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Abstract:

Since the introduction of 3,5-dimethoxy-4-hydroxycinnamic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, the successful application of matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) started. Cinnamics can exist as two different geometric isomers, the E- and Z-forms. The commercially available cinnamics currently used as matrices are E-cinnamics; they do not perform well in general for carbohydrate analysis. Recently, Z-cinnamic acid properties for matrices were studied and compared with those of the corresponding E-isomer. For the analysis of neutral/sulfated carbohydrates the outstanding performance for Z-SA was demonstrated. As the synthesis of pure Z-cinnamic acids requires several steps and the manipulation of some not friendly chemicals (i.e., bad smelling organic amines, toxic compounds, organic solvents, etc.), here we describe a convenient new one-pot protocol to prepare in situ, in a methanolic solution of commercial E-acid (i.e., E-SA), a Z- + E- mixture by photoisomerization (UVB irradiation); then, the only step required is the addition of water to the irradiated solution to become ready as a matrix stock solution for MALDI experiments. This "photo-made at home" matrix performs carbohydrate analysis with similar results to the corresponding Z-acid. The results here show that this novel protocol is a tool of choice for the direct, rapid and sensitive detection of neutral and sulfated carbohydrates without any tedious Z-cinnamic acid preparation and isolation. © 2015 The Royal Society of Chemistry.

Registro:

Documento: Artículo
Título:Easy protocol for making a good E-sinapinic acid matrix for neutral and sulfated carbohydrate MALDI-MS analysis
Autor:Salum, M.L.; Schmidt De León, T.; Erra-Balsells, R.
Filiación:CIHIDECAR-Departamento de Química Orgánica, FCEN, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 3er P, Buenos Aires, 1428, Argentina
Palabras clave:Desorption; Isomers; Mass spectrometry; Organic chemicals; Plants (botany); Carbohydrate analysis; Cyano-4-hydroxycinnamic acids; Geometric isomer; Irradiated solutions; Matrix-assisted laser desorption/ionization mass spectrometry; Methanolic solution; One-pot protocol; Sensitive detection; Carbohydrates
Año:2015
Volumen:7
Número:19
Página de inicio:8478
Página de fin:8483
DOI: http://dx.doi.org/10.1039/c5ay01484k
Título revista:Analytical Methods
Título revista abreviado:Anal. Methods
ISSN:17599660
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17599660_v7_n19_p8478_Salum

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Citas:

---------- APA ----------
Salum, M.L., Schmidt De León, T. & Erra-Balsells, R. (2015) . Easy protocol for making a good E-sinapinic acid matrix for neutral and sulfated carbohydrate MALDI-MS analysis. Analytical Methods, 7(19), 8478-8483.
http://dx.doi.org/10.1039/c5ay01484k
---------- CHICAGO ----------
Salum, M.L., Schmidt De León, T., Erra-Balsells, R. "Easy protocol for making a good E-sinapinic acid matrix for neutral and sulfated carbohydrate MALDI-MS analysis" . Analytical Methods 7, no. 19 (2015) : 8478-8483.
http://dx.doi.org/10.1039/c5ay01484k
---------- MLA ----------
Salum, M.L., Schmidt De León, T., Erra-Balsells, R. "Easy protocol for making a good E-sinapinic acid matrix for neutral and sulfated carbohydrate MALDI-MS analysis" . Analytical Methods, vol. 7, no. 19, 2015, pp. 8478-8483.
http://dx.doi.org/10.1039/c5ay01484k
---------- VANCOUVER ----------
Salum, M.L., Schmidt De León, T., Erra-Balsells, R. Easy protocol for making a good E-sinapinic acid matrix for neutral and sulfated carbohydrate MALDI-MS analysis. Anal. Methods. 2015;7(19):8478-8483.
http://dx.doi.org/10.1039/c5ay01484k