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Abstract:

We describe the selective photorelease of γ-amino butyric acid (GABA) with a novel caged-GABA compound that uses a ruthenium complex as photosensor. This compound ("RuBi-GABA") can be excited with visible wavelengths, providing greater tissue penetration, less photo-toxicity, and faster photorelease kinetics than currently used UV light-sensitive caged compounds. Using pyramidal neurons from neocortical brain slices, we show that RuBi-GABA uncaging induces GABA-A receptor-mediated responses, has no detectable side effects on endogenous GABAergic and glutamatergic receptors and generates responses with kinetics and spatial resolution comparable to the best caged GABA compounds presently available. Finally, we illustrate two potential applications of RuBi-GABA uncaging: GABA receptor mapping, and optical silencing of neuronal firing. © 2008 Rial Verde, Zayat, Etchenique and Yuste.

Registro:

Documento: Artículo
Título:Photorelease of GABA with visible light using an inorganic caging group
Autor:Verde, E.M.R.; Zayat, L.; Etchenique, R.; Yuste, R.
Filiación:Department of Biological Sciences, Howard Hughes Medical Institute, Columbia University, New York, NY, United States
Departamento de Química Inorgánica, Analítica y Química Física - INQUIMAE, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:GABA; Inhibition; Photorelease; Ruthenium; Uncaging
Año:2008
Volumen:2
Número:AUG
DOI: http://dx.doi.org/10.3389/neuro.04.002.2008
Título revista:Frontiers in Neural Circuits
Título revista abreviado:Front. Neural Circuits
ISSN:16625110
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16625110_v2_nAUG_p_Verde

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Citas:

---------- APA ----------
Verde, E.M.R., Zayat, L., Etchenique, R. & Yuste, R. (2008) . Photorelease of GABA with visible light using an inorganic caging group. Frontiers in Neural Circuits, 2(AUG).
http://dx.doi.org/10.3389/neuro.04.002.2008
---------- CHICAGO ----------
Verde, E.M.R., Zayat, L., Etchenique, R., Yuste, R. "Photorelease of GABA with visible light using an inorganic caging group" . Frontiers in Neural Circuits 2, no. AUG (2008).
http://dx.doi.org/10.3389/neuro.04.002.2008
---------- MLA ----------
Verde, E.M.R., Zayat, L., Etchenique, R., Yuste, R. "Photorelease of GABA with visible light using an inorganic caging group" . Frontiers in Neural Circuits, vol. 2, no. AUG, 2008.
http://dx.doi.org/10.3389/neuro.04.002.2008
---------- VANCOUVER ----------
Verde, E.M.R., Zayat, L., Etchenique, R., Yuste, R. Photorelease of GABA with visible light using an inorganic caging group. Front. Neural Circuits. 2008;2(AUG).
http://dx.doi.org/10.3389/neuro.04.002.2008