Navegar

Colección

Datos Estadísticas

Artículo

Pistone Creydt, V.; Fletcher, S.J.; Giudice, J.; Bruzzone, A.; Chasseing, N.A.; Gonzalez, E.G.; Sacca, P.A.; Calvo, J.C. "Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells" (2013) Clinical and Translational Oncology. 15(2):124-131
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Introduction: Stromal-epithelial interactions mediate both breast development and breast cancer progression. In the present work, we evaluated the effects of conditioned media (CMs) of human adipose tissue explants from normal (hATN) and tumor (hATT) breast on proliferation, adhesion, migration and metalloproteases activity on tumor (MCF-7 and IBH-7) and non-tumor (MCF-10A) human breast epithelial cell lines. Materials and methods: Human adipose tissues were obtained from patients and the conditioned medium from hATN and hATT collected after 24 h of incubation. MCF-10A, MCF-7 and IBH-7 cells were grown and incubated with CMs and proliferation and adhesion, as well as migration ability and metalloprotease activity, of epithelial cells after exposing cell cultures to hATN- or hATT-CMs were quantified. The statistical significance between different experimental conditions was evaluated by one-way ANOVA. Tukey′s post hoc tests were performed. Results: Tumor and non-tumor breast epithelial cells significantly increased their proliferation activity after 24 h of treatment with hATT-CMs compared to control-CMs. Furthermore, cellular adhesion of these two tumor cell lines was significantly lower with hATT-CMs than with hATN-CMs. Therefore, hATT-CMs seem to induce significantly lower expression or less activity of the components involved in cellular adhesion than hATN-CMs. In addition, hATT-CMs induced pro-MMP-9 and MMP-9 activity and increased the migration of MCF-7 and IBH-7 cells compared to hATN-CMs. Conclusions: We conclude that the microenvironment of the tumor interacts in a dynamic way with the mutated epithelium. This evidence leads to the possibility to modify the tumor behavior/phenotype through the regulation or modification of its microenvironment. We developed a model in which we obtained CMs from adipose tissue explants completely, either from normal or tumor breast. In this way, we studied the contribution of soluble factors independently of the possible effects of direct cell contact. © 2012 Federación de Sociedades Españolas de Oncología (FESEO).

Registro:

Documento: Artículo
Título:Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells
Autor:Pistone Creydt, V.; Fletcher, S.J.; Giudice, J.; Bruzzone, A.; Chasseing, N.A.; Gonzalez, E.G.; Sacca, P.A.; Calvo, J.C.
Filiación:Instituto de Biología y Medicina Experimental (IByME), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Vuelta de Obligado 2490 C1428DN, Buenos Aires, Argentina
Instituto de Oncología Ángel H. Roffo, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Breast epithelial cells; Cancer; Epithelial-stromal interactions; Human breast adipose tissue; gelatinase B; adipose tissue; article; breast epithelium; breast tumor; cell adhesion; cell migration; cell proliferation; controlled study; enzyme activity; enzyme assay; explant; human; human cell; human tissue; molecular dynamics; quantitative analysis; tissue characterization; tissue interaction; tissue level; tumor growth; Adipose Tissue; Breast Neoplasms; Cell Adhesion; Cell Movement; Cell Proliferation; Culture Media, Conditioned; Epithelial Cells; Female; Humans; Mammary Glands, Human; Tumor Microenvironment
Año:2013
Volumen:15
Número:2
Página de inicio:124
Página de fin:131
DOI: http://dx.doi.org/10.1007/s12094-012-0896-x
Título revista:Clinical and Translational Oncology
Título revista abreviado:Clin. Transl. Oncol.
ISSN:1699048X
CAS:gelatinase B, 146480-36-6; Culture Media, Conditioned
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1699048X_v15_n2_p124_PistoneCreydt

Referencias:

