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Abstract:

Establishment of arbuscular mycorrhizal (AM) germplasm collections is complex because of the obligate biotrophic nature of AM fungi. Only a few AM species are routinely maintained in monoxenic culture with Ri T-DNA transformed roots as host. Incorporation of new AM species into this culture system is important for molecular, physiological, and taxonomical studies. Here we report for the first time the successful monoxenic culture of Gigaspora decipiens (JA2 strain) with transformed carrot (Daucus carota) roots. In vitro cultures were established from field-collected spores; sub-culture of newly in vitro formed spores was established over five successive generations for a period of 6 y. Although initial culture of field-collected spores was difficult successive sub-cultures appeared to be adapted to the in vitro growing conditions. The JA2 strain of G. decipiens completed its life cycle while maintaining its morphological characteristics, stability, and propagule viability under the monoxenic conditions over several generations. This stable and homogeneous monoxenic material obtained for G. decipiens is part of the Banco de Glomeromycota In Vitro (BGIV, http://www.bgiv.com.ar), and could facilitate morphological, physiological, and molecular analysis of this AM species. © 2012 British Mycological Society.

Registro:

Documento: Artículo
Título:Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture
Autor:Bidondo, L.F.; Pergola, M.; Silvani, V.; Colombo, R.; Bompadre, J.; Godeas, A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to Piso, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Five successive generations of axenic spores; Gigaspora decipiens; fungal DNA; arbuscular mycorrhiza; dicotyledon; fungus; germplasm; host; molecular analysis; physiological response; physiology; propagule; root; spore; article; carrot; chemistry; DNA sequence; fungus spore; genetics; Glomeromycota; growth, development and aging; isolation and purification; methodology; microbial viability; microbiology; molecular genetics; mycology; nucleotide sequence; plant root; Daucus carota; DNA, Fungal; Glomeromycota; Microbial Viability; Molecular Sequence Data; Mycology; Plant Roots; Sequence Analysis, DNA; Spores, Fungal; Arbuscular; Bacteria (microorganisms); Daucus carota; Fungi; Gigaspora decipiens; Glomeromycota
Año:2012
Volumen:116
Número:6
Página de inicio:729
Página de fin:735
DOI: http://dx.doi.org/10.1016/j.funbio.2012.04.007
Título revista:Fungal Biology
Título revista abreviado:Fungal Biol.
ISSN:18786146
CAS:DNA, Fungal
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18786146_v116_n6_p729_Bidondo

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Citas:

---------- APA ----------
Bidondo, L.F., Pergola, M., Silvani, V., Colombo, R., Bompadre, J. & Godeas, A. (2012) . Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture. Fungal Biology, 116(6), 729-735.
http://dx.doi.org/10.1016/j.funbio.2012.04.007
---------- CHICAGO ----------
Bidondo, L.F., Pergola, M., Silvani, V., Colombo, R., Bompadre, J., Godeas, A. "Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture" . Fungal Biology 116, no. 6 (2012) : 729-735.
http://dx.doi.org/10.1016/j.funbio.2012.04.007
---------- MLA ----------
Bidondo, L.F., Pergola, M., Silvani, V., Colombo, R., Bompadre, J., Godeas, A. "Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture" . Fungal Biology, vol. 116, no. 6, 2012, pp. 729-735.
http://dx.doi.org/10.1016/j.funbio.2012.04.007
---------- VANCOUVER ----------
Bidondo, L.F., Pergola, M., Silvani, V., Colombo, R., Bompadre, J., Godeas, A. Continuous and long-term monoxenic culture of the arbuscular mycorrhizal fungus Gigaspora decipiens in root organ culture. Fungal Biol. 2012;116(6):729-735.
http://dx.doi.org/10.1016/j.funbio.2012.04.007