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Abstract:

The "algarrobo" [Prosopis chilensis (Molina) Stuntz] is a tree species that represents an important natural resource in arid and semi-arid regions of Argentina. In this paper, we analysed and compared the variability of 46 RAPD (Random Amplified Polymorphic DNA) loci with previous estimates obtained from 12 isozyme markers in nine Argentinean populations of P. chilensis representative of the whole range of this species in Argentina. We evaluated the population structure and the existence of genetic variants associated with environmental variables. Expected heterozygosity (He) estimated from RAPD varied significantly among populations and regions. Hierarchical analysis of genetic variability (AMOVA) showed that most (88.1%) of the total diversity occurs within populations, the component among populations within regions (9.3%) was intermediate, while the between-region component was the lowest (2.6%). All three variance components were highly significant. The MDS plot from pair-wise ΦST matrix was consistent with the highly significant among-region differentiation indicated by the AMOVA. All 12 variable isozyme loci and 26 out of 46 RAPD loci showed significant or highly significant association with at least one geographic/climatic variable according to the stepwise multiple regression analysis. These results imply that the genetic differentiation among populations is better explained by environmental or biogeographical grounds than by geographical distances, suggesting gametic disequilibrium with loci responsible for the adaptation to particular environmental conditions. The information from RAPD markers would provide a relevant criterion to preserve genetic diversity in programmes for conservation and rationale use of this species. © 2010 Springer-Verlag.

Registro:

Documento: Artículo
Título:Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers
Autor:Ferreyra, L.I.; Vilardi, J.C.; Tosto, D.S.; Julio, N.B.; Saidman, B.O.
Filiación:Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Intendente Guiraldes 2160, C1428EGA, Buenos Aires, Argentina
Laboratorio de Genética de Poblaciones Aplicada, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Biotecnología, INTA-Castelar, Dr. N. Repetto y de los Reseros 1686 Hurlingham, Buenos Aires, Argentina
Cátedra de Genética, Facultad de Ciencias Exactas, Físicas, Universidad Nacional de Córdoba, Av. Vélez Sarsfield 299, 5000 Córdoba, Argentina
Laboratorio de Genética, Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Environment factors; Genetic structure; Genetic variability; Homology; Isozyme; Prosopis chilensis; RAPD; RAPD bands; angiosperm; DNA; environmental conditions; environmental factor; genetic differentiation; genetic variation; heterozygosity; homology; population structure; Argentina; Prosopis; Prosopis chilensis
Año:2010
Volumen:129
Número:6
Página de inicio:1011
Página de fin:1025
DOI: http://dx.doi.org/10.1007/s10342-010-0386-z
Título revista:European Journal of Forest Research
Título revista abreviado:Eur. J. For. Res.
ISSN:16124669
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_16124669_v129_n6_p1011_Ferreyra

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Citas:

---------- APA ----------
Ferreyra, L.I., Vilardi, J.C., Tosto, D.S., Julio, N.B. & Saidman, B.O. (2010) . Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers. European Journal of Forest Research, 129(6), 1011-1025.
http://dx.doi.org/10.1007/s10342-010-0386-z
---------- CHICAGO ----------
Ferreyra, L.I., Vilardi, J.C., Tosto, D.S., Julio, N.B., Saidman, B.O. "Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers" . European Journal of Forest Research 129, no. 6 (2010) : 1011-1025.
http://dx.doi.org/10.1007/s10342-010-0386-z
---------- MLA ----------
Ferreyra, L.I., Vilardi, J.C., Tosto, D.S., Julio, N.B., Saidman, B.O. "Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers" . European Journal of Forest Research, vol. 129, no. 6, 2010, pp. 1011-1025.
http://dx.doi.org/10.1007/s10342-010-0386-z
---------- VANCOUVER ----------
Ferreyra, L.I., Vilardi, J.C., Tosto, D.S., Julio, N.B., Saidman, B.O. Adaptive genetic diversity and population structure of the "algarrobo" [Prosopis chilensis (Molina) Stuntz] analysed by RAPD and isozyme markers. Eur. J. For. Res. 2010;129(6):1011-1025.
http://dx.doi.org/10.1007/s10342-010-0386-z