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Vitiello, G.; Fragneto, G.; Petruk, A.A.; Falanga, A.; Galdiero, S.; D'Ursi, A.M.; Merlino, A.; D'Errico, G. "Cholesterol modulates the fusogenic activity of a membranotropic domain of the FIV glycoprotein gp36" (2013) Soft Matter. 9(28):6442-6456
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Abstract:

Lipid composition of viral envelopes is usually rich in sphingolipids and cholesterol (CHOL). These components have a stiffening effect on the membrane, thus enhancing the energetic barrier to be overcome for its fusion with the T-cell plasma membrane, a fundamental step of the infection process. In this work, we demonstrate that the octapeptide (C8) corresponding to the Trp 770-Ile777 sequence of the Feline Immunodeficiency Virus gp36 is highly effective in inducing the fusion of palmitoyl oleoyl phosphatidylcholine (POPC)/sphingomyelin (SM)/CHOL membranes. We analyze the molecular mechanism of the C8-membrane interactions combining Neutron Reflectivity (NR) and Electron Spin Resonance (ESR) experiments, and molecular dynamics simulations. A strict interplay among the different lipids in the peptide-induced fusion mechanism is highlighted. Since CHOL preferentially locates close to SM, POPC molecules remain relatively free to interact with the peptide, driving its positioning at the membrane interface. Here, C8 comes in contact with CHOL-interacting SM molecules, causing a strong perturbation of acyl chain ordering, which is a necessary condition for membrane fusion. Our findings suggest that CHOL rules, by an indirect mechanism, the activity of viral fusion glycoproteins. © The Royal Society of Chemistry 2013.

Registro:

Documento: Artículo
Título:Cholesterol modulates the fusogenic activity of a membranotropic domain of the FIV glycoprotein gp36
Autor:Vitiello, G.; Fragneto, G.; Petruk, A.A.; Falanga, A.; Galdiero, S.; D'Ursi, A.M.; Merlino, A.; D'Errico, G.
Filiación:Department of Chemical Sciences, University of Naples Federico II, Naples, Italy
CSGI (Consorzio per Lo Sviluppo Dei Sistemi A Grande Interfase), Florence, Italy
Institut Laue-Langevin, Grenoble, France
Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE-CONICET, University of Buenos Aires, Buenos Aires, Argentina
Department of Pharmacy, Centro Interuniversitario di Ricerca sui Peptidi Bioattivi, University of Naples Federico II, Naples, Italy
Department of Pharmaceutical Science, University of Salerno, Fisciano, Italy
Istituto di Biostrutture e Bioimmagini, CNR, Naples, Italy
Palabras clave:Cell membranes; Glycoproteins; Molecular dynamics; Molecules; Peptides; Phospholipids; T-cells; Viruses; Energetic barriers; Immunodeficiency virus; Membrane interactions; Molecular dynamics simulations; Molecular mechanism; Neutron reflectivity; Phosphatidylcholine; Strong perturbations; Cholesterol
Año:2013
Volumen:9
Número:28
Página de inicio:6442
Página de fin:6456
DOI: http://dx.doi.org/10.1039/c3sm50553g
Título revista:Soft Matter
Título revista abreviado:Soft Matter
ISSN:1744683X
CODEN:SMOAB
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1744683X_v9_n28_p6442_Vitiello

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Citas:

---------- APA ----------
Vitiello, G., Fragneto, G., Petruk, A.A., Falanga, A., Galdiero, S., D'Ursi, A.M., Merlino, A.,..., D'Errico, G. (2013) . Cholesterol modulates the fusogenic activity of a membranotropic domain of the FIV glycoprotein gp36. Soft Matter, 9(28), 6442-6456.
http://dx.doi.org/10.1039/c3sm50553g
---------- CHICAGO ----------
Vitiello, G., Fragneto, G., Petruk, A.A., Falanga, A., Galdiero, S., D'Ursi, A.M., et al. "Cholesterol modulates the fusogenic activity of a membranotropic domain of the FIV glycoprotein gp36" . Soft Matter 9, no. 28 (2013) : 6442-6456.
http://dx.doi.org/10.1039/c3sm50553g
---------- MLA ----------
Vitiello, G., Fragneto, G., Petruk, A.A., Falanga, A., Galdiero, S., D'Ursi, A.M., et al. "Cholesterol modulates the fusogenic activity of a membranotropic domain of the FIV glycoprotein gp36" . Soft Matter, vol. 9, no. 28, 2013, pp. 6442-6456.
http://dx.doi.org/10.1039/c3sm50553g
---------- VANCOUVER ----------
Vitiello, G., Fragneto, G., Petruk, A.A., Falanga, A., Galdiero, S., D'Ursi, A.M., et al. Cholesterol modulates the fusogenic activity of a membranotropic domain of the FIV glycoprotein gp36. Soft Matter. 2013;9(28):6442-6456.
http://dx.doi.org/10.1039/c3sm50553g