  • Hennighausen, L., Robinson, G.W., Information networks in the mammary gland (2005) Nat Rev Mol Cell Biol, 6, pp. 715-725. , 16231422 10.1038/nrm1714 1:CAS:528:DC%2BD2MXhtVSrsb%2FI
  • Iyengar, P., Espina, V., Williams, T.W., Lin, Y., Berry, D., Jelicks, L.A., Lee, H., Scherer, P.E., Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment (2005) J Clin Invest, 115, pp. 1163-1176. , 15841211 1:CAS:528:DC%2BD2MXktFSktrY%3D
  • Krause, S., Maffini, M.V., Soto, A.M., Sonnenschein, C., The microenvironment determines the breast cancer cells' phenotype: Organization of MCF7 cells in 3D cultures (2010) BMC Cancer, 10, pp. 263-275. , 20529269 10.1186/1471-2407-10-263
  • Wu, Y., Kim, J.Y., Zhou, S., Smas, C.M., Differential screening identifies transcripts with depot-dependent expression in white adipose tissues (2008) BMC Genomics, 9, p. 397. , 18721461 10.1186/1471-2164-9-397
  • Tan, J., Buache, E., Chenard, M.P., Dali-Youcef, N., Rio, M.C., Adipocyte is a non-trivial, dynamic partner of breast cancer cells (2011) Int J Dev Biol, 55, pp. 851-859. , 21948738 10.1387/ijdb.113365jt
  • Dirat, B., Bochet, L., Dabek, M., Daviaud, D., Dauvillier, S., Majed, B., Wang, Y.Y., Muller, C., Cancer-associated adipocytes exhibit an activated phenotype and contribute to breast cancer invasion (2011) Cancer Res, 71, pp. 2455-2465. , 21459803 10.1158/0008-5472.CAN-10-3323 1:CAS:528:DC%2BC3MXktVOjt7k%3D
  • Walter, M., Liang, S., Ghosh, S., Hornsby, P.J., Li, R., Interleukin 6 secreted from adipose stromal cells promotes migration and invasion of breast cancer cells (2009) Oncogene, 28, pp. 2745-2755. , 19483720 10.1038/onc.2009.130 1:CAS:528:DC%2BD1MXms1alur4%3D
  • Rajala, M.W., Scherer, P.E., Minireview: The adipocyte-at the crossroads of energy homeostasis, inflammation, and atherosclerosis (2003) Endocrinology, 144, pp. 3765-3773. , 12933646 10.1210/en.2003-0580 1:CAS:528:DC%2BD3sXmvFWkurg%3D
  • Vona-Davis, L., Rose, D.P., Adipokines as endocrine, paracrine, and autocrine factors in breast cancer risk and progression (2007) Endocr Relat Cancer, 14, pp. 189-206. , 17639037 10.1677/ERC-06-0068 1:CAS:528:DC%2BD2sXhtV2lsr%2FE
  • Razmkhah, M., Jaberipour, M., Erfani, N., Habibagahi, M., Talei, A.R., Ghaderi, A., Adipose derived stem cells (ASCs) isolated from breast cancer tissue express IL-4, IL-10 and TGF-β1 and upregulate expression of regulatory molecules on T cells: Do they protect breast cancer cells from the immune response? (2011) Cell Immunol, 266, pp. 116-122. , 20970781 10.1016/j.cellimm.2010.09.005 1:CAS:528:DC%2BC3cXhsFGgs7nE
  • Jemal, A., Siegel, R., Xu, J., Ward, E., Cancer statistics, 2010 (2010) CA Cancer J Clin, 60, pp. 277-300. , 20610543 10.3322/caac.20073
  • Joyce, J.A., Pollard, J.W., Microenvironmental regulation of metastasis (2009) Nat Rev Cancer, 9, pp. 239-252. , 19279573 10.1038/nrc2618 1:CAS:528:DC%2BD1MXivFCjsLg%3D
  • Egeblad, M., Werb, Z., New functions for the matrix metalloproteinases in cancer progression (2002) Nat Rev Cancer, 2, pp. 161-174. , 11990853 10.1038/nrc745 1:CAS:528:DC%2BD38Xis1KktL8%3D
  • Pistone Creydt, V., Sacca, P.A., Tesone, A.J., Vidal, L., Calvo, J.C., Adipocyte differentiation influences the proliferation and migration of normal and tumoral breast epithelial cells (2010) Mol Med Report, 3, pp. 433-439
  • Maffini, M.V., Soto, A.M., Calabro, J.M., Ucci, A.A., Sonnenschein, C., The stroma as a crucial target in rat mammary gland carcinogenesis (2004) J Cell Sci, 117, pp. 1495-1502. , 14996910 10.1242/jcs.01000 1:CAS:528:DC%2BD2cXjs1ygu70%3D
  • Maffini, M.V., Calabro, J.M., Soto, A.M., Sonnenschein, C., Stromal regulation of neoplastic development. Age-dependent normalization of neoplastic mammary cells by mammary stroma (2005) Am J Pathol, 167, pp. 1405-1410. , 16251424 10.1016/S0002-9440(10)61227-8
  • Baker, S.G., Cappuccio, A., Potter, J.D., Research on early-stage carcinogenesis: Are we approaching paradigm instability? (2010) J Clin Oncol, 28, pp. 3215-3218. , 20547997 10.1200/JCO.2010.28.5460
  • Zangani, D., Darcy, K.M., Shoemaker, S., Ip, M.M., Adipocyte-epithelial interactions regulate the in vitro development of normal mammary epithelial cells (1999) Exp Cell Res, 247, pp. 399-409. , 10066368 10.1006/excr.1998.4373 1:CAS:528:DyaK1MXhsVCltrg%3D
  • Rahimi, N., Saulnier, R., Nakamura, T., Park, M., Elliott, B., Role of hepatocyte growth factor in breast cancer: A novel mitogenic factor secreted by adipocytes (1994) DNA Cell Biol, 13, pp. 1189-1197. , 7811385 10.1089/dna.1994.13.1189 1:CAS:528:DyaK2MXivF2htLY%3D
  • Pinilla, S., Alt, E., Abdul Khalek, F.J., Jotzu, C., Muehlberg, F., Beckmann, C., Song, Y.H., Tissue resident stem cells produce CCL5 under the influence of cancer cells and thereby promote breast cancer cell invasion (2009) Cancer Lett, 284, pp. 80-85. , 19427114 10.1016/j.canlet.2009.04.013 1:CAS:528:DC%2BD1MXhtVKlsLnE
  • Dirat, B., Bochet, L., Escourrou, G., Valet, P., Muller, C., Unraveling the obesity and breast cancer links: A role for cancer-associated adipocytes? (2010) Endocr Dev, 19, pp. 45-52. , 20551667 10.1159/000316896
  • Hugo, E.R., Brandebourg, T.D., Woo, J.G., Loftus, J., Alexander, J.W., Ben-Jonathan, N., Bisphenol A at environmentally relevant doses inhibits adiponectin release from human adipose tissue explants and adipocytes (2008) Environ Health Perspect, 116, pp. 1642-1647. , 19079714 10.1289/ehp.11537 1:CAS:528:DC%2BD1MXmtVCkug%3D%3D
  • Borcherding, D.C., Hugo, E.R., Idelman, G., De Silva, A., Richtand, N.W., Loftus, J., Ben-Jonathan, N., Dopamine receptors in human adipocytes: Expression and functions (2011) PLoS One, 6, p. 25537. , 21966540 10.1371/journal.pone.0025537 1:CAS:528:DC%2BC3MXhtleisLnO
  • Al-Lahham, S., Roelofsen, H., Rezaee, F., Weening, D., Hoek, A., Vonk, R., Venema, K., Propionic acid affects immune status and metabolism in adipose tissue from overweight subjects (2012) Eur J Clin Invest, 42, pp. 357-364. , 21913915 10.1111/j.1365-2362.2011.02590.x 1:CAS:528:DC%2BC38Xms1Wlsrc%3D
  • Vázquez, S.M., Mladovan, A., Garbovesky, C., Baldi, A., Lüthy, I.A., Three novel hormone-responsive cell lines derived from primary human breast carcinomas: Functional characterization (2004) J Cell Physiol, 199, pp. 460-469. , 15095293 10.1002/jcp.10466
  • Bruzzone, A., Vanzulli, S.I., Soldati, R., Giulianelli, S., Lanari, C., Lüthy, I.A., Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice (2009) J Cell Physiol, 219, pp. 477-484. , 19194992 10.1002/jcp.21694 1:CAS:528:DC%2BD1MXktVWjsbo%3D
  • Retta, S.F., Ternullo, M., Tarone, G., Adhesion to matrix proteins (1999) Adhesion Protein Protocols, pp. 125-130. , Dejana E, Corada M (eds) Humana Press INC., Totowa
  • Casey, T., Bond, J., Tighe, S., Hunter, T., Lintault, L., Patel, O., Eneman, J., Plaut, K., Molecular signatures suggest a major role for stromal cells in development of invasive breast cancer (2009) Breast Cancer Res Treat, 114, pp. 47-62. , 18373191 10.1007/s10549-008-9982-8 1:CAS:528:DC%2BD1MXhtVehsL0%3D
  • Trimboli, A.J., Cantemir-Stone, C.Z., Li, F., Wallace, J.A., Merchant, A., Creasap, N., Thompson, J.C., Leone, G., Pten in stromal fibroblasts suppresses mammary epithelial tumours (2009) Nature, 461, pp. 1084-1091. , 19847259 10.1038/nature08486 1:CAS:528:DC%2BD1MXhtlKgsrbK
  • Sacca, P.A., Pistone Creydt, V., Tesone, A.J., Calvo, J.C., A novel and alternative in vitro method using microwave to study the epithelial-stromal interactions (2009) Sci Res Essays, 4, pp. 1487-1495

