Artículo
Abstract:
In this work, we present an alternative imaging method based on the orbital scanning of a laser excitation beam around the object of interest. This technology is capable of producing tridimensional images of fluorescent structures with nanometrical resolution in a few milliseconds. The method relies on the fact that when the excitation beam is near a fluorescent object, the emitted light from the object depends on the distance between its surface and the excitation beam. By modulating the distance between the beam and the object and taking into account the nonlinearity of the excitation intensity profile, it is possible to obtain an oscillating response whose amplitude depends only on the distance to the surface of the object. Given the fact that the excitation beam is always near the structure of interest, it is possible to measure moving specimens. Here, we present tridimensional reconstructions of Arabidopsis Thaliana roots which are ~ 50 μm in length and ~ 5 μm in diameter. © 2018, Centro de Investigaciones en Laseres y Aplicaciones. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | 3D microscopy by orbital radially modulated scan |
| Autor: | Zaza, C.; Gabriel, M.; Estrada, L.C. |
| Filiación: | Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina IFIBA-CONICET, Ciudad UniversitariaBuenos Aires 1428, Argentina |
| Palabras clave: | Fluorescence; Microscopy; Single particle tracking |
| Año: | 2018 |
| Volumen: | 29 |
| Número: | 1 |
| Página de inicio: | 12 |
| Página de fin: | 19 |
| DOI: | http://dx.doi.org/10.31527/analesafa.2018.29.1.12 |
| Título revista: | Anales de la Asociacion Fisica Argentina |
| Título revista abreviado: | An. Asoc. Fis. Argent. |
| ISSN: | 18501168 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_18501168_v29_n1_p12_Zaza |
Referencias:
- Bissell, M.J., Radisky, D.C., Rizki, A., Weaver, V.M., Petersen, O.W., The organizing principle: Microenvironmental influences in the normal and malignant breast (2000) Differentiation, 70 (9-10), pp. 537-546
- Pampaloni, F., Reynaud, E.G., Stelzer, E.H.K., The third dimension bridges the gap between cell culture and live tissue (2007) Nature Reviews, 8 (10), pp. 839-845
- Chiu, C., (2013) 3D Cell-Ecm Dynamics Revealed by Innovative Fluorescence Microscopy Methods, , Tesis de doctorado en ciencias biológicas, Universidad de California
- Edmondson, R., Broglie, J.J., Adcock, A.F., Yang, L., Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors (2014) Assay and Drug Development Technologies, 12 (4), pp. 207-218
- Fang, Y., Eglen, R.M., Three-Dimensional Cell Cultures in Drug Discovery and Development (2017) Slas Discovery., 22 (5), pp. 456-472. , Eglen RM, Klein J-L, eds
- Whitehead, L., McArthur, K., Geoghegan, N., Rogers, K., The reinvention of twentieth century microscopy for three-dimensional imaging (2017) Immunology and Cell Biology, 95, pp. 520-524
- Kis-Petikova, K., Gratton, E., Distance mesurement by circular scanning of the excitation beam in the two photon microscope (2004) Microsc. Res. Tech., 63, pp. 34-49
- Lanzano, L., Digman, M.A., Fwu, P., Giral, H., Levi, M., Gratton, E., Nanometer scale imaging by the modulation tracking method (2011) J. Biophotonics, 4 (6), pp. 415-424
- Lanzano, L., Gratton, E., Measurement of distance with the nanoscale precise imaging rapid beam oscillation method (2012) Microsc. Res. Tech., 75 (9), pp. 1253-1264
- Levi, V., Ruan, Q., Gratton, E., 3-D Particle Tracking in a Two Photon Microscope: Application to the Study of Molecular Dynamics in Cells (2005) Biophysical Journal, 88 (4), pp. 2919-2928
- Gabriel, M., Moya-Díaz, J., Gallo, L.I., Marengo, F.D., Estrada, L.C., Single particle tracking of internalized metallic nanoparticles reveals heterogeneous directed motion after clathrin dependent endocytosis in mouse chromaffin cells (2018) Methods Appl Fluoresc, 6 (1)
Citas:
---------- APA ----------
Zaza, C., Gabriel, M. & Estrada, L.C. (2018) . 3D microscopy by orbital radially modulated scan . Anales de la Asociacion Fisica Argentina, 29(1), 12-19.http://dx.doi.org/10.31527/analesafa.2018.29.1.12
---------- CHICAGO ----------
Zaza, C., Gabriel, M., Estrada, L.C. "3D microscopy by orbital radially modulated scan " . Anales de la Asociacion Fisica Argentina 29, no. 1 (2018) : 12-19.http://dx.doi.org/10.31527/analesafa.2018.29.1.12
---------- MLA ----------
Zaza, C., Gabriel, M., Estrada, L.C. "3D microscopy by orbital radially modulated scan " . Anales de la Asociacion Fisica Argentina, vol. 29, no. 1, 2018, pp. 12-19.http://dx.doi.org/10.31527/analesafa.2018.29.1.12
---------- VANCOUVER ----------
Zaza, C., Gabriel, M., Estrada, L.C. 3D microscopy by orbital radially modulated scan . An. Asoc. Fis. Argent. 2018;29(1):12-19.http://dx.doi.org/10.31527/analesafa.2018.29.1.12
