Artículo

Delgado-Cerezo, M.; Sánchez-Rodríguez, C.; Escudero, V.; Miedes, E.; Fernández, P.V.; Jordá, L.; Hernández-Blanco, C.; Sánchez-Vallet, A.; Bednarek, P.; Schulze-Lefert, P.; Somerville, S.; Estevez, J.M.; Persson, S.; Molina, A. "Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi" (2012) Molecular Plant. 5(1):98-114
Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγ subunits (γ1/AGG1 and γ2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulated genes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition. © 2011 The Author.

Registro:

Documento: Artículo
Título:Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi
Autor:Delgado-Cerezo, M.; Sánchez-Rodríguez, C.; Escudero, V.; Miedes, E.; Fernández, P.V.; Jordá, L.; Hernández-Blanco, C.; Sánchez-Vallet, A.; Bednarek, P.; Schulze-Lefert, P.; Somerville, S.; Estevez, J.M.; Persson, S.; Molina, A.
Filiación:Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Universidad Politécnica de Madrid, Campus de Montegancedo, E-28223-Pozuelo de Alarcón (Madrid), Spain
Max-Planck-Institute für Molekulare Pflanzenphysiologie, Am Mühlenberg 2, 14476 Potsdam, Germany
Laboratorio de Fisiología y Biología Molecular, IFIByNE (CONICET) FCEyN, Universidad de Buenos Aires, Pab. II, Intendente Güiraldes, 2160 Buenos Aires C1428EGA, Argentina
Laboratory of Phytopathology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, Netherlands
Max Planck Institut für Züchtungsforschung, Department of Plant Microbe Interactions, Carl-von-Linné-Weg 10, D-50829 Köln, Germany
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland
Energy Biosciences Institute, 130 Calvin Hall, University of California at Berkeley, Berkeley, CA 94720, United States
Palabras clave:cell wall; G-protein; indole glucosinolates; necrotropic fungi; Plant immunity; xylose; Arabidopsis; Fungi; Plectosphaerella cucumerina
Año:2012
Volumen:5
Número:1
Página de inicio:98
Página de fin:114
DOI: http://dx.doi.org/10.1093/mp/ssr082
Título revista:Molecular Plant
Título revista abreviado:Mol. Plant
ISSN:16742052
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16742052_v5_n1_p98_DelgadoCerezo

Referencias:

  • Adie, B.A.T., Perez-Perez, J., Perez-Perez, M.M., Godoy, M., Sanchez-Serrano, J.-J., Schmelz, E.A., Solano, R., ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis (2007) Plant Cell, 19 (5), pp. 1665-1681. , http://www.plantcell.org/cgi/reprint/19/5/1665.pdf, DOI 10.1105/tpc.106.048041
  • Adjobo-Hermans, M.J.W., Goedhart, J., Gadella Jr., T.W.J., Plant G protein heterotrimers require dual lipidation motifs of Gα and Gγ and do not dissociate upon activation (2006) Journal of Cell Science, 119 (24), pp. 5087-5097. , DOI 10.1242/jcs.03284
  • Bayer, E.M., Bottrill, A.R., Walshaw, J., Vigouroux, M., Naldrett, M.J., Thomas, C.L., Maule, A.J., Arabidopsis cell wall proteome defined using multidimensional protein identification technology (2006) Proteomics, 6 (1), pp. 301-311. , DOI 10.1002/pmic.200500046
  • Bednarek, P., A glucosinolate metabolism pathway in living plant cells mediates broad-spectrum antifungal defense (2009) Science., 323, pp. 101-106
