Artículo

Bustillo, S.; Lucero, H.; Leiva, L.C.; Acosta, O.; Kier Joffé, E.B.; Gorodner, J.O. "Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina" (2009) Journal of Venomous Animals and Toxins Including Tropical Diseases. 15(1):28-42
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Abstract:

Bothrops snake venoms have been proved toxic to a variety of cell types, in both in vivo and in vitro models. Studies on the pharmacological actions of Bothrops venoms from Argentina are relatively scarce and the direct action of the crude venoms has not been assessed using cell culture models. In this work, we investigated the cytotoxicity of crude venoms from B. alternatus and B. diporus in a skeletal muscle (C2C12) cell line, which is commonly used as a model for studying the myotoxic action of snake venom. Both venoms (1.25-50 μg/mL) induced an early and significant decrease in cell viability. The cytotoxic concentration 50 (CC 50), determined three hours after exposure, revealed that B. diporus venom was significantly more cytotoxic (CC 50: 2 μg/mL) than B. alternatus (CC 50: 5.8 μg/mL). To investigate the cell death mechanism involved, myoblast cells were examined by phase contrast microscopy and after acridine orange and ethidium bromide fluorescence staining, respectively. Our data clearly demonstrated that an apoptotic mechanism mediated this cell line destruction. The current study aimed to provide new information on the cytotoxicity mechanisms of Argentine Bothrops snake venoms on a skeletal muscle cell line.

Registro:

Documento: Artículo
Título:Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina
Autor:Bustillo, S.; Lucero, H.; Leiva, L.C.; Acosta, O.; Kier Joffé, E.B.; Gorodner, J.O.
Filiación:Tissue Culture Sector, Regional Institute of Medicine, Northeast National University, Corrientes, Argentina
Laboratory of Biological Biochemistry, Northeast National University, Corrientes, Argentina
Laboratory of Pharmacology, Northeast National University, Corrientes, Argentina
Ángel H. Roffo Oncology Institute, University of Buenos Aires, Buenos Aires, Argentina
Facultad de Ciencias Exactas Y Naturales Y Agrimensura, Campus Deodoro Roca, Av. Libertad, 5450, (3400) Corrientes, Argentina
Idioma: Inglés
Palabras clave:Apoptosis; Bothrops alternatus; Bothrops diporus; C2C12; Cytotoxicity; acridine orange; Bothrops alternatus venom; Bothrops diporus venom; ethidium bromide; fluorescent dye; snake venom; unclassified drug; animal cell; apoptosis; Argentina; article; cell death; cell structure; cell viability; concentration (parameters); controlled study; cytotoxicity; mouse; muscle cell; myoblast; nonhuman; phase contrast microscopy; skeletal muscle; snake; Bothrops; Bothrops alternatus
Año:2009
Volumen:15
Número:1
Página de inicio:28
Página de fin:42
DOI: http://dx.doi.org/10.1590/S1678-91992009000100004
Título revista:Journal of Venomous Animals and Toxins Including Tropical Diseases
Título revista abreviado:J. Venomous Anim. Toxins Incl. Trop. Dis.
ISSN:16789199
CAS:acridine orange, 494-38-2, 65-61-2; ethidium bromide, 1239-45-8; snake venom, 55230-69-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16789199_v15_n1_p28_Bustillo

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Citas:

---------- APA ----------
Bustillo, S., Lucero, H., Leiva, L.C., Acosta, O., Kier Joffé, E.B. & Gorodner, J.O. (2009) . Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina. Journal of Venomous Animals and Toxins Including Tropical Diseases, 15(1), 28-42.
http://dx.doi.org/10.1590/S1678-91992009000100004
---------- CHICAGO ----------
Bustillo, S., Lucero, H., Leiva, L.C., Acosta, O., Kier Joffé, E.B., Gorodner, J.O. "Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina" . Journal of Venomous Animals and Toxins Including Tropical Diseases 15, no. 1 (2009) : 28-42.
http://dx.doi.org/10.1590/S1678-91992009000100004
---------- MLA ----------
Bustillo, S., Lucero, H., Leiva, L.C., Acosta, O., Kier Joffé, E.B., Gorodner, J.O. "Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina" . Journal of Venomous Animals and Toxins Including Tropical Diseases, vol. 15, no. 1, 2009, pp. 28-42.
http://dx.doi.org/10.1590/S1678-91992009000100004
---------- VANCOUVER ----------
Bustillo, S., Lucero, H., Leiva, L.C., Acosta, O., Kier Joffé, E.B., Gorodner, J.O. Cytotoxicity and morphological analysis of cell death induced by Bothrops venoms from the northeast of Argentina. J. Venomous Anim. Toxins Incl. Trop. Dis. 2009;15(1):28-42.
http://dx.doi.org/10.1590/S1678-91992009000100004