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Abstract:

Objectives: The use of induced pluripotent stem (iPS) cells as an alternative to embryonic stem cells to produce transgenic animals requires the development of a biotechnological platform for their generation. In this study, different strategies for the generation of bovine and porcine iPS cells were evaluated. Lentiviral vectors were used to deliver human factors OCT4, SOX2, KLF4 and c-MYC (OKSM) into bovine and porcine embryonic fibroblasts and different culture conditions were evaluated. Results: Protocols based on the integrative lentiviral vector STEMCCA produced porcine iPS-like cells more efficiently than in bovine cells. The iPS-like cells generated displayed stem cell features; however, expression of exogenous factors was maintained along at least 12 passages. Since inactivation of the exogenous factors is still a major bottleneck for establishing fully reprogrammed iPS cells, defining culture conditions that support endogenous OKSM expression is critical for the efficient generation of farm animals' iPS cells. © 2018 The Author(s).

Registro:

Documento: Artículo
Título:Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system
Autor:Canizo, J.R.; Vazquez Echegaray, C.; Klisch, D.; Aller, J.F.; Paz, D.A.; Alberio, R.H.; Alberio, R.; Guberman, A.S.
Filiación:Departamento de Producción Animal, INTA EEA Balcarce, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
CONICET, Universidad de Buenos Aires, Instituto de Química Biológica (IQUIBICEN), Buenos Aires, Argentina
School of Biosciences, University of Nottingham, Nottingham, LE12 5RD, United Kingdom
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Fisiología Biología Molecular y Neurociencias (IFIBYNE), Universidad de Buenos Aires, CONICET, Buenos Aires, Argentina
Facultad de Ciencias Agrarias, Universidad Nacional de Mar Del Plata, Mar del Plata, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bovine and porcine fibroblasts; iPS-like cells; Lentiviral vectors; Reprogramming; STEMCCA; kruppel like factor; octamer transcription factor 4; transcription factor Sox; animal; bovine; cell differentiation; fibroblast; gene expression regulation; human; induced pluripotent stem cell; Lentivirus; metabolism; nuclear reprogramming; physiology; pig; Animals; Cattle; Cell Differentiation; Cellular Reprogramming; Fibroblasts; Gene Expression Regulation; Humans; Induced Pluripotent Stem Cells; Kruppel-Like Transcription Factors; Lentivirus; Octamer Transcription Factor-3; SOXB1 Transcription Factors; Swine
Año:2018
Volumen:11
Número:1
DOI: http://dx.doi.org/10.1186/s13104-018-3627-8
Título revista:BMC Research Notes
Título revista abreviado:BMC Res. Notes
ISSN:17560500
CAS:Kruppel-Like Transcription Factors; Octamer Transcription Factor-3; SOXB1 Transcription Factors
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17560500_v11_n1_p_Canizo

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Citas:

---------- APA ----------
Canizo, J.R., Vazquez Echegaray, C., Klisch, D., Aller, J.F., Paz, D.A., Alberio, R.H., Alberio, R.,..., Guberman, A.S. (2018) . Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system. BMC Research Notes, 11(1).
http://dx.doi.org/10.1186/s13104-018-3627-8
---------- CHICAGO ----------
Canizo, J.R., Vazquez Echegaray, C., Klisch, D., Aller, J.F., Paz, D.A., Alberio, R.H., et al. "Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system" . BMC Research Notes 11, no. 1 (2018).
http://dx.doi.org/10.1186/s13104-018-3627-8
---------- MLA ----------
Canizo, J.R., Vazquez Echegaray, C., Klisch, D., Aller, J.F., Paz, D.A., Alberio, R.H., et al. "Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system" . BMC Research Notes, vol. 11, no. 1, 2018.
http://dx.doi.org/10.1186/s13104-018-3627-8
---------- VANCOUVER ----------
Canizo, J.R., Vazquez Echegaray, C., Klisch, D., Aller, J.F., Paz, D.A., Alberio, R.H., et al. Exogenous human OKSM factors maintain pluripotency gene expression of bovine and porcine iPS-like cells obtained with STEMCCA delivery system. BMC Res. Notes. 2018;11(1).
http://dx.doi.org/10.1186/s13104-018-3627-8