Artículo

Marginedas-Freixa, I.; Alvarez, C.L.; Moras, M.; Hattab, C.; Bouyer, G.; Chene, A.; Lefevre, S.D.; Le Van Kim, C.; Bihel, F.; Schwarzbaum, P.J.; Ostuni, M.A. "Induction of ATP release, PPIX transport, and cholesterol uptake by human red blood cells using a new family of TSPO ligands" (2018) International Journal of Molecular Sciences. 19(10)
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Abstract:

Two main isoforms of the Translocator Protein (TSPO) have been identified. TSPO1 is ubiquitous and is mainly present at the outer mitochondrial membrane of most eukaryotic cells, whereas, TSPO2 is specific to the erythroid lineage, located at the plasma membrane, the nucleus, and the endoplasmic reticulum. The design of specific tools is necessary to determine the molecular associations and functions of TSPO, which remain controversial nowadays. We recently demonstrated that TSPO2 is involved in a supramolecular complex of the erythrocyte membrane, where micromolar doses of the classical TSPO ligands induce ATP release and zinc protoporphyrin (ZnPPIX) transport. In this work, three newly-designed ligands (NCS1016, NCS1018, and NCS1026) were assessed for their ability to modulate the functions of various erythrocyte’s and compare them to the TSPO classical ligands. The three new ligands were effective in reducing intraerythrocytic Plasmodium growth, without compromising erythrocyte survival. While NCS1016 and NCS1018 were the most effective ligands in delaying sorbitol-induced hemolysis, NCS1016 induced the highest uptake of ZnPPIX and NCS1026 was the only ligand inhibiting the cholesterol uptake. Differential effects of ligands are probably due, not only, to ligand features, but also to the dynamic interaction of TSPO with various partners at the cell membrane. Further studies are necessary to fully understand the mechanisms of the TSPO’s complex activation. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.

Registro:

