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Abstract:

Background: Glycosidases profusion in male reproductive fluids suggests a possible relationship with sperm function. Although Hexosaminidase (Hex) is the most active glycosidase in epididymal fluid and seminal plasma, as well as in spermatozoa, Glucosidase is considered a marker for epididymal function and azoospermia. Objective: The aim of this study was to determine Hex activity in seminal plasma from patients with normal and abnormal spermograms and analyze its correlation with seminal parameters. Materials and Methods: In this cross sectional study, seminal plasma from azoospermic, asthenozoospermic, teratozoospermic, and normozoospermic patients was analyzed for the activity of: total Hex, HexA isoform, and glucosidase. Besides, hexosamine levels were determined, and the amount of Hex was quantified by immunoblot with a specific antibody. Correlation of Hex activity with seminal parameters was also analyzed. Results: Hex activity, like glucosidase, was significantly reduced in azoospermic samples (44, 49, and 60% reduction for total Hex, HexA and glucosidase, respectively). A reduced amount of Hex in azoospermic samples was confirmed by western immunoblot. Hex activity was negatively correlated with round cells in azoospermic samples and positively correlated with motility in asthenozoospermic ones. Conclusion: The results suggested that Hex activity was reduced in azoospermic samples and this was due to a lower amount of enzyme. The correlation to seminal parameters related to particular pathologies suggests a possible relationship of Hex with fertilizing capacity. © 2015 Research and Clinical Center for Infertitlity. All rights reserved.

Registro:

Documento: Artículo
Título:Seminal plasma hexosaminidase in patients with normal and abnormal spermograms
Autor:Menendez-Helman, R.J.; Sanjurjo, C.; Miranda, P.V.
Filiación:Instituto de Biología y Medicina Experimental – CONICET, Buenos Aires, Argentina
IQUIBICEN (FCEyN, UBA – CONICET). Buenos Aires, Argentina
Fertilab, Buenos Aires, Argentina
Instituto de Agrobiotecnología Rosario (INDEAR), Santa Fe, Argentina
Palabras clave:Glycosidases; Hexosaminidase; N-acetylglucosaminidase; Semen Analysis; Seminal plasma; beta n acetylhexosaminidase; beta n acetylhexosaminidase A; glucosidase; glutamic acid; peroxidase; phosphatase; antibody specificity; Article; asthenospermia; azoospermia; cell motility; cell structure; cells by body anatomy; controlled study; correlation analysis; cross-sectional study; disease classification; enzyme activity; fertilization; human; human cell; human tissue; immunoblotting; male; protein expression; round cell; semen analysis; seminal plasma; spermatozoon abnormality; spermiogram; Western blotting; asthenospermia; azoospermia; enzyme activity; fluorometry; polyacrylamide gel electrophoresis; semen analysis; seminal plasma; spermatozoon density; spermatozoon motility
Año:2015
Volumen:13
Número:9
Página de inicio:541
Página de fin:548
Título revista:Iranian Journal of Reproductive Medicine
Título revista abreviado:Iran. J. Reprod. Med.
ISSN:16806433
CAS:beta n acetylhexosaminidase, 37211-57-7, 9012-33-3, 9027-52-5; glucosidase, 9033-06-1; glutamic acid, 11070-68-1, 138-15-8, 56-86-0, 6899-05-4; peroxidase, 9003-99-0; phosphatase, 9013-05-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman

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Citas:

---------- APA ----------
Menendez-Helman, R.J., Sanjurjo, C. & Miranda, P.V. (2015) . Seminal plasma hexosaminidase in patients with normal and abnormal spermograms. Iranian Journal of Reproductive Medicine, 13(9), 541-548.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman [ ]
---------- CHICAGO ----------
Menendez-Helman, R.J., Sanjurjo, C., Miranda, P.V. "Seminal plasma hexosaminidase in patients with normal and abnormal spermograms" . Iranian Journal of Reproductive Medicine 13, no. 9 (2015) : 541-548.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman [ ]
---------- MLA ----------
Menendez-Helman, R.J., Sanjurjo, C., Miranda, P.V. "Seminal plasma hexosaminidase in patients with normal and abnormal spermograms" . Iranian Journal of Reproductive Medicine, vol. 13, no. 9, 2015, pp. 541-548.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman [ ]
---------- VANCOUVER ----------
Menendez-Helman, R.J., Sanjurjo, C., Miranda, P.V. Seminal plasma hexosaminidase in patients with normal and abnormal spermograms. Iran. J. Reprod. Med. 2015;13(9):541-548.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16806433_v13_n9_p541_MenendezHelman [ ]