Artículo
Questa, M.; Romorini, L.; Blüguermann, C.; Solari, C.M.; Neiman, G.; Luzzani, C.; Scassa, M.T.; Sevlever, G.E.; Guberman, A.S.; Miriuka, S.G. "Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts" (2016) Stem Cell Research. 16(2):300-303
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Abstract:
Human foreskin fibroblasts were used to generate the iPSC line iPSC-FH2.1 using the EF1a-hSTEMCCA-loxP vector expressing OCT4, SOX2, c-MYC and KLF4, in 5% O2 culture conditions. Stemness was confirmed, as was pluripotency both in vivo and in vitro, in normoxia and hypoxia. Human Embryonic Stem Cell (hESC) line WA-09 and reprogrammed fibroblast primary culture HFF-FM were used as controls. © 2015 The Authors.
Registro:
| Documento: | Artículo |
| Título: | Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts |
| Autor: | Questa, M.; Romorini, L.; Blüguermann, C.; Solari, C.M.; Neiman, G.; Luzzani, C.; Scassa, M.T.; Sevlever, G.E.; Guberman, A.S.; Miriuka, S.G. |
| Filiación: | LIAN-CONICET, FLENI, Ruta 9 Km 52.5, Belén de Escobar, Buenos Aires, B1625XAF, Argentina Laboratorio de Regulación de la Expresión Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, IQUIBICEN, CONICET-UBA, Intendente Güiraldes 2160, Ciudad Universitaria, Capital Federal, Buenos Aires, C1428EGA, Argentina Carrera de Investigador, CONICET, Argentina Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
| Palabras clave: | kruppel like factor 4; Myc protein; octamer transcription factor 4; transcription factor Sox2; octamer transcription factor 4; POU5F1 protein, human; transcription factor; Article; cell differentiation; controlled study; CpG island; fibroblast; gene expression; human; human cell; human embryonic stem cell; hypoxic cell; in vitro study; in vivo study; nuclear reprogramming; pluripotent stem cell; prepuce; priority journal; stem cell line; cell culture; comparative genomic hybridization; cytology; DNA methylation; fibroblast; fluorescence microscopy; genetics; induced pluripotent stem cell; karyotype; male; metabolism; prepuce; promoter region; real time polymerase chain reaction; Cell Differentiation; Cells, Cultured; Cellular Reprogramming; Comparative Genomic Hybridization; DNA Methylation; Fibroblasts; Foreskin; Humans; Induced Pluripotent Stem Cells; Karyotype; Male; Microscopy, Fluorescence; Octamer Transcription Factor-3; Promoter Regions, Genetic; Real-Time Polymerase Chain Reaction; Transcription Factors |
| Año: | 2016 |
| Volumen: | 16 |
| Número: | 2 |
| Página de inicio: | 300 |
| Página de fin: | 303 |
| DOI: | http://dx.doi.org/10.1016/j.scr.2015.12.026 |
| Título revista: | Stem Cell Research |
| Título revista abreviado: | Stem Cell Res. |
| ISSN: | 18735061 |
| CAS: | Octamer Transcription Factor-3; POU5F1 protein, human; Transcription Factors |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18735061_v16_n2_p300_Questa |
Referencias:
- Luzzani, C., Neiman, G., Garate, X., Questa, M., Solari, C., A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement (2015) Stem Cell Res. Ther., 6, p. 6
- Meng, G., Liu, S., Krawetz, R., Chan, M., Chernos, J., A novel method for generating xeno-free human feeder cells for human embryonic stem cell culture (2008) Stem Cells Dev., 17, pp. 413-422
- Romorini, L., Scassa, M.E., Videla Richardson, G., Bluguermann, C., Jaquenod de Giusti, C., Activation of apoptotic signalling events in human embryonic stem cells upon Coxsackievirus B3 infection (2012) Apoptosis, 17, pp. 132-142
- Scassa, M.E., Jaquenod de Giusti, C., Questa, M., Pretre, G., Richardson, G.A., Human embryonic stem cells and derived contractile embryoid bodies are susceptible to Coxsakievirus B infection and respond to interferon Ibeta treatment (2011) Stem Cell Res., 6, pp. 13-22
- Sommer, C.A., Stadtfeld, M., Murphy, G.J., Hochedlinger, K., Kotton, D.N., Induced pluripotent stem cell generation using a single lentiviral stem cell cassette (2009) Stem Cells, 27, pp. 543-549
Citas:
---------- APA ----------
Questa, M., Romorini, L., Blüguermann, C., Solari, C.M., Neiman, G., Luzzani, C., Scassa, M.T.,..., Miriuka, S.G. (2016) . Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts. Stem Cell Research, 16(2), 300-303.http://dx.doi.org/10.1016/j.scr.2015.12.026
---------- CHICAGO ----------
Questa, M., Romorini, L., Blüguermann, C., Solari, C.M., Neiman, G., Luzzani, C., et al. "Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts" . Stem Cell Research 16, no. 2 (2016) : 300-303.http://dx.doi.org/10.1016/j.scr.2015.12.026
---------- MLA ----------
Questa, M., Romorini, L., Blüguermann, C., Solari, C.M., Neiman, G., Luzzani, C., et al. "Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts" . Stem Cell Research, vol. 16, no. 2, 2016, pp. 300-303.http://dx.doi.org/10.1016/j.scr.2015.12.026
---------- VANCOUVER ----------
Questa, M., Romorini, L., Blüguermann, C., Solari, C.M., Neiman, G., Luzzani, C., et al. Generation of iPSC line iPSC-FH2.1 in hypoxic conditions from human foreskin fibroblasts. Stem Cell Res. 2016;16(2):300-303.http://dx.doi.org/10.1016/j.scr.2015.12.026
