Artículo

Artículo de Acceso Abierto. Puede ser descargado en su versión final
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The flavonoids 5,6,7,8,9-hydroxy chalcone, 3,7-hydroxy-4′methoxy flavone, 5,6,7,8-hydroxy-4′-methoxy flavone and 3,5,6,7,4′-hydroxy flavone can be detected only in non-mycorrhizal roots of white clover, but not in mycorrhizal roots, whereas the flavonoids acacetin, quercetin and rhamnetin are only present in mycorrhizal roots. We tested the effect of several concentrations of these compounds on spore germination, hyphal growth, hyphal branching, formation of clusters of auxiliary cells and of secondary spores of the arbuscular mycorrhizal fungi Gigaspora rosea, Gigaspora margarita, Glomus mosseae and Glomus intraradices. Our results indicate that depending on the flavonoid, the tested compounds are involved at different stages in the regulation of mycorrhization. This hypothesis is strengthened by their differing effect on several AM fungal growth parameters. Furthermore, our study provides more data on the AM fungus genus/species specificity of flavonoids. © 2005 Taylor & Francis.

Registro:

Documento: Artículo
Título:Flavonoids exclusively present in mycorrhizal roots of white clover exhibit a different effect on arbuscular mycorrhizal fungi than flavonoids exclusively present in non-mycorrhizal roots of white clover
Autor:Scervino, J.M.; Ponce, M.A.; Erra-Bassells, R.; Vierheilig, H.; Ocampo, J.A.; Godeas, A.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Institut für Pflanzenschutz, Universität für Bodenkultur Wien, Wien, Austria
Departamento de Microbiología, Estación Experimental de Zaidín, CSIC, Granada, Spain
Departamento de Microbiología del Suelo, Estación Experimental del Zaidín, CSIC, Prof. Albareda 1, E-18008 Granada, Spain
Palabras clave:Arbuscular mycorrhiza; Flavonoid; Glomales; Regulation; Signal molecule; Symbiosis; Arbuscular; Bacteria (microorganisms); Fungi; Gigaspora margarita; Gigaspora rosea; Glomerales; Glomus intraradices; Glomus mosseae; Trifolium repens
Año:2005
Volumen:1
Número:1
Página de inicio:15
Página de fin:22
DOI: http://dx.doi.org/10.1080/17429140500192597
Handle:http://hdl.handle.net/20.500.12110/paper_17429145_v1_n1_p15_Scervino
Título revista:Journal of Plant Interactions
Título revista abreviado:J. Plant Interact.
ISSN:17429145
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_17429145_v1_n1_p15_Scervino.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17429145_v1_n1_p15_Scervino

Referencias:

