Artículo
Abstract:
Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome microdissection to develop a FISH probe of the chromosome 11 heterochromatic block (11qHe+) of Cebus apella paraguayanus (CAPp). Fragments of the 11qHe+ microdissected from fibroblast cell culture were collected in a PCR tube, amplified by degenerate oligonucleotide primer-PCR and subsequently labeled. The specificity of the FISH probe was confirmed in metaphases of some Ceboidea species. Signals were located in the He+ of chromosomes 4, 11, 12, 13, and 19 of CAPp and in the He+ of chromosomes 4, 12 and 13 of C. a. nigritus (CAPn); no signals were observed when other Ceboidea species were analyzed. We propose that the heterochromatin observed in CAPp and CAPn is specific for these species. We consider this C. apella heterochromatin identity as a possible key for the interpretation of chromosomal evolution in these Ceboidea.
Registro:
| Documento: | Artículo |
| Título: | Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. |
| Autor: | Nieves, M.; Mühlmann, M.; Mudry, M.D. |
| Filiación: | Grupo de Investigación en Biología Evolutiva (GIBE), Dpto. de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina |
| Palabras clave: | animal; article; Cebidae; chromosome banding pattern; female; fluorescence in situ hybridization; genetics; heterochromatin; male; methodology; microdissection; molecular evolution; polymerase chain reaction; sensitivity and specificity; Animals; Cebus; Chromosome Banding; Evolution, Molecular; Female; Heterochromatin; In Situ Hybridization, Fluorescence; Male; Microdissection; Polymerase Chain Reaction; Sensitivity and Specificity |
| Año: | 2005 |
| Volumen: | 4 |
| Número: | 4 |
| Página de inicio: | 675 |
| Página de fin: | 683 |
| Título revista: | Genetics and molecular research : GMR |
| Título revista abreviado: | Genet. Mol. Res. |
| ISSN: | 16765680 |
| CAS: | Heterochromatin |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16765680_v4_n4_p675_Nieves |
Citas:
---------- APA ----------
Nieves, M., Mühlmann, M. & Mudry, M.D. (2005) . Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. Genetics and molecular research : GMR, 4(4), 675-683.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16765680_v4_n4_p675_Nieves [ ]
---------- CHICAGO ----------
Nieves, M., Mühlmann, M., Mudry, M.D. "Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection." Genetics and molecular research : GMR 4, no. 4 (2005) : 675-683.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16765680_v4_n4_p675_Nieves [ ]
---------- MLA ----------
Nieves, M., Mühlmann, M., Mudry, M.D. "Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection." Genetics and molecular research : GMR, vol. 4, no. 4, 2005, pp. 675-683.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16765680_v4_n4_p675_Nieves [ ]
---------- VANCOUVER ----------
Nieves, M., Mühlmann, M., Mudry, M.D. Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection. Genet. Mol. Res. 2005;4(4):675-683.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16765680_v4_n4_p675_Nieves [ ]