  • Berrocal-Lobo, M., Molina, A., Solano, R., Constitutive expression of Ethylene-Response-Factor1 in arabidopsis confers resistance to several necrotrophic fungi (2002) Plant Journal, 29 (1), pp. 23-32. , DOI 10.1046/j.1365-313x.2002.01191.x
  • Bischoff, V., Cookson, S.J., Wu, S., Scheible, W.R., Thaxtomin A affects CESA-complex density, expression of cell wall genes, cell wall composition, and causes ectopic lignification in Arabidopsis thaliana seedlings (2009) J. Exp. Bot., 60, pp. 955-965
  • Blakeney, N.B., Harris, P.J., Henry, R.J., Stone, B.A., A simple and rapid preparation of alditol acetates for monosaccharide analysis (1983) Carbohydr. Res., 182, pp. 291-299
  • Bohman, S., Staal, J., Thomma, B.P.H.J., Wang, M., Dixelius, C., Characterisation of an Arabidopsis-Leptosphaeria maculans pathosystem: Resistance partially requires camalexin biosynthesis and is independent of salicylic acid, ethylene and jasmonic acid signalling (2004) Plant Journal, 37 (1), pp. 9-20. , DOI 10.1046/j.1365-313X.2003.01927.x
  • Brown, D.M., Zeef, L.A.H., Ellis, J., Goodacre, R., Turner, S.R., Identification of novel genes in Arabidopsis involved in secondary cell wall formation using expression profiling and reverse genetics (2005) Plant Cell, 17 (8), pp. 2281-2295. , DOI 10.1105/tpc.105.031542
  • Cantu, D., Vicente, A.R., Labavitch, J.M., Bennett, A.B., Powell, A.L., Strangers in the matrix: Plant cell walls and pathogen susceptibility (2008) Trends Plant Sci., 13, pp. 610-617
  • Chakravorty, D., Trusov, Y., Zhang, W., Acharya, B.R., Sheahan, M.B., McCurdy, D.W., Assmann, S.M., Botella, J.R., An atypical heterotrimeric G-protein c-subunit is involved in guard cell K+-channel regulation and morphological development in Arabidopsis thaliana (2011) Plant J., , doi:10.1111/j.1365-313X.2011.04638.x
  • Chen, J.-G., Willard, F.S., Huang, J., Liang, J., Chasse, S.A., Jones, A.M., Siderovski, D.P., A seven-transmembrane RGS protein that modulates plant cell proliferation (2003) Science, 301 (5640), pp. 1728-1731. , DOI 10.1126/science.1087790
  • Denby, K.J., Kumar, P., Kliebenstein, D.J., Identification of Botrytis cinerea susceptibility loci in Arabidopsis thaliana (2004) Plant Journal, 38 (3), pp. 473-486. , DOI 10.1111/j.0960-7412.2004.02059.x
  • Digby, G.J., Lober, R.M., Sethi, P.R., Lambert, N.A., Some G protein heterotrimers physically dissociate in living cells (2006) Proceedings of the National Academy of Sciences of the United States of America, 103 (47), pp. 17789-17794. , DOI 10.1073/pnas.0607116103
  • Dische, Z., General color reactions (1962) Methods in Carbohydrate Chemistry, pp. 478-481. , Whistler R.L. andWolfrom M. L., eds (New York: Academic Press)
  • Ellis, C., Turner, J.G., The Arabidopsis mutant cev1 has constitutively active jasmonate and ethylene signal pathways and enhanced resistance to pathogens (2001) Plant Cell, 13 (5), pp. 1025-1033. , DOI 10.1105/tpc.13.5.1025
  • Ferrari, S., Galletti, R., Vairo, D., Cervone, F., De Lorenzo, G., Antisense expression of the Arabidopsis thaliana AtPGIP1 gene reduces polygalacturonase-inhibiting protein accumulation and enhances susceptibility to Botrytis cinerea (2006) Molecular Plant-Microbe Interactions, 19 (8), pp. 931-936. , DOI 10.1094/MPMI-19-0931
