Conferencia
Abstract:
The displacement of particles or probes in the cell cytoplasm as a function of time is characterized by different anomalous diffusion regimes. The transport of large cargoes, such as organelles, vesicles or large proteins, involves the action of ATP-consuming molecular motors. We investigate the motion of pigment organelles driven by myosin-V motors in Xenopus laevis melanocytes using a high spatio-temporal resolution tracking technique. By analyzing the turning angles (φ) of the obtained 2D trajectories as a function of the time lag, we determine the critical time of the transition between anticorrelated and directed motion as the time when the turning angles begin to concentrate around φ 0. We relate this transition with the crossover from subdiffusive to superdiffusive behavior observed in a previous work [5]. We also assayed the properties of the trajectories in cells with inhibited myosin activity, and we can compare the results in the presence and absence of active motors. © 2010 IOP Publishing Ltd.
Registro:
| Documento: | Conferencia |
| Título: | Analysis of persistence during intracellular actin-based transport mediated by molecular motors |
| Autor: | Pallavicini, C.; Despósito, M.A.; Levi, V.; Bruno, L. |
| Filiación: | Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina |
| Palabras clave: | Cytology; Anomalous diffusion; Cell cytoplasm; Function of time; Molecular motors; Spatio-temporal resolution; Tracking techniques; Turning angles; Xenopus laevis; Proteins |
| Año: | 2010 |
| Volumen: | 246 |
| DOI: | http://dx.doi.org/10.1088/1742-6596/246/1/012038 |
| Título revista: | Journal of Physics: Conference Series |
| Título revista abreviado: | J. Phys. Conf. Ser. |
| ISSN: | 17426588 |
| PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_17426588_v246_n_p_Pallavicini.pdf |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17426588_v246_n_p_Pallavicini |
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Citas:
---------- APA ----------
Pallavicini, C., Despósito, M.A., Levi, V. & Bruno, L. (2010) . Analysis of persistence during intracellular actin-based transport mediated by molecular motors. Journal of Physics: Conference Series, 246.http://dx.doi.org/10.1088/1742-6596/246/1/012038
---------- CHICAGO ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L. "Analysis of persistence during intracellular actin-based transport mediated by molecular motors" . Journal of Physics: Conference Series 246 (2010).http://dx.doi.org/10.1088/1742-6596/246/1/012038
---------- MLA ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L. "Analysis of persistence during intracellular actin-based transport mediated by molecular motors" . Journal of Physics: Conference Series, vol. 246, 2010.http://dx.doi.org/10.1088/1742-6596/246/1/012038
---------- VANCOUVER ----------
Pallavicini, C., Despósito, M.A., Levi, V., Bruno, L. Analysis of persistence during intracellular actin-based transport mediated by molecular motors. J. Phys. Conf. Ser. 2010;246.http://dx.doi.org/10.1088/1742-6596/246/1/012038
