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Abstract:

Previously, we studied the erythroblastic leukemia viral oncogene homolog (erbB) family of tyrosine kinase growth factor receptors in terms of protein expression, modulation and activation in the 3T3-L1 cell line. In the present study, the presence of full-length erbB mRNAs, and splice or proteolytic erbB variants, was evaluated using RT-PCR. Epidermal growth factor receptor (EGFR)/erbB1 expression was confirmed. Wild-type (wt) ErbB2, human erbB2 (HER2)-extracellular domain (ECD) and herstatin mRNA expression were analyzed. Restriction analysis confirmed wt expression. 3T3-L1 cells exhibited HER2-ECD and herstatin mRNA expression, although at extremely low levels (compared to the control cell lines). ErbB3 cDNA was amplified using mouse mammary tumors and rat acines as positive controls. ErbB4 was not positively identified in these cells. In conclusion, this study demonstrates that 3T3-L1 cells express EGFR/erbB1, erbB2 and erbB3, and possibly, certain erbB2 splice or proteolytic variants, which are worth pursuing.

Registro:

Documento: Artículo
Título:Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes
Autor:Pagano, E.; Fontana, V.; Calvo, J.C.
Filiación:Laboratorio de Química de Proteoglicanos Y Matriz Extracelular, IByME, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Biología Celular Y Molecular, PIB-UCA-CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina
Laboratorio de Biología Celular Y Molecular, Programa de Investigaciones Biomédicas, Pontificia Universidad Católica Argentina (PIB-UCA-CONICET), Alicia Moreau de Justo 1600, (C1107AFF), Buenos Aires, Argentina
Facultad de Ciencias Médicas, Pontificia Universidad Católica Argentina, Alicia Moreau de Justo 1500, (C1107AFF), Buenos Aires, Argentina
Palabras clave:3T3-L1 cells; erbB2; erbB3; erbB4; Erythroblastic leukemia viral oncogene homolog (erbB) 1/epidermal growth factor receptor; Heregulin isoform 2-extracellular domain; Herstatin; Preadipocytes; complementary DNA; epidermal growth factor receptor; epidermal growth factor receptor 2; epidermal growth factor receptor 3; epidermal growth factor receptor 4; messenger RNA; animal cell; article; cell strain 3T3; mouse; nonhuman; nucleotide sequence; proadipocyte; protein analysis; protein expression; 3T3-L1 Cells; Adipocytes; Alternative Splicing; Animals; Cell Proliferation; Gene Expression Regulation; Humans; Intercellular Signaling Peptides and Proteins; Mice; Rats; Receptor, Epidermal Growth Factor; Receptor, erbB-2; Receptor, erbB-3; Receptors, Growth Factor; RNA, Messenger; Rattus
Año:2011
Volumen:4
Número:5
Página de inicio:955
Página de fin:961
DOI: http://dx.doi.org/10.3892/mmr.2011.517
Título revista:Molecular Medicine Reports
Título revista abreviado:Mol. Med. Rep.
ISSN:17912997
CAS:epidermal growth factor receptor 2, 137632-09-8; epidermal growth factor receptor 3, 497121-54-7; ERBB4 protein, 2.7.1.112; Intercellular Signaling Peptides and Proteins; RNA, Messenger; Receptor, Epidermal Growth Factor, 2.7.10.1; Receptor, erbB-2, 2.7.10.1; Receptor, erbB-3, 2.7.10.1; Receptors, Growth Factor; herstatin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17912997_v4_n5_p955_Pagano

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Citas:

---------- APA ----------
Pagano, E., Fontana, V. & Calvo, J.C. (2011) . Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes. Molecular Medicine Reports, 4(5), 955-961.
http://dx.doi.org/10.3892/mmr.2011.517
---------- CHICAGO ----------
Pagano, E., Fontana, V., Calvo, J.C. "Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes" . Molecular Medicine Reports 4, no. 5 (2011) : 955-961.
http://dx.doi.org/10.3892/mmr.2011.517
---------- MLA ----------
Pagano, E., Fontana, V., Calvo, J.C. "Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes" . Molecular Medicine Reports, vol. 4, no. 5, 2011, pp. 955-961.
http://dx.doi.org/10.3892/mmr.2011.517
---------- VANCOUVER ----------
Pagano, E., Fontana, V., Calvo, J.C. Expression of erythroblastic leukemia viral oncogene homolog (erbBS) mRNAs and possible splice variants in 3T3-L1 preadipocytes. Mol. Med. Rep. 2011;4(5):955-961.
http://dx.doi.org/10.3892/mmr.2011.517