  • Filisetti-Cozzi, T.M., Carpita, N.C., Measurement of uronic acids without interference from neutral sugars (1991) Anal. Biochem., 197, pp. 157-162
  • Glawischnig, E., Camalexin (2007) Phytochemistry, 68 (4), pp. 401-406. , DOI 10.1016/j.phytochem.2006.12.005, PII S0031942206007631
  • Grigston, J.C., Osuna, D., Scheible, W.R., Liu, C., Stitt, M., Jones, A.M., D-Glucose sensing by a plasma membrana regulator of G signaling protein, AtRGS1 (2008) FEBS Lett., 582, pp. 3577-3584
  • Hématy, K., Höfte, H., Novel receptor kinases involved in growth regulation (2008) Curr. Opin. Plant Biol., 11, pp. 321-328
  • Hernandez-Blanco, C., Feng, D.X., Hu, J., Sanchez-Vallet, A., Deslandes, L., Llorente, F., Berrocal-Lobo, M., Molinaa, A., Impairment of cellulose synthases required for Arabidopsis secondary cell wall formation enhances disease resistance (2007) Plant Cell, 19 (3), pp. 890-903. , http://www.plantcell.org/cgi/reprint/19/3/890, DOI 10.1105/tpc.106.048058
  • Huang, J., Taylor, J.P., Chen, J.-G., Uhrig, J.F., Schnell, D.J., Nakagawa, T., Korth, K.L., Jones, A.M., The plastid protein THYLAKOID FORMATION1 and the plasma membrane G-protein GPA1 interact in a novel sugar-signaling mechanism in Arabidopsis (2006) Plant Cell, 18 (5), pp. 1226-1238. , DOI 10.1105/tpc.105.037259
  • Humphrey, T.V., Bonetta, D.T., Goring, D.R., Sentinels at thewall:cellwallreceptorsandsensors (2007) NewPhytol, 176, pp. 7-21
  • Johnston, C.A., Willard, M.D., Kimple, A.J., Siderovski, D.P., Willard, F.S., A sweet cycle for Arabidopsis G-proteins: Recent discoveries and controversies in plant G-protein signal transduction (2008) Plant Signaling and Behavior, 3 (12), pp. 1067-1076. , http://www.landesbioscience.com/journals/psb/article/JohnstonPSB3-12.pdf
  • Jones, A.M., Ecker, J.R., Chen, J.-G., A reevaluation of the role of the heterotrimeric G protein in coupling light responses in arabidopsis (2003) Plant Physiology, 131 (4), pp. 1623-1627. , DOI 10.1104/pp.102.017624
  • Joo, J.H., Wang, S., Chen, J.G., Jones, A.M., Fedoroff, N.V., Different signaling and cell death roles of heterotrimeric G protein αand β subunits in the Arabidopsis oxidative stress response to ozone (2005) Plant Cell, 17 (3), pp. 957-970. , DOI 10.1105/tpc.104.029603
  • Kato, C., Mizutani, T., Tamaki, H., Kumagai, H., Kamiya, T., Hirobe, A., Fujisawa, Y., Iwasaki, Y., Characterization of heterotrimeric G protein complexes in rice plasma membrane (2004) Plant Journal, 38 (2), pp. 320-331. , DOI 10.1111/j.1365-313X.2004.02046.x
  • Kato, H., Motomura, T., Komeda, Y., Saito, T., Kato, A., Overexpression of the NAC transcription factor family gene ANAC036 results in a dwarf phenotype in Arabidopsis thaliana (2010) J. Plant Physiol., 167, pp. 571-577
  • Kauráková, M., Capek, P., Sasinková, V., Wellner, N., Ebringerová, A., FT-IR study of plant cell wall model compounds: Pectic polysaccharides and hemicelluloses (2000) Carbohydr. Polym., 43, pp. 195-203
  • Kemsley, E.K., Discriminant analysis of high-dimensional data: A comparison of principal components analysis and partial least squares data reduction methods (1996) Chemometrics and Intelligent Laboratory Systems, 33 (1), pp. 47-61. , DOI 10.1016/0169-7439(95)00090-9
