Abstract:
Leptin is an adipose-derived hormone that controls appetite and energy expenditure. Leptin receptors are expressed on extra-hypothalamic ventrobasal (VB) and reticular thalamic (RTN) nuclei from embryonic stages. Here, we studied the effects of pressure-puff, local application of leptin on both synaptic transmission and action potential properties of thalamic neurons in thalamocortical slices. We used whole-cell patch-clamp recordings of thalamocortical VB neurons from wild-type (WT) and leptin-deficient obese (ob/ob) mice. We observed differences in VB neurons action potentials and synaptic currents kinetics when comparing WT vs. ob/ob. Leptin reduced GABA release onto VB neurons throughout the activation of a JAK2-dependent pathway, without affecting excitatory glutamate transmission. We observed a rapid and reversible reduction by leptin of the number of action potentials of VB neurons via the activation of large conductance Ca 2+ -dependent potassium channels. These leptin effects were observed in thalamocortical slices from up to 5-week-old WT but not in leptin-deficient obese mice. Results described here suggest the existence of a leptin-mediated trophic modulation of thalamocortical excitability during postnatal development. These findings could contribute to a better understanding of leptin within the thalamocortical system and sleep deficits in obesity. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.
Registro:
Documento: |
Artículo
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Título: | Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons |
Autor: | Perissinotti, P.P.; Rivero-Echeto, M.C.; Garcia-Rill, E.; Bisagno, V.; Urbano, F.J. |
Filiación: | Departamento de Fisiología, Facultad de Ciencias Exactas y Naturales, Biología Molecular y Celular “Dr. Héctor Maldonado”, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina Instituto de Investigaciones Farmacológicas (ININFA), CONICET-Universidad de Buenos Aires, Ciudad de Buenos Aires, Argentina Department of Neurobiology and Developmental Sciences, Center for Translational Neuroscience, University of Arkansas for Medical Sciences, Little Rock, AR, United States
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Palabras clave: | GABA; Leptin; Mouse; ob/ob; Thalamic reticular nucleus; 4 aminobutyric acid; calcium activated potassium channel; glutamic acid; Janus kinase 2; leptin; recombinant leptin; 4 aminobutyric acid; 6 cyano 7 nitro 2,3 quinoxalinedione; alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; amino acid receptor blocking agent; enzyme inhibitor; Jak2 protein, mouse; Janus kinase 2; leptin; sodium channel blocking agent; tetrodotoxin; tyrphostin; 4 aminobutyric acid release; action potential; animal experiment; animal tissue; Article; brain slice; controlled study; GABAergic transmission; glutamatergic synapse; male; mouse; nerve excitability; neuromodulation; nonhuman; obesity; priority journal; synaptic transmission; thalamus reticular nucleus; thalamus ventral nucleus; whole cell patch clamp; animal; body temperature; C57BL mouse; cytology; deficiency; drug effect; genetics; metabolism; nerve cell; physiology; signal transduction; synaptic potential; thalamus nucleus; transgenic mouse; 6-Cyano-7-nitroquinoxaline-2,3-dione; Action Potentials; Animals; Body Temperature; Enzyme Inhibitors; Excitatory Amino Acid Antagonists; gamma-Aminobutyric Acid; Janus Kinase 2; Leptin; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Neurons; Signal Transduction; Sodium Channel Blockers; Synaptic Potentials; Synaptic Transmission; Tetrodotoxin; Thalamic Nuclei; Tyrphostins |
Año: | 2018
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Volumen: | 223
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Número: | 5
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Página de inicio: | 2499
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Página de fin: | 2514
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DOI: |
http://dx.doi.org/10.1007/s00429-018-1645-x |
Título revista: | Brain Structure and Function
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Título revista abreviado: | Brain Struct. Funct.
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ISSN: | 18632653
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CAS: | 4 aminobutyric acid, 28805-76-7, 56-12-2; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; 6 cyano 7 nitro 2,3 quinoxalinedione, 115066-14-3; tetrodotoxin, 4368-28-9, 4664-41-9; 6-Cyano-7-nitroquinoxaline-2,3-dione; alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; Enzyme Inhibitors; Excitatory Amino Acid Antagonists; gamma-Aminobutyric Acid; Jak2 protein, mouse; Janus Kinase 2; Leptin; Sodium Channel Blockers; Tetrodotoxin; Tyrphostins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18632653_v223_n5_p2499_Perissinotti |
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Citas:
---------- APA ----------
Perissinotti, P.P., Rivero-Echeto, M.C., Garcia-Rill, E., Bisagno, V. & Urbano, F.J.
(2018)
. Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons. Brain Structure and Function, 223(5), 2499-2514.
http://dx.doi.org/10.1007/s00429-018-1645-x---------- CHICAGO ----------
Perissinotti, P.P., Rivero-Echeto, M.C., Garcia-Rill, E., Bisagno, V., Urbano, F.J.
"Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons"
. Brain Structure and Function 223, no. 5
(2018) : 2499-2514.
http://dx.doi.org/10.1007/s00429-018-1645-x---------- MLA ----------
Perissinotti, P.P., Rivero-Echeto, M.C., Garcia-Rill, E., Bisagno, V., Urbano, F.J.
"Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons"
. Brain Structure and Function, vol. 223, no. 5, 2018, pp. 2499-2514.
http://dx.doi.org/10.1007/s00429-018-1645-x---------- VANCOUVER ----------
Perissinotti, P.P., Rivero-Echeto, M.C., Garcia-Rill, E., Bisagno, V., Urbano, F.J. Leptin alters somatosensory thalamic networks by decreasing gaba release from reticular thalamic nucleus and action potential frequency at ventrobasal neurons. Brain Struct. Funct. 2018;223(5):2499-2514.
http://dx.doi.org/10.1007/s00429-018-1645-x