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Pesce, A.; Bustamante, J.P.; Bidon-Chanal, A.; Boechi, L.; Estrin, D.A.; Luque, F.J.; Sebilo, A.; Guertin, M.; Bolognesi, M.; Ascenzi, P.; Nardini, M. "The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity" (2016) FEBS Journal. 283(2):305-322
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Abstract:

A unique defense mechanisms by which Mycobacterium tuberculosis protects itself from nitrosative stress is based on the O2-dependent NO-dioxygenase (NOD) activity of truncated hemoglobin 2/2HbN (Mt2/2HbN). The NOD activity largely depends on the efficiency of ligand migration to the heme cavity through a two-tunnel (long and short) system; recently, it was also correlated with the presence at the Mt2/2HbN N-terminus of a short pre-A region, not conserved in most 2/2HbNs, whose deletion results in a drastic reduction of NO scavenging. In the present study, we report the crystal structure of Mt2/2HbN-ΔpreA, lacking the pre-A region, at a resolution of 1.53 Å. We show that removal of the pre-A region results in long range effects on the protein C-terminus, promoting the assembly of a stable dimer, both in the crystals and in solution. In the Mt2/2HbN-ΔpreA dimer, access of heme ligands to the short tunnel is hindered. Molecular dynamics simulations show that the long tunnel branch is the only accessible pathway for O2-ligand migration to/from the heme, and that the gating residue Phe(62)E15 partly restricts the diameter of the tunnel. Accordingly, kinetic measurements indicate that the kon value for peroxynitrite isomerization by Mt2/2HbN-ΔpreA-Fe(III) is four-fold lower relative to the full-length protein, and that NO scavenging by Mt2/2HbN-ΔpreA-Fe(II)-O2 is reduced by 35-fold. Therefore, we speculate that Mt2/2HbN evolved to host the pre-A region as a mechanism for preventing dimerization, thus reinforcing the survival of the microorganism against the reactive nitrosative stress in macrophages. Database Coordinates and structure factors have been deposited in the Protein Data Bank under accession number 5AB8. Removal of the pre-A region in M. tuberculosis 2/2HbN (Mt2/2HbN-ΔpreA) results in dimerization and in a reduced access to the heme pocket. Kinetic measurements indicate a 4-fold decrease in kon for peroxynitrite isomerization and a 35-fold decrease in NO-scavenging relative to full-length Mt2/2HbN. Thus, the pre-A region might be involved in reinforcing survival of the microorganism against nitrosative stress in macrophages. © 2015 FEBS.

Registro:

Documento: Artículo
Título:The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity
Autor:Pesce, A.; Bustamante, J.P.; Bidon-Chanal, A.; Boechi, L.; Estrin, D.A.; Luque, F.J.; Sebilo, A.; Guertin, M.; Bolognesi, M.; Ascenzi, P.; Nardini, M.
Filiación:Department of Physics, University of Genova, Italy
Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, University of Buenos Aires, Argentina
Departament de Fisicoquímica, Institut de Biomedicina (IBUB), Facultat de Farmàcia, University of Barcelona, Santa Coloma de Gramenet, Spain
Department of Biochemistry, Microbiology and Bioinformatics, Laval UniversityQC, Canada
Department of Biosciences, University of Milano, Milano, I-20133, Italy
CNR-IBF, CIMAINA, University of Milan, Italy
Interdepartmental Laboratory of Electron Microscopy, Roma Tre University, Rome, Italy
National Institute of Biostructures and Biosystems, Rome, Italy
Palabras clave:2/2 hemoglobins; globin dynamics; heme/ligand tunneling; NO dioxygenase; truncated hemoglobins; bacterial protein; dioxygenase; heme; nitric oxide; peroxynitrous acid; truncated hemoglobin; chemistry; genetics; kinetics; metabolism; molecular dynamics; mutation; Mycobacterium tuberculosis; protein conformation; protein multimerization; X ray crystallography; Bacterial Proteins; Crystallography, X-Ray; Dioxygenases; Heme; Kinetics; Molecular Dynamics Simulation; Mutation; Mycobacterium tuberculosis; Nitric Oxide; Peroxynitrous Acid; Protein Conformation; Protein Multimerization; Truncated Hemoglobins
Año:2016
Volumen:283
Número:2
Página de inicio:305
Página de fin:322
DOI: http://dx.doi.org/10.1111/febs.13571
Título revista:FEBS Journal
Título revista abreviado:FEBS J.
ISSN:1742464X
CODEN:FJEOA
CAS:dioxygenase, 37292-90-3; heme, 14875-96-8; nitric oxide, 10102-43-9; peroxynitrous acid, 14691-52-2; Bacterial Proteins; Dioxygenases; Heme; Nitric Oxide; Peroxynitrous Acid; Truncated Hemoglobins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1742464X_v283_n2_p305_Pesce

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Citas:

---------- APA ----------
Pesce, A., Bustamante, J.P., Bidon-Chanal, A., Boechi, L., Estrin, D.A., Luque, F.J., Sebilo, A.,..., Nardini, M. (2016) . The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity. FEBS Journal, 283(2), 305-322.
http://dx.doi.org/10.1111/febs.13571
---------- CHICAGO ----------
Pesce, A., Bustamante, J.P., Bidon-Chanal, A., Boechi, L., Estrin, D.A., Luque, F.J., et al. "The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity" . FEBS Journal 283, no. 2 (2016) : 305-322.
http://dx.doi.org/10.1111/febs.13571
---------- MLA ----------
Pesce, A., Bustamante, J.P., Bidon-Chanal, A., Boechi, L., Estrin, D.A., Luque, F.J., et al. "The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity" . FEBS Journal, vol. 283, no. 2, 2016, pp. 305-322.
http://dx.doi.org/10.1111/febs.13571
---------- VANCOUVER ----------
Pesce, A., Bustamante, J.P., Bidon-Chanal, A., Boechi, L., Estrin, D.A., Luque, F.J., et al. The N-terminal pre-A region of Mycobacterium tuberculosis 2/2HbN promotes NO-dioxygenase activity. FEBS J. 2016;283(2):305-322.
http://dx.doi.org/10.1111/febs.13571