  • Kliebenstein, D.J., Rowe, H.C., Denby, K.J., Secondary metabolitesinfluenceArabidopsis/Botrytis interactions: Variation inhostproductionandpathogensensitivity (2005) PlantJ., 44, pp. 25-36
  • Lease, K.A., Wen, J., Li, J., Doke, J.T., Liscum, E., Walker, J.C., A mutant arabidopsis heterotrimeric G-protein β subunit affects leaf, flower, and fruit development (2001) Plant Cell, 13 (12), pp. 2631-2641. , DOI 10.1105/tpc.13.12.2631
  • Levy, A., Erlanger, M., Rosenthal, M., Epel, B.L., A plasmodesmata-associated beta-1,3-glucanase in Arabidopsis (2007) Plant J., 49, pp. 669-682
  • Lionetti, V., Raiola, A., Camardella, L., Giovane, A., Obel, N., Pauly, M., Favaron, F., Bellincampi, D., Overexpression of pectin methylesterase inhibitors in Arabidopsis restricts fungal infection by Botrytis cinerea (2007) Plant Physiology, 143 (4), pp. 1871-1880. , DOI 10.1104/pp.106.090803
  • Llorente, F., Alonso-Blanco, C., Sanchez-Rodriguez, C., Jorda, L., Molina, A., ERECTA receptor-like kinase and heterotrimeric G protein from Arabidopsis are required for resistance to the necrotrophic fungus Plectosphaerella cucumerina (2005) Plant Journal, 43 (2), pp. 165-180. , DOI 10.1111/j.1365-313X.2005.02440.x
  • Llorente, F., Muskett, P., Sánchez-Vallet, A., Lopez, G., Ramos, B., Sánchez-Rodriguez, C., Jordá, L., Molina, A., Repression of the auxin response pathway increases Arabidopsis susceptibility to necrotrophic fungi (2008) Mol. Plant., (1), pp. 496-509
  • Maeda, K., Houjyou, Y., Komatsu, T., Hori, H., Kodaira, T., Ishikawa, A., AGB1 and PMR5 contribute to PEN2- mediated preinvasion resistance to Magnaporthe oryzae in Arabidopsis thaliana.Mol (2009) Plant-Microbe Interact., 22, pp. 1331-1340
  • Marrari, Y., Crouthamel, M., Irannejad, R., Wedegaertner, P.B., Assembly and trafficking of heterotrimeric G proteins (2007) Biochemistry, 46 (26), pp. 7665-7677. , DOI 10.1021/bi700338m
  • Mason, M.G., Botella, J.R., Isolation of a novel G-protein γ-subunit from Arabidopsis thaliana and its interaction with Gβ (2001) Biochimica et Biophysica Acta - Gene Structure and Expression, 1520 (2), pp. 147-153. , DOI 10.1016/S0167-4781(01)00262-7, PII S0167478101002627
  • Miedes, E., Lorences, E.P., The implication of xyloglucan endotransglucosylase/hydrolase (XTHs) in tomato fruit infection by Penicillium expansum Link. A (2007) Journal of Agricultural and Food Chemistry, 55 (22), pp. 9021-9026. , DOI 10.1021/jf0718244
  • Mutwil, M., Usadle, B., Schütte, M., Loraine, A., Ebenhöh, O., Persson, S., Assembly of an interactive correlation network for the Arabidopsis genome using a novel heuristic clustering algorithm (2010) Plant Physiol., (152), pp. 29-43
  • Neumetzler, L., (2010) Identification and Characterization of Arabidopsis Mutants Associated with Xyloglucan Metabolism, pp. 33-35. , (Berlin: Rhombos-Verlag)
  • Nishitani, K., Tominaga, R., Endo-xyloglucantransferase, a novel class of glycosyl transferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule (1992) J. Biol. Chem., 267, pp. 21058-21064
  • Pandey, S., Chen, J.-G., Jones, A.M., Assmann, S.M., G-protein complex mutants are hypersensitive to abscisic acid regulation of germination and postgermination development (2006) Plant Physiology, 141 (1), pp. 243-256. , DOI 10.1104/pp.106.079038