  • Akiyama, K., Matsuoka, H., Hayashi, H., Isolation and identification of a phosphate deficiency-induced C-glycosylflavonoid that stimulates arbuscular mycorrhiza formation in melon roots (2002) Mol Plant Microbe Interaction, 15, pp. 334-340
  • Baptista, M.J., Siqueira, J.O., Efeito de flavonóides na germinação e no crescimento assimbiótico de fungos micorrízicos vesículo-arbusculares (1994) Rev Brasil Fisiolog Vegetal, 6, pp. 127-134
  • Bécard, G., Douds, D.D., Pfeffer, P.E., Extensive in vitro hyphal growth of vesicular-arbuscular mycorrhizal fungi in the presence of CO2 and flavonols (1992) Appl Environment Microbiol, 58, pp. 821-825
  • Bel-Rhlid, R., Chabot, S., Piché, Y., Chenevert, R., Isolation and identification of flavonoids from Ri T-DNA-transformed roots (Daucus carota) and their significance in vesicular-arbuscular mycorrhiza (1993) Phytochemistry, 33, pp. 1369-1371
  • Bentivenga, S.P., Morton, J.B., A monograph of the genus Gigaspora, incorporating developmental patterns of morphological characters (1995) Mycologia, 87, pp. 720-732
  • Catford, J.G., Staehelin, C., Lerat, S., Piché, Y., Vierheilig, H., Suppression of arbuscular mycorrhizal colonization and nodulation in split-root systems of alfalfa after pre-inoculation and treatment with Nod factors (2003) J Exp Botany, 54, pp. 1481-1487
  • Chabot, S., Bel-Rhlid, T., Chenevert, R., Piche, Y., Hyphae growth promotion in vitro of the VA mycorrhizal fungus, Gigaspora margarita Becker & Hall, by the activity of structurally specific flavonoid compounds under CO2-enriched conditions (1992) New Phytologist, 122, pp. 461-467
  • Fawe, A., Abou-Zaid, M., Menzies, J., Belanger, R.R., Silicon-mediated accumulation of flavonoid phytoalexins in cucumber (1998) Phytopathology, 88, pp. 396-401
  • Fracchia, S., (2002) Hongos Saprotrofos del Suelo Como Microorganismos Auxiliares de la Micorrización, , PhD Thesis, Universidad de Buenos Aires, Argentina
  • Garcia-Garrido, J.M., Ocampo, J.A., Regulation of the plant defence response in arbuscular mycorrhizal symbiosis (2002) J Exp Botany, 53, pp. 1377-1386
  • Gerdemann, J.W., Relation of a large soil-borne spore to phycomycetous mycorrhizal infections (1955) Mycologia, 47, pp. 619-632
  • Gianinazzi-Pearson, V., Branzanti, B., Gianinazzi, S., In vitro enhancement of spore germination and early hyphal growth of a vesicular-arbuscular mycorrhizal fungus by host root exudates and plant flavonoids (1989) Symbiosis, 7, pp. 243-255
  • Giovannetti, M., Mosse, B., An evaluation of techniques for measuring vesicular-arbuscular mycorrhizal infection in roots (1980) New Phytologist, 84, pp. 489-500
  • Giovannetti, M., Sbrana, C., Citernesi, A.S., Avio, L., Analysis of factor involved in fungal recognition responses to host-derived signals by arbuscular mycorrhizal fungi (1996) New Phytologist, 133, pp. 65-71
  • Harley, J.L., Harley, E.L., A check-list of mycorrhiza in the British flora (1987) New Phytologist (Suppl.), 105, pp. 1-102
  • Hewitt, E.J., (1952) Sand Water Culture Methods Used in the Study of Plant Nutrition, p. 22. , Wallingford, UK: Commonwealth Agriculture Bureau, Technical communication
  • Juge, C., Samson, J., Bastien, C., Vierheilig, H., Coughlan, A., Piche, Y., Breaking dormancy in spores of the arbuscular mycorrhizal fungus Glomus intraradices: A critical cold-storage period (2002) Mycorrhiza, 12, pp. 37-42
  • Kape, R., Parniske, M., Brandt, S., Werner, D., Isoliquiritigenin, a strong nod gene- and glyceollin resistance-inducing flavonoid from soybean root exudate (1992) Appl Environment Microbiol, 58, pp. 1705-1710
  • Kape, R., Wex, K., Parniske, M., Görge, E., Wetzel, A., Werner, D., Legume root metabolites and VA-mycorrhiza development (1992) J Plant Physiologist, 141, pp. 54-60
  • Larose, G., Chenevert, R., Moutoglis, P., Gagne, S., Piche, Y., Vierheilig, H., Flavonoid levels in roots of Medicago sativa are modulated by the developmental stage of the symbiosis and the root colonizing arbuscular mycorrhizal fungus (2002) J Plant Physiol, 159, pp. 1329-1339
  • Marsh, B.A.B., Measurement of length in random arrangements of lines (1971) J Applied Ecol, 8, pp. 265-270
  • Mingorance, M.D., Focused microwave-assisted digestion of vegetal materials for the determination of essential mineral nutrients (2002) Ann Bioanalyt Chem, 373, pp. 153-158
  • Morandi, D., Occurrence of phytoalexins and phenolic compounds in endomycorrhizal interactions, and their potential role in biological control (1996) Plant Soil, 185, pp. 241-251
  • Morandi, D., Branzanti, B., Gianinazzi-Pearson, V., Effect of some plant flavonoid on in vitro behavior of an arbuscular mycorrhizal fungus (1992) Agronomie, 12, pp. 811-816
  • Mosse, B., The establishment of vesicular arbuscular mycorrhiza under aseptic conditions (1962) J Gen Microbiol, 27, pp. 509-520
  • Nair, M.G., Safir, G.R., Siqueira, J.O., Isolation and identification of vesicular-arbuscular mycorrhizal stimulatory compounds from clover (Trifolium repens) roots (1991) Appl Environment Microbiol, 57, pp. 434-439
  • Phillips, J.M., Hayman, D.S., Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection (1970) Trans Br Mycologic Soc, 55, pp. 158-161
  • Phillips, D.A., Tsai, S.M., Flavonoids as plant signals to the rhizosphere microbes (1992) Mycorrhiza, 1, pp. 55-58
  • Pinior, A., Wyss, U., Piche, Y., Vierheilig, H., Plants colonized by AM fungi regulate further root colonization by AM fungi through altered root exudation (1999) Can J Botany-Rev Can Botanique, 77, pp. 891-897
  • Poulin, M.J., Simard, J., Catford, J.G., Labrie, F., Piche, Y., Response of symbiotic endomycorrhizal fungi to estrogens and antiestrogens (1997) Mol Plant Microbe Interaction, 10, pp. 481-487
  • Ponce, M.A., Scervino, J.M., Erra Balsells, R., Ocampo, J.A., Godeas, A., Flavonoids from shoots and roots of Trifolium repens (white clover) grown with and without the arbuscular mycorrhizal fungus Glomus intraradices (2004) Phytochemistry, 65, pp. 1925-1930
  • Dyac, S., Salatino, M.L.F., Foliar flavonoids of Annonaceae from Brazil: Taxonomic significance (2000) Phytochemistry, 55, pp. 567-573
  • Santos, D.Y.A.C., Salatino, M.L.F., Salatino, A., Flavonoids of species of Cuphea (Lythraceae) from Brazil (1995) Biochem Systemat Ecol, 23, pp. 99-103
  • Serra-Bonvehi, J., Ventura-Coll, F., Escola-Jorda, R., The composition, active components and bacteriostatic activity of propolis in dietetics (1994) J Am Oil Chem, 71, pp. 529-532
  • Siqueira, J.O., Safir, G.R., Nair, M.G., Stimulation of vesicular-arbuscular mycorrhiza formation and growth of white clover by flavonoid compounds (1991) New Phytologist, 118, pp. 87-93
  • Tsai, S.M., Phillips, D.A., Flavonoids released naturally from alfalfa promote development of symbiotic Glomus spores in vitro (1991) Appl Environment Microbiol, 57, pp. 1485-1488
  • Vierheilig, H., Regulatory mechanisms during the plant-arbuscular mycorrhizal fungus interaction (2004) Can J Botany, 82, pp. 1166-1176
  • Vierheilig, H., Further root colonization by arbuscular mycorrhizal fungi in already mycorrhizal plants is suppressed after a critical level of root colonization (2004) J Plant Physiol, 161, pp. 339-341
  • Vierheilig, H., Garcia-Garrido, J.M., Wyss, U., Piché, Y., Systemic suppression of mycorrhizal colonization of barley roots already colonized by AM fungi (2000) Soil Biol Biochem, 32, pp. 589-595
  • Vierheilig, H., Maier, W., Wyss, U., Samson, J., Strack, D., Piché, Y., Cyclohexenone derivative- and phosphate-levels in split-root systems and their role in the systemic suppression of mycorrhization in precolonized barley plants (2000) J Plant Physiol, 157, pp. 593-599
  • Vierheilig, H., Bago, B., Albrecht, C., Poulin, M.J., Piche, Y., Flavonoids and arbuscular-mycorrhizal fungi (1998) Flavonoids in the Living System, pp. 9-33. , Manthey JA, Buslig BS, editors. New York: Plenum Press
  • Vierheilig, H., Piche, Y., (2002) Signalling in Arbuscular Mycorrhiza: Facts and Hypotheses, pp. 23-39. , Buslig B, Manthey J, editors. Flavonoids in cell function. New York: Kluwer Academic/Plenum Press
  • Winkel-Shirley, B., Biosynthesis of flavonoids and effects of stress (2002) Curr Opin Plant Biol, 5, pp. 218-223

