Abstract:
The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.
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Documento: |
Conferencia
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Título: | Analysis of persistence during intracellular actin-based transport mediated by molecular motors |
Autor: | Pallavicini, C.; Despósito, M.A.; Levi, V.; Bruno, L. |
Filiación: | Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
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Palabras clave: | Cytology; Anomalous diffusion; Cell cytoplasm; Function of time; Molecular motors; Spatio-temporal resolution; Tracking techniques; Turning angles; Xenopus laevis; Proteins |
Año: | 2010
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Volumen: | 246
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DOI: |
http://dx.doi.org/10.1088/1742-6596/246/1/012038 |
Título revista: | Journal of Physics: Conference Series
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Título revista abreviado: | J. Phys. Conf. Ser.
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ISSN: | 17426588
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_17426588_v246_n_p_Pallavicini.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17426588_v246_n_p_Pallavicini |
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Citas:
---------- APA ----------
Pallavicini, C., Despósito, M.A., Levi, V. & Bruno, L.
(2010)
. Analysis of persistence during intracellular actin-based transport mediated by molecular motors. Journal of Physics: Conference Series, 246.
http://dx.doi.org/10.1088/1742-6596/246/1/012038---------- CHICAGO ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L.
"Analysis of persistence during intracellular actin-based transport mediated by molecular motors"
. Journal of Physics: Conference Series 246
(2010).
http://dx.doi.org/10.1088/1742-6596/246/1/012038---------- MLA ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L.
"Analysis of persistence during intracellular actin-based transport mediated by molecular motors"
. Journal of Physics: Conference Series, vol. 246, 2010.
http://dx.doi.org/10.1088/1742-6596/246/1/012038---------- VANCOUVER ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L. Analysis of persistence during intracellular actin-based transport mediated by molecular motors. J. Phys. Conf. Ser. 2010;246.
http://dx.doi.org/10.1088/1742-6596/246/1/012038