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Abstract:

Chlorpyrifos (CPF) is a widely used organophosphate insecticide. The effect of chlorpyrifos exposure on acetylcholinesterase activity (AChE, biomarker of neurotoxicity) was evaluated in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). The animals were exposed to sublethal concentrations (1 and 5 μg CPF × L-1) or remained in control water for 96 h. AChE activity was inhibited 48% and 69% in C. decemmaculatus and 27% and 36% in G. affinis for 1 and 5 μg CPF × L-1, respectively. In vitro assays showed no differential sensitivity of the enzyme to CPF between species. However, the IC50 for in vitro exposure to chlorpyrifos-oxon (CPF-oxon, a product of the natural oxidative desulfuration of CPF) were 10.2 nM and 54.2 nM for C. decemmaculatus and G. affinis, respectively. Therefore, a different sensitivity of the enzyme between both species could explain the dissimilar behaviour of AChE after in vivo exposure to CPF. Copyright © 2017 Inderscience Enterprises Ltd.

Registro:

Documento: Artículo
Título:Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)
Autor:Bernal-Rey, D.L.; Dos Santos Afonso, M.; Menendez-Helman, R.J.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Inorgánica, Analítica y Química Física, Ciudad Universitaria Pabellón II 3er Piso, (C1428EHA), Buenos Aires, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Química Física de Los Materiales, Medio Ambiente y Energía (INQUIMAE), Ciudad Universitaria Pabellón II 3er Piso, (C1428EHA), Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Ciudad Universitaria Pabellón II 4to Piso, (C1428EHA), Buenos Aires, Argentina
CONICET-Universidad de Buenos Aires, Instituto de Química Biológica, Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Ciudad Universitaria Pabellón II 4to Piso, (C1428EHA), Buenos Aires, Argentina
Palabras clave:Acetylcholinesterase; AChE; Biomarkers; Chlorpyrifos; Cnesterodon decemmaculatus; Freshwater fish; Gambusia affinis; Pesticides; Teleost.
Año:2017
Volumen:8
Número:4
Página de inicio:316
Página de fin:330
DOI: http://dx.doi.org/10.1504/IJENVH.2017.088113
Título revista:International Journal of Environment and Health
Título revista abreviado:Int. J. Environ. Health
ISSN:17434955
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17434955_v8_n4_p316_BernalRey

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Citas:

---------- APA ----------
Bernal-Rey, D.L., Dos Santos Afonso, M. & Menendez-Helman, R.J. (2017) . Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). International Journal of Environment and Health, 8(4), 316-330.
http://dx.doi.org/10.1504/IJENVH.2017.088113
---------- CHICAGO ----------
Bernal-Rey, D.L., Dos Santos Afonso, M., Menendez-Helman, R.J. "Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)" . International Journal of Environment and Health 8, no. 4 (2017) : 316-330.
http://dx.doi.org/10.1504/IJENVH.2017.088113
---------- MLA ----------
Bernal-Rey, D.L., Dos Santos Afonso, M., Menendez-Helman, R.J. "Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis)" . International Journal of Environment and Health, vol. 8, no. 4, 2017, pp. 316-330.
http://dx.doi.org/10.1504/IJENVH.2017.088113
---------- VANCOUVER ----------
Bernal-Rey, D.L., Dos Santos Afonso, M., Menendez-Helman, R.J. Effects of chlorpyrifos on acetylcholinesterase activity in two freshwater fish species (Cnesterodon decemmaculatus and Gambusia affinis). Int. J. Environ. Health. 2017;8(4):316-330.
http://dx.doi.org/10.1504/IJENVH.2017.088113