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Guerra, L.N.; Suarez, C.; Soto, D.; Schiappacasse, A.; Sapochnik, D.; Sacca, P.; Piwien-Pilipuk, G.; Peral, B.; Calvo, J.C. "GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes" (2015) Clinical and Translational Oncology. 17(7):511-520
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Abstract:

Introduction: In the mammary gland, the involution that occurs when lactation ends is an important period for cancer development. We have previously demonstrated stromal–epithelium interactions evaluating conditioned medium of adipose tissue on breast epithelial metalloproteases activity (Creydt et al., Clin Transl Oncol 15:124–131, 2013). Here, we evaluated the effects of conditioned medium of breast epithelial mammary cells on stromal cells. Materials and methods: Conditioned medium from normal murine mammary gland cell line (NMuMG) and conditioned medium proteins were obtained. Then, they were evaluated on modulation of adipocyte differentiation, using 3T3-L1 cell line. Results: We described, for the first time, that breast epithelial mammary cells could produce the enzyme galactose 3-O-sulfotransferase 2 (GAL3ST2). Importantly, GAL3ST2 is present in NMMuMG and two human breast cancer cell lines, and it is more strongly expressed in more metastatic tumors. When 3T3-L1 preadipocyte differentiation was triggered in the presence of conditioned medium from NMuMG or GAL3ST2, triglyceride accumulation was decreased by 40 % and C/EBPβ expression by 80 % in adipocytes. In addition, the expression of FABP4 (aP2), another marker of adipocyte differentiation, was inhibited by 40 % in GAL3ST2-treated cells. Conclusions: Taken together, these results suggest that GAL3ST2 would interfere with normal differentiation of 3T3-L1 preadipocytes; raising the possibility that it may affect normal differentiation of stromal preadipocytes and be a link to tumor metastatic capacity. © 2014, Federación de Sociedades Españolas de Oncología (FESEO).

Registro:

Documento: Artículo
Título:GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes
Autor:Guerra, L.N.; Suarez, C.; Soto, D.; Schiappacasse, A.; Sapochnik, D.; Sacca, P.; Piwien-Pilipuk, G.; Peral, B.; Calvo, J.C.
Filiación:Departamento de Química Biológica, Universidad de Buenos Aires—IQUIBICEN, CONICET, Intendente Güiraldes 2160, Pabellón 2, Buenos Aires, 1428, Argentina
Departamento de Computación, Universidad de Buenos Aires, Intendente Güiraldes 2160, Pabellón 1, Buenos Aires, 1428, Argentina
Laboratorio de Proteoglicanos y Matriz extracelular, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Laboratorio de Arquitectura nuclear, Instituto de Biología y Medicina Experimental (IByME), CONICET, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Instituto de Investigaciones Biomédicas, Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC), Universidad Autónoma de Madrid, (CSIC, UAM), Madrid, 28029, Spain
Palabras clave:Adipocytes; Breast mammary gland; Cancer; Cell lines; Triglycerides; enzyme; galactose 3 O sulfotransferase 2; triacylglycerol; unclassified drug; CCAAT enhancer binding protein beta; Fabp4 protein, mouse; fatty acid binding protein; GAL3ST2 protein, human; Gal3St2 protein, mouse; sulfotransferase; sulfurtransferase; triacylglycerol; adipocyte; animal cell; Article; breast epithelium; cell differentiation; column chromatography; controlled study; genetic transfection; human; human cell; matrix assisted laser desorption ionization time of flight mass spectrometry; mouse; MTT assay; nonhuman; protein analysis; protein expression; two dimensional electrophoresis; Western blotting; 3T3 cell line; adipocyte; adipogenesis; animal; cell differentiation; cytology; female; HEK293 cell line; mass spectrometry; MCF 7 cell line; metabolism; tumor cell line; udder; 3T3-L1 Cells; Adipocytes; Adipogenesis; Animals; CCAAT-Enhancer-Binding Protein-beta; Cell Differentiation; Cell Line, Tumor; Fatty Acid-Binding Proteins; Female; HEK293 Cells; Humans; Mammary Glands, Animal; MCF-7 Cells; Mice; NIH 3T3 Cells; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfotransferases; Sulfurtransferases; Triglycerides
Año:2015
Volumen:17
Número:7
Página de inicio:511
Página de fin:520
DOI: http://dx.doi.org/10.1007/s12094-014-1267-6
Título revista:Clinical and Translational Oncology
Título revista abreviado:Clin. Transl. Oncol.
ISSN:1699048X
CAS:sulfotransferase, 9023-09-0; sulfurtransferase, 9023-05-6; CCAAT-Enhancer-Binding Protein-beta; Fabp4 protein, mouse; Fatty Acid-Binding Proteins; GAL3ST2 protein, human; Gal3St2 protein, mouse; Sulfotransferases; Sulfurtransferases; Triglycerides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v17_n7_p511_Guerra

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Citas:

---------- APA ----------
Guerra, L.N., Suarez, C., Soto, D., Schiappacasse, A., Sapochnik, D., Sacca, P., Piwien-Pilipuk, G.,..., Calvo, J.C. (2015) . GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes. Clinical and Translational Oncology, 17(7), 511-520.
http://dx.doi.org/10.1007/s12094-014-1267-6
---------- CHICAGO ----------
Guerra, L.N., Suarez, C., Soto, D., Schiappacasse, A., Sapochnik, D., Sacca, P., et al. "GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes" . Clinical and Translational Oncology 17, no. 7 (2015) : 511-520.
http://dx.doi.org/10.1007/s12094-014-1267-6
---------- MLA ----------
Guerra, L.N., Suarez, C., Soto, D., Schiappacasse, A., Sapochnik, D., Sacca, P., et al. "GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes" . Clinical and Translational Oncology, vol. 17, no. 7, 2015, pp. 511-520.
http://dx.doi.org/10.1007/s12094-014-1267-6
---------- VANCOUVER ----------
Guerra, L.N., Suarez, C., Soto, D., Schiappacasse, A., Sapochnik, D., Sacca, P., et al. GAL3ST2 from mammary gland epithelial cells affects differentiation of 3T3-L1 preadipocytes. Clin. Transl. Oncol. 2015;17(7):511-520.
http://dx.doi.org/10.1007/s12094-014-1267-6