  • Persson, S., Wei, H., Milne, J., Page, G.P., Somerville, C.R., Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets (2005) Proceedings of the National Academy of Sciences of the United States of America, 102 (24), pp. 8633-8638. , DOI 10.1073/pnas.0503392102
  • Pfalz, M., Mikkelsen, M.D., Bednarek, P., Olsen, C.E., Halkier, B.A., Kroymann, J., Metabolic engineering in Nicotiana benthamiana reveals key enzyme functions in Arabidopsis indole glucosinolate modification (2011) Plant Cell., 23, pp. 716-729
  • Pfalz, M., Vogel, H., Kroymann, J., The gene controlling the indole glucosinolate modifier1 quantitative trait locus alters indole glucosinolate structures and aphid resistance in Arabidopsis (2009) Plant Cell., 21, pp. 985-999
  • Philip, B., Levin, D.E., Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1 (2001) Mol. Cell Biol., 21, pp. 271-280
  • Raiola, A., Lionetti, V., Elmaghraby, I., Immerzeel, P., Mellerowicz, E.J., Salvi, G., Cervone, F., Bellincampi, D., Pectin methylesterase is induced in Arabidopsis upon infection and is necessary for a successful colonization by necrotrophic pathogens (2010) Mol. Plant-Microbe Interact., (24), pp. 432-440
  • Ramírez, V., Agorio, A., Coego, A., García-Andrade, J., Hernández, M.J., Balaguer, B., Ouwerkerk, P.B., Vera, P., MYB46 modulates disease susceptibility to Botrytis cinerea in Arabidopsis (2011) Plant Physiol., 155, pp. 1920-1935
  • Reiter, W.-D., Chapple, C.C.S., Somerville, C.R., Altered growth and cell walls in a fucose-deficient mutant of Arabidopsis (1993) Science, 261 (5124), pp. 1032-1035
  • Rieu, I., Powers, S.J., Real-time quantitative RT-PCR: Design, calculations, and statistics (2009) Plant Cell., 21, pp. 1031-1033
  • Rogers, L.A., Dubos, C., Surman, C., Willment, J., Cullis, I.F., Mansfield, S.D., Campbell, M.M., Comparison of lignin deposition in three ectopic lignification mutants (2005) New Phytologist., 168, pp. 123-140
  • Sampedro, J., Pardo, B., Gianzo, C., Guitian, E., Revilla, G., Zarra, I., Lack of alpha-xylosidase activity in Arabidopsis alters xyloglucan composition and results in growth defects (2010) Plant Physiol., 154, pp. 1105-1115
  • Sánchez-Rodriguez, C., Estevez, J.M., Llorente, F., Hernandez-Blanco, C., Jordá, L., Pagan, I., Berrocal, M., Molina, A., The ERECTA receptorlike kinase regulates cell wall mediated resistance to pathogens in Arabidopsis thaliana (2009) Mol. Plant-Microbe Interact., 22, pp. 953-963
  • Sánchez-Vallet, A., Ramos, B., Bednarek, P., López, G., Piślewska-Bednarek, M., Schulze-Lefert, P., Molina, A., Tryptophanderived secondary metabolites in Arabidopsis thaliana confer non-host resistance to necrotrophic Plectosphaerella cucumerina fungi (2010) Plant J., 63, pp. 115-127
  • Schuhegger, R., Nafisi, M., Mansourova, M., Petersen, B.L., Olsen, C.E., Svatos, A., Halkier, B.A., Glawischnig, E., CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis (2006) Plant Physiology, 141 (4), pp. 1248-1254. , DOI 10.1104/pp.106.082024
  • Selvendran, R., March, J., Ring, S., Determination of aldoses and uronic acid content of vegetable fiber (1979) Anal. Biochem., 96, pp. 282-292
