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Abstract:

Human cystic echinococcosis remains a major public health problem on several countries and the treatment strategies are not solved. The aim of the present work was to determine the in vitro effect of thymol and Mentha piperita, M. pulegium, and Rosmarinus officinalis essential oils on the proliferation of E. granulosus larval cells. Isolated cells and cellular aggregates were obtained from hydatid cyst's germinal layer and exposed to 1, 5, and 10 g/ml of thymol and the different essential oils for 7 days. Drug effect was evaluated using test viability and scanning electron microscopy. Control cell culture viability was 2.1 x 106 (100%) after 7 days of incubation. At day 7, thymol 5 g/ml caused a reduction in cell viability of 63% and the essential oils of M. piperita 10 g/ml, M. pulegium 10 g/ml, and R. officinalis 10 g/ml produced a reduction in the viability of 77, 82, and 71%, respectively. Moreover essential oils caused reduction in cell number, collapsed cells, and loss of normal tridimensional composition of the aggregates. Due to the inhibitory effect caused by essential oils on E. granulosus cells we suggested that it would be an effective means for suppression of larval growth. © 2014 Clara María Albani et al.

Registro:

Documento: Artículo
Título:Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells
Autor:Albani, C.M.; Denegri, G.M.; Elissondo, M.C.
Filiación:Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar Del Plata (UNMdP), Funes 3350, Mar del Plata, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
Palabras clave:anthelmintic agent; essential oil; thymol; anthelmintic activity; Article; cell count; cell proliferation; cell survival; cell viability; controlled study; drug effect; drug mechanism; Echinococcus granulosus; in vitro study; Mentha piperita; Mentha pulegium; nonhuman; rosemary; scanning electron microscopy
Año:2014
Volumen:2014
DOI: http://dx.doi.org/10.1155/2014/746931
Título revista:Interdisciplinary Perspectives on Infectious Diseases
Título revista abreviado:Interdiscip. Perspect. Infect. Dis.
ISSN:1687708X
CAS:thymol, 89-83-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1687708X_v2014_n_p_Albani

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Citas:

---------- APA ----------
Albani, C.M., Denegri, G.M. & Elissondo, M.C. (2014) . Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells. Interdisciplinary Perspectives on Infectious Diseases, 2014.
http://dx.doi.org/10.1155/2014/746931
---------- CHICAGO ----------
Albani, C.M., Denegri, G.M., Elissondo, M.C. "Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells" . Interdisciplinary Perspectives on Infectious Diseases 2014 (2014).
http://dx.doi.org/10.1155/2014/746931
---------- MLA ----------
Albani, C.M., Denegri, G.M., Elissondo, M.C. "Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells" . Interdisciplinary Perspectives on Infectious Diseases, vol. 2014, 2014.
http://dx.doi.org/10.1155/2014/746931
---------- VANCOUVER ----------
Albani, C.M., Denegri, G.M., Elissondo, M.C. Effect of different terpene-containing essential oils on the proliferation of echinococcus granulosus larval cells. Interdiscip. Perspect. Infect. Dis. 2014;2014.
http://dx.doi.org/10.1155/2014/746931