Artículo

Bárcena, A.; Petroselli, G.; Velasquez, S.M.; Estévez, J.M.; Erra-Balsells, R.; Balatti, P.A.; Saparrat, M.C.N. "Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole" (2015) Mycological Progress. 14(9)
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Abstract:

Pseudocercospora griseola, an anamorph of Mycosphaerella, causes Angular Leaf Spot (ALS). The mycelia and conidia from P. griseola are coloured due to the synthesis of 1,8 dihydroxynaphthalene (DHN)-melanin. The aim of this work was to identify in P. griseola f. mesoamericana isolate T4, a highly pigmented fungus, intermediary compounds as a result of the inhibition of melanin synthesis by tricyclazole, and to analyze at the structural level the localization of these dark pigments. The main metabolites were analyzed using ultraviolet matrix-assisted laser desorption-ionization mass spectrometry (UV–MALDI MS). Tricyclazole affected P. griseola f. mesoamericana in several different ways. The most evident effect was the reduction of melanin synthesis, and therefore diffusible shunt products were found and identified. Flaviolin was the main intermediate metabolite found in cultures supplemented with tricyclazole. This inhibitor, which affected pigmentation and the cell wall structure of mycelium, revealed macroscopically by the reduction in growth, decreased the stratification and deposition of melanin in the hyphal wall. These results suggest a possible role of tricyclazole to control ALS. © 2015, German Mycological Society and Springer-Verlag Berlin Heidelberg.

Registro:

Documento: Artículo
Título:Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole
Autor:Bárcena, A.; Petroselli, G.; Velasquez, S.M.; Estévez, J.M.; Erra-Balsells, R.; Balatti, P.A.; Saparrat, M.C.N.
Filiación:Instituto de Fisiología Vegetal (INFIVE), Universidad Nacional de La Plata (UNLP)-CCT-La Plata-Consejo Nacional de Investigaciones Científicas y técnicas (CONICET), Diag. 113 y 61, CC 327, La Plata, 1900, Argentina
CIHIDECAR-CONICET, Departamento de Química Orgánica, Universidad de Buenos Aires, Pabellón II, 3 Ciudad Universitaria, Buenos Aires, 1428, Argentina
Fundación Instituto Leloir, Av. Patricias Argentinas 435, Buenos Aires, C1405BWE, Argentina
Cátedra de Microbiología Agrícola, Universidad Nacional de La Plata, 60 y 119, La Plata, 1900, Argentina
Cátedra de Fitopatología-CIDEFI, Universidad Nacional de La Plata, 60 y 119, CC31, La Plata, 1900, Argentina
Instituto de Botánica Spegazzini, Universidad Nacional de La Plata, 53 # 477, La Plata, 1900, Argentina
Palabras clave:FTIR; Melanin; Microscopy; Phaseolus vulgaris; Pseudocercospora griseola f. mesoamericana; Tricyclazole; UV-MALDI-TOF MS; Fungi; Mycosphaerella; Phaseolus vulgaris; Pseudocercospora
Año:2015
Volumen:14
Número:9
DOI: http://dx.doi.org/10.1007/s11557-015-1102-7
Título revista:Mycological Progress
Título revista abreviado:Mycol. Prog.
ISSN:1617416X
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1617416X_v14_n9_p_Barcena

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Citas:

---------- APA ----------
Bárcena, A., Petroselli, G., Velasquez, S.M., Estévez, J.M., Erra-Balsells, R., Balatti, P.A. & Saparrat, M.C.N. (2015) . Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole. Mycological Progress, 14(9).
http://dx.doi.org/10.1007/s11557-015-1102-7
---------- CHICAGO ----------
Bárcena, A., Petroselli, G., Velasquez, S.M., Estévez, J.M., Erra-Balsells, R., Balatti, P.A., et al. "Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole" . Mycological Progress 14, no. 9 (2015).
http://dx.doi.org/10.1007/s11557-015-1102-7
---------- MLA ----------
Bárcena, A., Petroselli, G., Velasquez, S.M., Estévez, J.M., Erra-Balsells, R., Balatti, P.A., et al. "Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole" . Mycological Progress, vol. 14, no. 9, 2015.
http://dx.doi.org/10.1007/s11557-015-1102-7
---------- VANCOUVER ----------
Bárcena, A., Petroselli, G., Velasquez, S.M., Estévez, J.M., Erra-Balsells, R., Balatti, P.A., et al. Response of the fungus Pseudocercospora griseola f. mesoamericana to Tricyclazole. Mycol. Prog. 2015;14(9).
http://dx.doi.org/10.1007/s11557-015-1102-7