Artículo

Giordano, D.; Boechi, L.; Vergara, A.; Martí, M.A.; Samuni, U.; Dantsker, D.; Grassi, L.; Estrin, D.A.; Friedman, J.M.; Mazzarella, L.; Di Prisco, G.; Verde, C. "The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics" (2009) FEBS Journal. 276(8):2266-2277
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Abstract:

The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history. Non-Antarctic notothenioids belonging to the most basal families are a crucial source for understanding the evolution of hemoglobin in high-Antarctic cold-adapted fish. This paper focuses on the structure, function and evolution of the oxygen-transport system of Cottoperca gobio, a sub-Antarctic notothenioid fish of the family Bovichtidae, probably derived from ancestral species that evolved in the Antarctic region and later migrated to lower latitudes. Unlike most high-Antarctic notothenioids, but similar to many other acanthomorph teleosts, C. gobio has two major hemoglobins having the β chain in common. The oxygen-binding equilibria and kinetics of the two hemoglobins have been measured. Hb1 and Hb2 show strong modulation of oxygen-binding equilibria and kinetics by heterotropic effectors, with marked Bohr and Root effects. In Hb1 and Hb2, oxygen affinity and subunit cooperativity are slightly higher than in most high-Antarctic notothenioid hemoglobins. Hb1 and Hb2 show similar rebinding rates, but also show significant dynamic differences that are likely to have functional consequences. Molecular dynamic simulations of C. gobio Hb1 were performed on the dimeric protein in order to obtain a better understanding of the molecular basis of structure/function relationships. © 2009 FEBS.

Registro:

Documento: Artículo
Título:The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics
Autor:Giordano, D.; Boechi, L.; Vergara, A.; Martí, M.A.; Samuni, U.; Dantsker, D.; Grassi, L.; Estrin, D.A.; Friedman, J.M.; Mazzarella, L.; Di Prisco, G.; Verde, C.
Filiación:Institute of Protein Biochemistry, CNR, Via Pietro Castellino 111, I-80131 Naples, Italy
Institute of Protein Biochemistry, National Research Council (CNR), Naples, Italy
Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, Argentina
Department of Chemistry, University of Naples 'Federico II', Naples, Italy
Department of Chemistry and Biochemistry, Queens College, City University New York, Flushing, NY, United States
Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY, United States
Palabras clave:Antarctica; Computer simulation; Hemoglobin; Ligand-binding properties; Oxygen affinity; hemoglobin; hemoglobin beta chain; protein; Antarctica; article; Bohr effect; Cottoperca gobio; fish; hemoglobin analysis; kinetics; latitude; migration; modulation; molecular dynamics; nonhuman; oxygen affinity; priority journal; simulation; teleost; Amino Acid Sequence; Animals; Binding Sites; Computer Simulation; Fishes; Hemoglobins; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Oxygen; Phylogeny; Species Specificity; Structure-Activity Relationship; Thermodynamics; Acanthomorpha; Bovichthyidae; Cottoperca gobio; Notothenioidei; Perciformes; Teleostei
Año:2009
Volumen:276
Número:8
Página de inicio:2266
Página de fin:2277
DOI: http://dx.doi.org/10.1111/j.1742-4658.2009.06954.x
Título revista:FEBS Journal
Título revista abreviado:FEBS J.
ISSN:1742464X
CAS:hemoglobin, 9008-02-0; protein, 67254-75-5; Hemoglobins; Oxygen, 7782-44-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1742464X_v276_n8_p2266_Giordano

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Citas:

---------- APA ----------
Giordano, D., Boechi, L., Vergara, A., Martí, M.A., Samuni, U., Dantsker, D., Grassi, L.,..., Verde, C. (2009) . The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics. FEBS Journal, 276(8), 2266-2277.
http://dx.doi.org/10.1111/j.1742-4658.2009.06954.x
---------- CHICAGO ----------
Giordano, D., Boechi, L., Vergara, A., Martí, M.A., Samuni, U., Dantsker, D., et al. "The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics" . FEBS Journal 276, no. 8 (2009) : 2266-2277.
http://dx.doi.org/10.1111/j.1742-4658.2009.06954.x
---------- MLA ----------
Giordano, D., Boechi, L., Vergara, A., Martí, M.A., Samuni, U., Dantsker, D., et al. "The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics" . FEBS Journal, vol. 276, no. 8, 2009, pp. 2266-2277.
http://dx.doi.org/10.1111/j.1742-4658.2009.06954.x
---------- VANCOUVER ----------
Giordano, D., Boechi, L., Vergara, A., Martí, M.A., Samuni, U., Dantsker, D., et al. The hemoglobins of the sub-Antarctic fish Cottoperca gobio, a phyletically basal species - Oxygen-binding equilibria, kinetics and molecular dynamics. FEBS J. 2009;276(8):2266-2277.
http://dx.doi.org/10.1111/j.1742-4658.2009.06954.x