Artículo
Abstract:
DNAzymes and Ribozymes find applications as inhibitors of gene expression and in detection systems such as biosensors, among others. In particular, we are interested in the properties of hammerhead ribozymes carrying 2'-C-methylnucleotides and 10-23 DNAzymes containing (2'R) or (2'S)- 2'-C-methyl 2'-deoxynucleotides. In this work a new synthesis of 2'-C-Methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2'-hydroxyl group. Comparison to previous protecting strategies as well as the stability under oligonucleotide synthesis conditions is discussed.2'-C-methyl-2'-deoxynucleosides show differential preferred conformations depending on the configuration of the 2'-carbon. The influence of these modifications on the activity of 10-23 DNAzymes is also assessed.
Registro:
| Documento: | Artículo |
| Título: | DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. |
| Autor: | Pontiggia, R.; Robaldo, L.; Montserrat, J.; Iribarren, A. |
| Filiación: | INGEBI, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina. |
| Palabras clave: | 2' C methyluridine; 2'-C-methyluridine; deoxyribonucleotide; deoxyribozyme; drug derivative; hammerhead ribozyme; organophosphorus compound; phosphoramidous acid; ribonucleotide; ribozyme; uridine; deoxyribonucleotide; deoxyribozyme; article; chemistry; synthesis; Deoxyribonucleotides; DNA, Catalytic; Organophosphorus Compounds; Ribonucleotides; RNA, Catalytic; Uridine; Deoxyribonucleotides; DNA, Catalytic; Organophosphorus Compounds; Ribonucleotides; RNA, Catalytic; Uridine |
| Año: | 2008 |
| Número: | 52 |
| Página de inicio: | 521 |
| Página de fin: | 522 |
| Título revista: | Nucleic acids symposium series (2004) |
| Título revista abreviado: | Nucleic Acids Symp Ser (Oxf) |
| ISSN: | 17468272 |
| CAS: | deoxyribozyme, 206668-15-7; uridine, 58-96-8; 2'-C-methyluridine; DNA, Catalytic; Deoxyribonucleotides; Organophosphorus Compounds; RNA, Catalytic; Ribonucleotides; Uridine, 58-96-8; hammerhead ribozyme; phosphoramidite; 2'-C-methyluridine; Deoxyribonucleotides; DNA, Catalytic; hammerhead ribozyme; Organophosphorus Compounds; phosphoramidite; Ribonucleotides; RNA, Catalytic; Uridine |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia |
Citas:
---------- APA ----------
Pontiggia, R., Robaldo, L., Montserrat, J. & Iribarren, A. (2008) . DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. Nucleic acids symposium series (2004)(52), 521-522.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia [ ]
---------- CHICAGO ----------
Pontiggia, R., Robaldo, L., Montserrat, J., Iribarren, A. "DNAzymes and ribozymes carrying 2'-C-methyl nucleotides." Nucleic acids symposium series (2004), no. 52 (2008) : 521-522.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia [ ]
---------- MLA ----------
Pontiggia, R., Robaldo, L., Montserrat, J., Iribarren, A. "DNAzymes and ribozymes carrying 2'-C-methyl nucleotides." Nucleic acids symposium series (2004), no. 52, 2008, pp. 521-522.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia [ ]
---------- VANCOUVER ----------
Pontiggia, R., Robaldo, L., Montserrat, J., Iribarren, A. DNAzymes and ribozymes carrying 2'-C-methyl nucleotides. Nucleic Acids Symp Ser (Oxf). 2008(52):521-522.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17468272_v_n52_p521_Pontiggia [ ]