Citas:

---------- APA ----------
Pistone Creydt, V., Fletcher, S.J., Giudice, J., Bruzzone, A., Chasseing, N.A., Gonzalez, E.G., Sacca, P.A.,..., Calvo, J.C. (2013) . Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells. Clinical and Translational Oncology, 15(2), 124-131.
http://dx.doi.org/10.1007/s12094-012-0896-x
---------- CHICAGO ----------
Pistone Creydt, V., Fletcher, S.J., Giudice, J., Bruzzone, A., Chasseing, N.A., Gonzalez, E.G., et al. "Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells" . Clinical and Translational Oncology 15, no. 2 (2013) : 124-131.
http://dx.doi.org/10.1007/s12094-012-0896-x
---------- MLA ----------
Pistone Creydt, V., Fletcher, S.J., Giudice, J., Bruzzone, A., Chasseing, N.A., Gonzalez, E.G., et al. "Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells" . Clinical and Translational Oncology, vol. 15, no. 2, 2013, pp. 124-131.
http://dx.doi.org/10.1007/s12094-012-0896-x
---------- VANCOUVER ----------
Pistone Creydt, V., Fletcher, S.J., Giudice, J., Bruzzone, A., Chasseing, N.A., Gonzalez, E.G., et al. Human adipose tissue from normal and tumoral breast regulates the behavior of mammary epithelial cells. Clin. Transl. Oncol. 2013;15(2):124-131.
http://dx.doi.org/10.1007/s12094-012-0896-x