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Abstract:

We report the production of the perylenequinone pigment elsinochrome A in aposymbiotic culture of the mycobiont of the crustose epiphytic lichen Graphis elongata Zenker (Lecanoromycetes), collected in Argentina (Buenos Aires). The substance was not detected in the lichenized thallus (using HPLC techniques) and is otherwise only known from one unrelated lichen and a few genera of non-lichenized, plant-pathogenic fungi in the class Dothideomycetes. The phylogenetic affinities of the lichen mycobiont and the cultured fungus were confirmed using DNA sequence data of the mitochondrial small subunit rDNA (mtSSU), which place the lichen fungus into the Allographa clade within Graphidaceae. The mycobiont pigment was purified and characterized by spectroscopic methods. This is the first case where a rare pigment, otherwise known from non-lichenized, plant-pathogenic fungi, is produced in aposymbiotic culture of a lichen mycobiont, while, at the same time, being absent from the lichen thallus itself. Based on this finding, we discuss the previously postulated hypothesis that lichen mycobionts maintain secondary metabolic pathways of non-lichenized ancestors in their genome, while gene expression and production of metabolites is suppressed in the lichenized state due to toxicity to the photobiont. © 2018, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature.

Registro:

Documento: Artículo
Título:Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata
Autor:Fazio, A.T.; Adler, M.T.; Parnmen, S.; Lücking, R.; Maier, M.S.
Filiación:InMiBo (ex PROPLAME-PRHIDEB) UBA-CONICET—Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
UMYMFOR—Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón II, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EGA, Argentina
Toxicology Center, National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthabur, 11000, Thailand
Science & Education, The Field Museum, 1400 South Lake Shore, Chicago, IL 60605, United States
Botanic Garden and Botanical Museum, Freie Universität Berlin, Königin-Luise-Straße 6–8, Berlin, 14195, Germany
Palabras clave:Axenic culture; Elsinochrome A; Graphis elongata; Lichen; Perylenequinones
Año:2018
Volumen:17
Número:4
Página de inicio:479
Página de fin:487
DOI: http://dx.doi.org/10.1007/s11557-017-1374-1
Título revista:Mycological Progress
Título revista abreviado:Mycol. Prog.
ISSN:1617416X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v17_n4_p479_Fazio

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Citas:

---------- APA ----------
Fazio, A.T., Adler, M.T., Parnmen, S., Lücking, R. & Maier, M.S. (2018) . Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata. Mycological Progress, 17(4), 479-487.
http://dx.doi.org/10.1007/s11557-017-1374-1
---------- CHICAGO ----------
Fazio, A.T., Adler, M.T., Parnmen, S., Lücking, R., Maier, M.S. "Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata" . Mycological Progress 17, no. 4 (2018) : 479-487.
http://dx.doi.org/10.1007/s11557-017-1374-1
---------- MLA ----------
Fazio, A.T., Adler, M.T., Parnmen, S., Lücking, R., Maier, M.S. "Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata" . Mycological Progress, vol. 17, no. 4, 2018, pp. 479-487.
http://dx.doi.org/10.1007/s11557-017-1374-1
---------- VANCOUVER ----------
Fazio, A.T., Adler, M.T., Parnmen, S., Lücking, R., Maier, M.S. Production of the bioactive pigment elsinochrome A by a cultured mycobiont strain of the lichen Graphis elongata. Mycol. Prog. 2018;17(4):479-487.
http://dx.doi.org/10.1007/s11557-017-1374-1