Artículo

da Costa, T.V.; Oshiro, L.M.Y.; de Mattos, L.A.; López Greco, L.S.; Melo, E.P.; Flor, H.R. "Toxicity, freezing and cold storage tests of native species semen " (2017) Boletim do Instituto de Pesca. 43(3):334-346
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Abstract:

In the first part of this study, toxicity tests were performed on the sperm of Macrobrachium acanthurus using four cryoprotectants for periods of 10 and 20 min at concentrations of 10 and 20%. In the second part, cryopreservation was performed by applying the least toxic cryoprotectant, and two freezing methods were tested over 24 hours: automated (protocols A and B) and conventional (protocols C and D). Protocol A exhibited a cooling rate of 0.5°C min-1 from -6°C to -32°C; protocol B was similar to A except for the starting temperature, which was room temperature; whereas protocols C and D exhibited a cooling rate of 2 and 10°C min-1, respectively. The third part of the study was conducted to assess the lifespan of the sperm when stored at 5°C, in which sperm viability was evaluated by a semen smear with eosin-nigrosin. The least toxic cryoprotectants were 10 and 20% glycerol, and 10% methanol, and the equilibrium time was 10 minutes. The optimal cooling rate was 2°C min-1 for 10% glycerol, which had a sperm survival rate of 21.8%. Cold storage for up to 3 days is recommended, presenting a sperm survival rate of 35.3%. © 2017, Instytut Technologii Drewna. All Rights reserved.

Registro:

Documento: Artículo
Título:Toxicity, freezing and cold storage tests of native species semen
Autor:da Costa, T.V.; Oshiro, L.M.Y.; de Mattos, L.A.; López Greco, L.S.; Melo, E.P.; Flor, H.R.
Filiación:Universidade Federal do Amazonas, Instituto de Ciências Sociais, Educação e Zootecnia, Estrada Parintins/Macurany, 1805, Jacareacanga, Parintins, AM 152-240, Brazil
Universidade Federal Rural do Rio de Janeiro, Estação de Biologia Marinha, Rua Sereder, s/n, Itacuruçá, Mangaratiba, RJ 860-020, Brazil
Universidad de Buenos Aires, Departamento de Biodiversidad y Biología Experimental, FCEN e IBBEA, CONICET-UBA, Argentina
Palabras clave:Caridea; Cryopreservation; Cryoprotectants; Glycerol; Methanol; Shrimp
Año:2017
Volumen:43
Número:3
Página de inicio:334
Página de fin:346
DOI: http://dx.doi.org/10.20950/1678-2305.2017v43n3p334
Título revista:Boletim do Instituto de Pesca
Título revista abreviado:Bol. Insttituto Pesca
ISSN:16782305
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16782305_v43_n3_p334_daCosta

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Citas:

---------- APA ----------
da Costa, T.V., Oshiro, L.M.Y., de Mattos, L.A., López Greco, L.S., Melo, E.P. & Flor, H.R. (2017) . Toxicity, freezing and cold storage tests of native species semen . Boletim do Instituto de Pesca, 43(3), 334-346.
http://dx.doi.org/10.20950/1678-2305.2017v43n3p334
---------- CHICAGO ----------
da Costa, T.V., Oshiro, L.M.Y., de Mattos, L.A., López Greco, L.S., Melo, E.P., Flor, H.R. "Toxicity, freezing and cold storage tests of native species semen " . Boletim do Instituto de Pesca 43, no. 3 (2017) : 334-346.
http://dx.doi.org/10.20950/1678-2305.2017v43n3p334
---------- MLA ----------
da Costa, T.V., Oshiro, L.M.Y., de Mattos, L.A., López Greco, L.S., Melo, E.P., Flor, H.R. "Toxicity, freezing and cold storage tests of native species semen " . Boletim do Instituto de Pesca, vol. 43, no. 3, 2017, pp. 334-346.
http://dx.doi.org/10.20950/1678-2305.2017v43n3p334
---------- VANCOUVER ----------
da Costa, T.V., Oshiro, L.M.Y., de Mattos, L.A., López Greco, L.S., Melo, E.P., Flor, H.R. Toxicity, freezing and cold storage tests of native species semen . Bol. Insttituto Pesca. 2017;43(3):334-346.
http://dx.doi.org/10.20950/1678-2305.2017v43n3p334