Artículo

Ronda, A.C.; Oliva, A.L.; Arias, A.H.; Orazi, M.M.; Marcovecchio, J.E."Biomarker Responses to Polycyclic Aromatic Hydrocarbons in the Native Fish Ramnogaster arcuata, South America" (2019) International Journal of Environmental Research. 13(1):77-89
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Abstract:

Quantification of polycyclic aromatic hydrocarbons (PAHs) in Bahía Blanca Estuary (BBE, Argentina) fish samples (Ramnogaster arcuata) was performed to evaluate the environmental impact through anthropogenic activity. In addition, several metabolic enzyme activities (Aspartate aminotransferase—AST, Alanine aminotransferase—ALT, Lactate dehydrogenase—LDH, Creatine kinase—CK and, Alkaline phosphatase—ALP), protein content and lipid peroxidation as oxidative stress biomarker were analyzed in muscle and liver and related to tisular PAHs levels. Results showed low to moderate PAHs levels in R. arcuata muscle (9.34–41.25 ng/g, wet weight) with a marked predominance of two/three ringed compounds (phenanthrene > naphthalene > acenaphthene > acenaphthylene > fluoranthene). Fluoranthene, pyrene, benzo-[b]fluoranthene and benzo-[a]pyrene concentrations correlated positively with hepatic AST and ALT and negatively with muscular proteins and hepatic lipid peroxidation. 2-metil-naphthalene and acenaphthene levels correlated negatively with LDH in muscle and positively with lipid peroxidation in liver tissue. Correlation of PAHs with metabolic enzymes, proteins and lipid peroxidation indicated a differential metabolization and suggesting that hepatic AST/ALT could be used as PAHs biotransformation biomarkers and muscular LDH as a stress oxidation biomarker in R. arcuata. In addition, CK activity was suggested as a good index of muscular health. The obtained results highlighted the significance of using a set of integrated biomarkers to assess PAHs toxicity in fish inhabiting their natural ambient and confirm that R. arcuata could be used as a good bioindicator for marine areas. © 2018, University of Tehran.

Registro:

Documento: Artículo
Título:Biomarker Responses to Polycyclic Aromatic Hydrocarbons in the Native Fish Ramnogaster arcuata, South America
Autor:Ronda, A.C.; Oliva, A.L.; Arias, A.H.; Orazi, M.M.; Marcovecchio, J.E.
Filiación:Instituto Argentino de Oceanografía (IADO, CONICET/UNS), Camino La Carrindanga km 7.5, Bahía Blanca, 8000, Argentina
Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, San Juan 670, Bahía Blanca, 8000, Argentina
Departamento de Química, Area III, Química Analítica, Universidad Nacional del Sur, Av Alem 1253, Bahía Blanca, 8000, Argentina
Universidad de la Fraternidad de Agrupaciones Santo Tomás de Aquino, Gascón 3145, Mar del Plata, 7600, Argentina
Universidad Tecnológica Nacional, FRBB, 11 de Abril 445, Bahía Blanca, 8000, Argentina
Academia Nacional de Ciencias Exactas, Físicas y Naturales (ANCEFN), Av. Alvear 1711, 4to piso, Buenos Aires, 1014, Argentina
Palabras clave:Biomarkers; Fish; Polycyclic aromatic hydrocarbons; biomarker; environmental impact; enzyme activity; fish; lipid; oxidation; oxidative stress; PAH; protein; Argentina; Bahia Blanca Estuary; Buenos Aires [Argentina]; Ramnogaster arcuata
Año:2019
Volumen:13
Número:1
Página de inicio:77
Página de fin:89
DOI: http://dx.doi.org/10.1007/s41742-018-0155-2
Handle:http://hdl.handle.net/20.500.12110/paper_17356865_v13_n1_p77_Ronda
Título revista:International Journal of Environmental Research
Título revista abreviado:Int. J. Environ. Res.
ISSN:17356865
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_17356865_v13_n1_p77_Ronda

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Citas:

---------- APA ----------
Ronda, A.C., Oliva, A.L., Arias, A.H., Orazi, M.M. & Marcovecchio, J.E. (2019) . Biomarker Responses to Polycyclic Aromatic Hydrocarbons in the Native Fish Ramnogaster arcuata, South America. International Journal of Environmental Research, 13(1), 77-89.
http://dx.doi.org/10.1007/s41742-018-0155-2
---------- CHICAGO ----------
Ronda, A.C., Oliva, A.L., Arias, A.H., Orazi, M.M., Marcovecchio, J.E. "Biomarker Responses to Polycyclic Aromatic Hydrocarbons in the Native Fish Ramnogaster arcuata, South America" . International Journal of Environmental Research 13, no. 1 (2019) : 77-89.
http://dx.doi.org/10.1007/s41742-018-0155-2
---------- MLA ----------
Ronda, A.C., Oliva, A.L., Arias, A.H., Orazi, M.M., Marcovecchio, J.E. "Biomarker Responses to Polycyclic Aromatic Hydrocarbons in the Native Fish Ramnogaster arcuata, South America" . International Journal of Environmental Research, vol. 13, no. 1, 2019, pp. 77-89.
http://dx.doi.org/10.1007/s41742-018-0155-2
---------- VANCOUVER ----------
Ronda, A.C., Oliva, A.L., Arias, A.H., Orazi, M.M., Marcovecchio, J.E. Biomarker Responses to Polycyclic Aromatic Hydrocarbons in the Native Fish Ramnogaster arcuata, South America. Int. J. Environ. Res. 2019;13(1):77-89.
http://dx.doi.org/10.1007/s41742-018-0155-2