catalytic activity could be measured for this protein nor predicted bioinformatically. It can thus be classified as a pseudokinase. Its importance can not be underestimated since it is expressed at the mRNA level in different stages of growth, and its deletion is lethal. © 2016 British Mycological Society

Registro:

Documento: Artículo
Título:Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides
Autor:Fernández Núñez, L.; Ocampo, J.; Gottlieb, A.M.; Rossi, S.; Moreno, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN-CONICET, Intendente Güiraldes 2160 – Ciudad Universitaria – C1428EGA, Buenos Aires, Argentina
Departamento de Ecologia, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IEGEBA-CONICET, Intendente Güiraldes 2160 – Ciudad Universitaria – C1428EGA, Buenos Aires, Argentina
Division of Developmental Biology, Eunice Kennedy Shriver National Institute for Child Health and Human Development, National Institutes of Health, Bethesda, United States
Palabras clave:cAMP; Differentiation; Orthologs; Paralogs; Pseudokinase; Zygomycetes; biochemistry; catalysis; chemical property; cladistics; dimorphism; enzyme activity; fungus; gene expression; genome; growth; protein; Fungi; Mucor; Mucor circinelloides; Zygomycetes; cyclic AMP dependent protein kinase; isoprotein; messenger RNA; aerobic metabolism; anaerobic growth; enzyme active site; enzymology; gene expression profiling; genetics; growth, development and aging; Mucor; sequence homology; Aerobiosis; Anaerobiosis; Catalytic Domain; Cyclic AMP-Dependent Protein Kinases; Gene Expression Profiling; Mucor; Protein Isoforms; RNA, Messenger; Sequence Homology
Año:2016
Volumen:120
Número:12
Página de inicio:1493
Página de fin:1508
DOI: http://dx.doi.org/10.1016/j.funbio.2016.07.013
Título revista:Fungal Biology
Título revista abreviado:Fungal Biol.
ISSN:18786146
CAS:cyclic AMP dependent protein kinase; Cyclic AMP-Dependent Protein Kinases; Protein Isoforms; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18786146_v120_n12_p1493_FernandezNunez

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Citas:

---------- APA ----------
Fernández Núñez, L., Ocampo, J., Gottlieb, A.M., Rossi, S. & Moreno, S. (2016) . Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides. Fungal Biology, 120(12), 1493-1508.
http://dx.doi.org/10.1016/j.funbio.2016.07.013
---------- CHICAGO ----------
Fernández Núñez, L., Ocampo, J., Gottlieb, A.M., Rossi, S., Moreno, S. "Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides" . Fungal Biology 120, no. 12 (2016) : 1493-1508.
http://dx.doi.org/10.1016/j.funbio.2016.07.013
---------- MLA ----------
Fernández Núñez, L., Ocampo, J., Gottlieb, A.M., Rossi, S., Moreno, S. "Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides" . Fungal Biology, vol. 120, no. 12, 2016, pp. 1493-1508.
http://dx.doi.org/10.1016/j.funbio.2016.07.013
---------- VANCOUVER ----------
Fernández Núñez, L., Ocampo, J., Gottlieb, A.M., Rossi, S., Moreno, S. Multiple isoforms for the catalytic subunit of PKA in the basal fungal lineage Mucor circinelloides. Fungal Biol. 2016;120(12):1493-1508.
http://dx.doi.org/10.1016/j.funbio.2016.07.013