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Abstract:

We describe neurobiological applications of RuBi-Glutamate, a novel caged-glutamate compound based on ruthenium photochemistry. RuBi-Glutamate can be excited with visible wavelengths and releases glutamate after one- or two-photon excitation. It has high quantum efficiency and can be used at low concentrations, partly avoiding the blockade of GABAergic transmission present with other caged compounds. Two-photon uncaging of RuBi-Glutamate has a high spatial resolution and generates excitatory responses in individual dendritic spines with physiological kinetics. With laser beam multiplexing, two-photon RuBi-Glutamate uncaging can also be used to depolarize and fire pyramidal neurons with single-cell resolution. RuBi-Glutamate therefore enables the photoactivation of neuronal dendrites and circuits with visible or two-photon light sources, achieving single cell, or even single spine, precision. © 2009 Fino, Araya, Peterka, Salierno, Etchenique and Yuste.

Registro:

Documento: Artículo
Título:RuBi-Glutamate: Two-photon and visible-light photoactivation of neurons and dendritic spines
Autor:Fino, E.; Araya, R.; Peterka, D.S.; Salierno, M.; Etchenique, R.; Yuste, R.
Filiación:Howard Hughes Medical Institute, Department of Biological Sciences, Columbia University, New York, NY, United States
Departamento de Química Inorgánica Analítica y Química Física, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Bipyridine; RuBi-GABA; Ruthenium; Uncaging
Año:2009
Volumen:3
Número:MAY
DOI: http://dx.doi.org/10.3389/neuro.04.002.2009
Título revista:Frontiers in Neural Circuits
Título revista abreviado:Front. Neural Circuits
ISSN:16625110
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16625110_v3_nMAY_p_Fino

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Citas:

---------- APA ----------
Fino, E., Araya, R., Peterka, D.S., Salierno, M., Etchenique, R. & Yuste, R. (2009) . RuBi-Glutamate: Two-photon and visible-light photoactivation of neurons and dendritic spines. Frontiers in Neural Circuits, 3(MAY).
http://dx.doi.org/10.3389/neuro.04.002.2009
---------- CHICAGO ----------
Fino, E., Araya, R., Peterka, D.S., Salierno, M., Etchenique, R., Yuste, R. "RuBi-Glutamate: Two-photon and visible-light photoactivation of neurons and dendritic spines" . Frontiers in Neural Circuits 3, no. MAY (2009).
http://dx.doi.org/10.3389/neuro.04.002.2009
---------- MLA ----------
Fino, E., Araya, R., Peterka, D.S., Salierno, M., Etchenique, R., Yuste, R. "RuBi-Glutamate: Two-photon and visible-light photoactivation of neurons and dendritic spines" . Frontiers in Neural Circuits, vol. 3, no. MAY, 2009.
http://dx.doi.org/10.3389/neuro.04.002.2009
---------- VANCOUVER ----------
Fino, E., Araya, R., Peterka, D.S., Salierno, M., Etchenique, R., Yuste, R. RuBi-Glutamate: Two-photon and visible-light photoactivation of neurons and dendritic spines. Front. Neural Circuits. 2009;3(MAY).
http://dx.doi.org/10.3389/neuro.04.002.2009