Artículo

Fazio, A.T.; Bertoni, M.D.; Adler, M.T.; Ruiz, L.B.; Rosso, M.L.; Muggia, L.; Hager, A.; Stocker-Wörgötter, E.; Maier, M.S. "Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions" (2009) Mycological Progress. 8(4):359-365
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Abstract:

A strain of the lichen mycobiont isolated from a thallus of Parmotrema reticulatum was cultured axenically on different media. The morphology, anatomy, growth of the colonies, and metabolite production were studied. The isolated fungal colonies developed well and showed a remarkable morphogenetic capacity on most of the assayed solid media, e. g., malt extract 2%-yeast extract 0.2% (MEYE), malt extract 1%-yeast extract 0.4%-sucrose 10% (MY10), and the original Lilly & Barnett medium (LB). The identity of the isolated fungus was confirmed by its ITS rDNA-sequence. Atranorin, the major cortical lichen depside, was produced when the colonies were grown over 5 and 10 months on solid LB medium, combined with a dessication treatment. Atranorin was identified by matching of UV spectra obtained from HPLC running and a reference substance in a spectrum library. Colonies grown on MEYE and MY10 with a dessication treatment did not produce any lichen secondary metabolite. Mycobionts grown for 5 months on solid MEYE without a dessication treatment produced triacylglycerides as the major metabolites, and the fatty acids were characterized as their methyl esters. Analysis by TLC and HPLC-DAD of extracts of colonies grown on LB and MY10 without dessication revealed that the typical secondary compounds of the natural lichen were not produced. The major metabolites of the natural lichen thallus were identified by chromatographic and spectroscopic methods. © German Mycological Society and Springer 2009.

Registro:

Documento: Artículo
Título:Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions
Autor:Fazio, A.T.; Bertoni, M.D.; Adler, M.T.; Ruiz, L.B.; Rosso, M.L.; Muggia, L.; Hager, A.; Stocker-Wörgötter, E.; Maier, M.S.
Filiación:UMYMFOR-Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina
PROPLAME-PRHIDEB-Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, 1428 Ciudad Autónoma de Buenos Aires, Argentina
Institut für Pflanzenwissenschaften, Karl Franzens University of Graz, Holteigasse 6, 8010 Graz, Austria
Department of Organismic Biology, University of Salzburg, Hellbrunner Strasse 34, 5020 Salzburg, Austria
Palabras clave:Atranorin; Lichen metabolites; Morphogenetic potential; Parmeliaceae; Triacylglycerides; Fungi; Parmeliaceae; Parmotrema reticulatum
Año:2009
Volumen:8
Número:4
Página de inicio:359
Página de fin:365
DOI: http://dx.doi.org/10.1007/s11557-009-0609-1
Título revista:Mycological Progress
Título revista abreviado:Mycol. Prog.
ISSN:1617416X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v8_n4_p359_Fazio

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Citas:

---------- APA ----------
Fazio, A.T., Bertoni, M.D., Adler, M.T., Ruiz, L.B., Rosso, M.L., Muggia, L., Hager, A.,..., Maier, M.S. (2009) . Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions. Mycological Progress, 8(4), 359-365.
http://dx.doi.org/10.1007/s11557-009-0609-1
---------- CHICAGO ----------
Fazio, A.T., Bertoni, M.D., Adler, M.T., Ruiz, L.B., Rosso, M.L., Muggia, L., et al. "Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions" . Mycological Progress 8, no. 4 (2009) : 359-365.
http://dx.doi.org/10.1007/s11557-009-0609-1
---------- MLA ----------
Fazio, A.T., Bertoni, M.D., Adler, M.T., Ruiz, L.B., Rosso, M.L., Muggia, L., et al. "Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions" . Mycological Progress, vol. 8, no. 4, 2009, pp. 359-365.
http://dx.doi.org/10.1007/s11557-009-0609-1
---------- VANCOUVER ----------
Fazio, A.T., Bertoni, M.D., Adler, M.T., Ruiz, L.B., Rosso, M.L., Muggia, L., et al. Culture studies on the mycobiont isolated from Parmotrema reticulatum (Taylor) Choisy: Metabolite production under different conditions. Mycol. Prog. 2009;8(4):359-365.
http://dx.doi.org/10.1007/s11557-009-0609-1