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Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos. © 2016 Plachta et al.


Documento: Artículo
Título:Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos
Autor:Zhao, Z.W.; White, M.D.; Bissiere, S.; Levi, V.; Plachta, N.
Filiación:Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A STAR), 61 Biopolis Drive, Singapore, 138673, Singapore
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Conicet, C1428EHA, Argentina
Palabras clave:animal; cell nucleus; embryology; fluorescence microscopy; gene expression regulation; genetic epigenesis; genetics; mammal; single cell analysis; spatiotemporal analysis; transcription initiation; Animals; Cell Nucleus; Epigenesis, Genetic; Gene Expression Regulation; Mammals; Microscopy, Fluorescence; Single-Cell Analysis; Spatio-Temporal Analysis; Transcriptional Activation
Título revista:BMC Biology
Título revista abreviado:BMC Biol.


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---------- APA ----------
Zhao, Z.W., White, M.D., Bissiere, S., Levi, V. & Plachta, N. (2016) . Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos. BMC Biology, 14(1).
---------- CHICAGO ----------
Zhao, Z.W., White, M.D., Bissiere, S., Levi, V., Plachta, N. "Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos" . BMC Biology 14, no. 1 (2016).
---------- MLA ----------
Zhao, Z.W., White, M.D., Bissiere, S., Levi, V., Plachta, N. "Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos" . BMC Biology, vol. 14, no. 1, 2016.
---------- VANCOUVER ----------
Zhao, Z.W., White, M.D., Bissiere, S., Levi, V., Plachta, N. Quantitative imaging of mammalian transcriptional dynamics: From single cells to whole embryos. BMC Biol. 2016;14(1).