Artículo

Moya-Díaz, J.; Álvarez, Y.D.; Montenegro, M.; Bayonés, L.; Belingheri, A.V.; González-Jamett, A.M.; Cárdenas, A.M.; Marengo, F.D. "Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process" (2016) Frontiers in Cellular Neuroscience. 10(JUL)
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Abstract:

Under basal conditions the action potential firing rate of adrenal chromaffin cells is lower than 0.5 Hz. The maintenance of the secretory response at such frequencies requires a continuous replenishment of releasable vesicles. However, the mechanism that allows such vesicle replenishment remains unclear. Here, using membrane capacitance measurements on mouse chromaffin cells, we studied the mechanism of replenishment of a group of vesicles released by a single action potential-like stimulus (APls). The exocytosis triggered by APls (ETAP) represents a fraction (40%) of the immediately releasable pool, a group of vesicles highly coupled to voltage dependent calcium channels. ETAP was replenished with a time constant of 0.73 ± 0.11 s, fast enough to maintain synchronous exocytosis at 0.2-0.5 Hz stimulation. Regarding the mechanism involved in rapid ETAP replenishment, we found that it depends on the ready releasable pool; indeed depletion of this vesicle pool significantly delays ETAP replenishment. On the other hand, ETAP replenishment also correlates with a dynamin-dependent fast endocytosis process (τ = 0.53 ± 0.01 s). In this regard, disruption of dynamin function markedly inhibits the fast endocytosis and delays ETAP replenishment, but also significantly decreases the synchronous exocytosis during repetitive APls stimulation at low frequencies (0.2 and 0.5 Hz). Considering these findings, we propose a model in where both the transfer of vesicles from ready releasable pool and fast endocytosis allow rapid ETAP replenishment during low stimulation frequencies. © 2016 Moya-Díaz, Álvarez, Montenegro, Bayonés, Belingheri, González- Jamett, Cárdenas and Marengo.

Registro:

Documento: Artículo
Título:Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process
Autor:Moya-Díaz, J.; Álvarez, Y.D.; Montenegro, M.; Bayonés, L.; Belingheri, A.V.; González-Jamett, A.M.; Cárdenas, A.M.; Marengo, F.D.
Filiación:Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Instituto de Fisiología, Biología Molecular y Neurociencias, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso, Valparaíso, Chile
Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Ca2+ current; Dynamin; Endocytosis; Immediately releasable pool; Membrane capacitance; Secretion; amphiphysin; calcium ion; dynamin; hybrid protein; voltage gated calcium channel; Wnt2 protein; action potential; animal cell; animal experiment; animal model; animal tissue; Article; blood vessel capacitance; calcium current; chromaffin cell; controlled study; depolarization; electrostimulation; endocytosis; exocytosis; female; firing rate; low frequency noise; male; mouse; nonhuman; Purkinje fiber; signal processing; transport vesicle; whole cell patch clamp
Año:2016
Volumen:10
Número:JUL
DOI: http://dx.doi.org/10.3389/fncel.2016.00184
Título revista:Frontiers in Cellular Neuroscience
Título revista abreviado:Front. Cell. Neurosci.
ISSN:16625102
CAS:amphiphysin, 158624-13-6; calcium ion, 14127-61-8; dynamin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16625102_v10_nJUL_p_MoyaDiaz

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Citas:

---------- APA ----------
Moya-Díaz, J., Álvarez, Y.D., Montenegro, M., Bayonés, L., Belingheri, A.V., González-Jamett, A.M., Cárdenas, A.M.,..., Marengo, F.D. (2016) . Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process. Frontiers in Cellular Neuroscience, 10(JUL).
http://dx.doi.org/10.3389/fncel.2016.00184
---------- CHICAGO ----------
Moya-Díaz, J., Álvarez, Y.D., Montenegro, M., Bayonés, L., Belingheri, A.V., González-Jamett, A.M., et al. "Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process" . Frontiers in Cellular Neuroscience 10, no. JUL (2016).
http://dx.doi.org/10.3389/fncel.2016.00184
---------- MLA ----------
Moya-Díaz, J., Álvarez, Y.D., Montenegro, M., Bayonés, L., Belingheri, A.V., González-Jamett, A.M., et al. "Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process" . Frontiers in Cellular Neuroscience, vol. 10, no. JUL, 2016.
http://dx.doi.org/10.3389/fncel.2016.00184
---------- VANCOUVER ----------
Moya-Díaz, J., Álvarez, Y.D., Montenegro, M., Bayonés, L., Belingheri, A.V., González-Jamett, A.M., et al. Sustained exocytosis after action potential-like stimulation at low frequencies in mouse chromaffin cells depends on a dynamin-dependent fast endocytotic process. Front. Cell. Neurosci. 2016;10(JUL).
http://dx.doi.org/10.3389/fncel.2016.00184