Citas:

---------- APA ----------
Scervino, J.M., Ponce, M.A., Erra-Bassells, R., Vierheilig, H., Ocampo, J.A. & Godeas, A. (2005) . Flavonoids exclusively present in mycorrhizal roots of white clover exhibit a different effect on arbuscular mycorrhizal fungi than flavonoids exclusively present in non-mycorrhizal roots of white clover. Journal of Plant Interactions, 1(1), 15-22.
http://dx.doi.org/10.1080/17429140500192597
---------- CHICAGO ----------
Scervino, J.M., Ponce, M.A., Erra-Bassells, R., Vierheilig, H., Ocampo, J.A., Godeas, A. "Flavonoids exclusively present in mycorrhizal roots of white clover exhibit a different effect on arbuscular mycorrhizal fungi than flavonoids exclusively present in non-mycorrhizal roots of white clover" . Journal of Plant Interactions 1, no. 1 (2005) : 15-22.
http://dx.doi.org/10.1080/17429140500192597
---------- MLA ----------
Scervino, J.M., Ponce, M.A., Erra-Bassells, R., Vierheilig, H., Ocampo, J.A., Godeas, A. "Flavonoids exclusively present in mycorrhizal roots of white clover exhibit a different effect on arbuscular mycorrhizal fungi than flavonoids exclusively present in non-mycorrhizal roots of white clover" . Journal of Plant Interactions, vol. 1, no. 1, 2005, pp. 15-22.
http://dx.doi.org/10.1080/17429140500192597
---------- VANCOUVER ----------
Scervino, J.M., Ponce, M.A., Erra-Bassells, R., Vierheilig, H., Ocampo, J.A., Godeas, A. Flavonoids exclusively present in mycorrhizal roots of white clover exhibit a different effect on arbuscular mycorrhizal fungi than flavonoids exclusively present in non-mycorrhizal roots of white clover. J. Plant Interact. 2005;1(1):15-22.
http://dx.doi.org/10.1080/17429140500192597