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Abstract:

The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.

Registro:

Documento: Conferencia
Título:Analysis of persistence during intracellular actin-based transport mediated by molecular motors
Autor:Pallavicini, C.; Despósito, M.A.; Levi, V.; Bruno, L.
Filiación:Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Palabras clave:Cytology; Anomalous diffusion; Cell cytoplasm; Function of time; Molecular motors; Spatio-temporal resolution; Tracking techniques; Turning angles; Xenopus laevis; Proteins
Año:2010
Volumen:246
DOI: http://dx.doi.org/10.1088/1742-6596/246/1/012038
Handle:http://hdl.handle.net/20.500.12110/paper_17426588_v246_n_p_Pallavicini
Título revista:Journal of Physics: Conference Series
Título revista abreviado:J. Phys. Conf. Ser.
ISSN:17426588
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_17426588_v246_n_p_Pallavicini.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17426588_v246_n_p_Pallavicini

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Citas:

---------- APA ----------
Pallavicini, C., Despósito, M.A., Levi, V. & Bruno, L. (2010) . Analysis of persistence during intracellular actin-based transport mediated by molecular motors. Journal of Physics: Conference Series, 246.
http://dx.doi.org/10.1088/1742-6596/246/1/012038
---------- CHICAGO ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L. "Analysis of persistence during intracellular actin-based transport mediated by molecular motors" . Journal of Physics: Conference Series 246 (2010).
http://dx.doi.org/10.1088/1742-6596/246/1/012038
---------- MLA ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L. "Analysis of persistence during intracellular actin-based transport mediated by molecular motors" . Journal of Physics: Conference Series, vol. 246, 2010.
http://dx.doi.org/10.1088/1742-6596/246/1/012038
---------- VANCOUVER ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L. Analysis of persistence during intracellular actin-based transport mediated by molecular motors. J. Phys. Conf. Ser. 2010;246.
http://dx.doi.org/10.1088/1742-6596/246/1/012038