Documento: Artículo
Título:Induction of ATP release, PPIX transport, and cholesterol uptake by human red blood cells using a new family of TSPO ligands
Autor:Marginedas-Freixa, I.; Alvarez, C.L.; Moras, M.; Hattab, C.; Bouyer, G.; Chene, A.; Lefevre, S.D.; Le Van Kim, C.; Bihel, F.; Schwarzbaum, P.J.; Ostuni, M.A.
Filiación:UMR-S1134, Integrated Biology of Red Blood Cells, INSERM, Université Paris Diderot, Sorbonne Paris Cité, Université de la Réunion, Université des Antilles, Paris, F-75015, France
Institut National de la Transfusion Sanguine, Laboratoire d’Excellence GR-Ex, Paris, F-75015, France
Instituto de Química y Fisico-Química Biológicas “Prof. Alejandro C. Paladini”, UBA, CONICET, Facultad de Farmacia y Bioquímica, Junín 956, Buenos Aires, C1113AAD, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, C1113AAD, Argentina
UMR 8227 LBI2M, Comparative Erythrocyte’s Physiology, CNRS, Sorbonne Université, Laboratoire d’Excellence GR-Ex, Roscoff, F-29680, France
UMR7200, Laboratoire d’Innovation Thérapeutique, Faculty of Pharmacy, University of Strasbourg, CNRS, Illkirch Graffenstaden, F-67400, France
Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, C1113AAD, Argentina
Palabras clave:ATP; Erythrocyte; Malaria; Plasmodium; Red blood cell; TSPO; TSPO2; VDAC; ZnPPIX; adenine nucleotide translocase; adenosine triphosphate; antiparasitic agent; carrier protein; ligand; n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide; n,n diethyl 2 (2 (furan 2 yl) 5 oxoimidazo[1 c]quinazolin 6(5h) yl)acetamide; n,n diethyl 2 (2 (furan 2 yl) 5 oxomidazo[1 c]quinazolin 6(5h) yl)acetamide; n,n dipropyl 2 (5 oxo 2 phenylimidazo[1 c]quinazolin 6(5h) yl)acetamide; protoporphyrin zinc; radioligand; reactive oxygen metabolite; Ro5 4864; sorbitol; SSR 180 575; ubiquitin; unclassified drug; voltage dependent anion channel; 4 aminobutyric acid receptor; adenosine triphosphate; cholesterol; ligand; protein binding; protoporphyrin; reactive oxygen metabolite; sorbitol; TSPO protein, human; antimalarial activity; Article; binding affinity; blood flow; cholesterol transport; comparative study; controlled study; erythrocyte; erythrocyte function and characteristics; erythrocyte lifespan; erythrocyte membrane; flow cytometry; fluorescence; growth inhibition; hematocrit; hemolysis assay; human; human cell; IC50; malaria falciparum; normal human; parasitemia; plasmodium (life cycle stage); protein analysis; protein transport; supramolecular chemistry; tissue oxygenation; drug effect; hemolysis; metabolism; Plasmodium falciparum; transport at the cellular level; Adenosine Triphosphate; Biological Transport; Cholesterol; Erythrocytes; Hemolysis; Humans; Ligands; Plasmodium falciparum; Protein Binding; Protoporphyrins; Reactive Oxygen Species; Receptors, GABA; Sorbitol
Año:2018
Volumen:19
Número:10
DOI: http://dx.doi.org/10.3390/ijms19103098
Título revista:International Journal of Molecular Sciences
Título revista abreviado:Int. J. Mol. Sci.
ISSN:16616596
CAS:adenine nucleotide translocase, 9068-80-8; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; carrier protein, 80700-39-6; n sec butyl 1 (2 chlorophenyl) n methyl 3 isoquinolinecarboxamide, 85532-75-8; protoporphyrin zinc, 15442-64-5; sorbitol, 26566-34-7, 50-70-4, 53469-19-5; ubiquitin, 60267-61-0; cholesterol, 57-88-5; protoporphyrin, 553-12-8; Adenosine Triphosphate; Cholesterol; Ligands; protoporphyrin IX; Protoporphyrins; Reactive Oxygen Species; Receptors, GABA; Sorbitol; TSPO protein, human
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16616596_v19_n10_p_MarginedasFreixa

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Citas:

---------- APA ----------
Marginedas-Freixa, I., Alvarez, C.L., Moras, M., Hattab, C., Bouyer, G., Chene, A., Lefevre, S.D.,..., Ostuni, M.A. (2018) . Induction of ATP release, PPIX transport, and cholesterol uptake by human red blood cells using a new family of TSPO ligands. International Journal of Molecular Sciences, 19(10).
http://dx.doi.org/10.3390/ijms19103098
---------- CHICAGO ----------
Marginedas-Freixa, I., Alvarez, C.L., Moras, M., Hattab, C., Bouyer, G., Chene, A., et al. "Induction of ATP release, PPIX transport, and cholesterol uptake by human red blood cells using a new family of TSPO ligands" . International Journal of Molecular Sciences 19, no. 10 (2018).
http://dx.doi.org/10.3390/ijms19103098
---------- MLA ----------
Marginedas-Freixa, I., Alvarez, C.L., Moras, M., Hattab, C., Bouyer, G., Chene, A., et al. "Induction of ATP release, PPIX transport, and cholesterol uptake by human red blood cells using a new family of TSPO ligands" . International Journal of Molecular Sciences, vol. 19, no. 10, 2018.
http://dx.doi.org/10.3390/ijms19103098
---------- VANCOUVER ----------
Marginedas-Freixa, I., Alvarez, C.L., Moras, M., Hattab, C., Bouyer, G., Chene, A., et al. Induction of ATP release, PPIX transport, and cholesterol uptake by human red blood cells using a new family of TSPO ligands. Int. J. Mol. Sci. 2018;19(10).
http://dx.doi.org/10.3390/ijms19103098