  • Somerville, C., Bauer, S., Brininstool, G., Facette, M., Hamann, T., Milne, J., Osborne, E., Youngs, H., Toward a systems approach to understanding plant cell walls (2004) Science, 306 (5705), pp. 2206-2211. , DOI 10.1126/science.1102765
  • Stein, M., Dittgen, J., Sanchez-Rodriguez, C., Hou, B.-H., Molina, A., Schulze-Lefert, P., Lipka, V., Somerville, S., Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration (2006) Plant Cell, 18 (3), pp. 731-746. , DOI 10.1105/tpc.105.038372
  • Stevenson, T.T., McNeil, M., Darvill, A.G., Albersheim, P., Structure of plant cell walls. XVIIT. An analysis of the extracellular polysaccharides of suspension-cultured sycamore cells (1986) Plant Physiol., 80, pp. 1012-1019
  • Suharsono, U., Fujisawa, Y., Kawasaki, T., Iwasaki, Y., Satoh, H., Shimamoto, K., The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice (2002) Proc. Natl Acad. Sci. U S A., 99, pp. 13307-13312
  • Temple, B.R.S., Jones, A.M., The plant heterotrimeric G-protein complex (2007) Annual Review of Plant Biology, 58, pp. 249-266. , DOI 10.1146/annurev.arplant.58.032806.103827
  • Thomma, B.P.H.J., Eggermont, K., Penninckx, I.A.M.A., Mauch-Mani, B., Vogelsang, R., Cammue, B.P.A., Broekaert, W.F., Separate jasmonate-dependent and salicylate-dependent defense-response pathways in arabidopsis are essential for resistance to distinct microbial pathogens (1998) Proceedings of the National Academy of Sciences of the United States of America, 95 (25), pp. 15107-15111. , DOI 10.1073/pnas.95.25.15107
  • Trusov, Y., Jordá, L., Molina, A., Botella, J.R., G proteins and plant innate immunity (2010) Integrated G Proteins Signaling in Plants, pp. 221-250. , Yalovsky, S., Balus?ka, F. and Jones, A., eds ( : Signaling and Communication in Plants Series Springer, Berlin)
  • Trusov, Y., Rookes, J.E., Chakravorty, D., Armour, D., Schenk, P.M., Botella, J.R., Heterotrimeric G proteins facilitate arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling (2006) Plant Physiology, 140 (1), pp. 210-220. , DOI 10.1104/pp.105.069625
  • Trusov, Y., Rookes, J.E., Tilbrook, K., Chakravorty, D., Mason, M.G., Anderson, D., Chen, J.-G., Botella, J.R., Heterotrimeric G protein γ subunits provide functional selectivity in Gβγ dimer signaling in Arabidopsis (2007) Plant Cell, 19 (4), pp. 1235-1250. , http://www.plantcell.org/cgi/reprint/19/4/1235, DOI 10.1105/tpc.107.050096
  • Trusov, Y., Sewelam, N., Rookes, J.E., Kunkel, M., Nowak, E., Schenk, P.M., Botella, J.R., Heterotrimeric G proteins-mediated resistance to necrotrophic pathogens includes mechanisms independent of salicylic acid-, jasmonic acid/ethylene- and abscisic acid-mediated defense signaling (2008) Plant J., 58, pp. 69-81
  • Trusov, Y., Zhang, W., Assmann, S.M., Botella, J.R., Gγ1 + Gγ2 ≠ Gβ: Heterotrimeric G protein gγ-deficient mutants do not recapitulate all phenotypes of Gβ-deficient mutants (2008) Plant Physiology, 147 (2), pp. 636-649. , http://www.plantphysiol.org/cgi/reprint/147/2/636, DOI 10.1104/pp.108.117655
  • Ullah, H., Chen, J.-G., Temple, B., Boyes, D.C., Alonso, J.M., Davis, K.R., Ecker, J.R., Jones, A.M., The β-subunit of the arabidopsis G protein negatively regulates auxin-induced cell division and affects multiple developmental processes (2003) Plant Cell, 15 (2), pp. 393-409. , DOI 10.1105/tpc.006148
  • Ullah, H., Chen, J.-G., Wang, S., Jones, A.M., Role of a heterotrimeric G protein in regulation of Arabidopsis seed germination (2002) Plant Physiology, 129 (2), pp. 897-907. , DOI 10.1104/pp.005017
  • Van Kan, J.A.L., Licensed to kill: The lifestyle of a necrotrophic plant pathogen (2006) Trends Plant Sci., 11, pp. 247-253
  • Vilim, V., Colorimetric estimation of uronic acids using 2-hydroxydiphenyl as a reagent (1985) Biomedica Biochimica Acta, 44 (11-12), pp. 1717-1720
  • Vogel, J.P., Raab, T.K., Schiff, C., Somerville, S.C., PMR6, a pectate lyase-like gene required for powdery mildew susceptibility in Arabidopsis (2002) Plant Cell., 14, pp. 2095-2106
  • Vogel, J.P., Raab, T.K., Somerville, C.R., Somerville, S.C., Mutations in PMR5 result in powdery mildew resistance and altered cell wall composition (2004) Plant Journal, 40 (6), pp. 968-978. , DOI 10.1111/j.1365-313X.2004.02264.x
  • Wang, H.X., Weerasinghe, R.R., Perdue, T.D., Cakmakci, N.G., Taylor, J.P., Marzluff, W.F., Jones, A.M., A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis (2006) Molecular Biology of the Cell, 17 (10), pp. 4257-4269. , DOI 10.1091/mbc.E06-01-0046
  • Wang, X.-Q., Ullah, H., Jones, A.M., Assmann, S.M., G protein regulation of ion channels and abscisic acid signaling in Arabidopsis guard cells (2001) Science, 292 (5524), pp. 2070-2072. , DOI 10.1126/science.1059046
  • Wilson, R.H., Smith, A.C., Kauráková, M., Saunders, P., Wellner, N., Waldron, K.W., The mechanical properties and molecular dynamics of plant cell wall polysaccharides studied by Fourier Infrared Spectroscopy (2000) Plant Physiol., 124, pp. 397-405
  • Zhang, Y., Devries, M.E., Skolnick, J., Structure modeling of all identified G protein-coupled receptors in the human genome (2006) PLoS Comput. Biol., 2, pp. e13
  • Zhu, H., Li, G.J., Ding, L., Cui, X., Berg, H., Assmann, S.M., Xia, Y., Arabidopsis extra large G-protein 2 (XLG2) interacts with the Gbeta subunit of heterotrimeric G protein and functions in disease resistance (2009) Mol. Plant., 2, pp. 513-525

Citas:

---------- APA ----------
Delgado-Cerezo, M., Sánchez-Rodríguez, C., Escudero, V., Miedes, E., Fernández, P.V., Jordá, L., Hernández-Blanco, C.,..., Molina, A. (2012) . Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi. Molecular Plant, 5(1), 98-114.
http://dx.doi.org/10.1093/mp/ssr082
---------- CHICAGO ----------
Delgado-Cerezo, M., Sánchez-Rodríguez, C., Escudero, V., Miedes, E., Fernández, P.V., Jordá, L., et al. "Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi" . Molecular Plant 5, no. 1 (2012) : 98-114.
http://dx.doi.org/10.1093/mp/ssr082
---------- MLA ----------
Delgado-Cerezo, M., Sánchez-Rodríguez, C., Escudero, V., Miedes, E., Fernández, P.V., Jordá, L., et al. "Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi" . Molecular Plant, vol. 5, no. 1, 2012, pp. 98-114.
http://dx.doi.org/10.1093/mp/ssr082
---------- VANCOUVER ----------
Delgado-Cerezo, M., Sánchez-Rodríguez, C., Escudero, V., Miedes, E., Fernández, P.V., Jordá, L., et al. Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi. Mol. Plant. 2012;5(1):98-114.
http://dx.doi.org/10.1093/mp/ssr082